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Team:Harvard/allergy/parts
From 2010.igem.org
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<div id="abstract"> | <div id="abstract"> | ||
<h2>Allergy</h2> | <h2>Allergy</h2> | ||
| - | For more information on the allergens visit our "<a href="allergens"> | + | For more information on the allergens visit our "<a href="allergens">allergen targets</a>" page<br /><br /> |
<table cellspacing="0"> | <table cellspacing="0"> | ||
| - | <tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr> | + | <tr><td><b>Name</b></td><td><b>Biobrick Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr> |
| - | <tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td><a href="AmirnaGFP.txt"> | + | <tr><td>A1</td><td><center>-</center></td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td><a href="AmirnaGFP.txt"> amiRNA GFP</a></td></tr> |
| - | <tr><td>A2</td><td>amiRNA | + | <tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>chloramphenicol</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr> |
| - | <tr><td>A3</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td><a href="Bet1.txt"> ihpRNA Bet1</a></td></tr> | + | <tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td><a href="Bet1.txt"> ihpRNA Bet1</a></td></tr> |
| - | <tr><td>A4</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr> | + | <tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr> |
| - | <tr><td>A5</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr> | + | <tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr> |
</table> | </table> | ||
<br /><br /> | <br /><br /> | ||
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<tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer3</a></td></tr> | <tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer3</a></td></tr> | ||
<tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer4</a></td></tr> | <tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer4</a></td></tr> | ||
| - | <tr><td>AP5</td><td>1 of 4 primers used to create the amiRNA | + | <tr><td>AP5</td><td>1 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 1</a></td></tr> |
| - | <tr><td>AP6</td><td>2 of 4 primers used to create the amiRNA | + | <tr><td>AP6</td><td>2 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 2</a></td></tr> |
| - | <tr><td>AP7</td><td>3 of 4 primers used to create the amiRNA | + | <tr><td>AP7</td><td>3 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 3</a></td></tr> |
| - | <tr><td>AP8</td><td>4 of 4 primers used to create the amiRNA | + | <tr><td>AP8</td><td>4 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 4</a></td></tr> |
<tr><td>AP9</td><td>Forward primer for Bet v1 Sense</td><td><a href="Bet1.txt"> Bet1 Sense Forward</a></td></tr> | <tr><td>AP9</td><td>Forward primer for Bet v1 Sense</td><td><a href="Bet1.txt"> Bet1 Sense Forward</a></td></tr> | ||
<tr><td>AP10</td><td>Reverse primer for Bet v1 Sense </td><td><a href="Bet1.txt"> Bet1 Sense Reverse</a></td></tr> | <tr><td>AP10</td><td>Reverse primer for Bet v1 Sense </td><td><a href="Bet1.txt"> Bet1 Sense Reverse</a></td></tr> | ||
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<tr><td>AP19</td><td>Forward primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Forward</a></td></tr> | <tr><td>AP19</td><td>Forward primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Forward</a></td></tr> | ||
<tr><td>AP20</td><td>Reverse primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Reverse</a></td></tr> | <tr><td>AP20</td><td>Reverse primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Reverse</a></td></tr> | ||
| - | <tr><td>AP21</td><td>Forward Primer for PDK intron</td><td></td></tr> | + | <tr><td>AP21</td><td>Forward Primer for PDK intron</td><td><a href="Introns"> PDK forward</a></td></tr> |
| - | <tr><td>AP22</td><td>Reverse Primer for PDK intron</td><td></td></tr> | + | <tr><td>AP22</td><td>Reverse Primer for PDK intron</td><td><a href="Introns"> PDK reverse</a></td></tr> |
| - | <tr><td>AP23</td><td>Forward Primer to make amiRNA Biobrick compatible</td><td></td></tr> | + | <tr><td>AP23</td><td>Forward Primer to make amiRNA Biobrick compatible</td><td><a href="amiRNA"> Biobrick forward</a></td></tr> |
| - | <tr><td>AP24</td><td>Reverse Primer to make amiRNA Biobrick compatible</td><td></td></tr> | + | <tr><td>AP24</td><td>Reverse Primer to make amiRNA Biobrick compatible</td><td><a href="amiRNA"> Biobrick reverse</a></td></tr> |
| - | <tr><td>AP25</td><td>1 of 4 primers used to create the amiRNA | + | <tr><td>AP25</td><td>1 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 1</a></td></tr> |
| - | <tr><td>AP26</td><td>2 of 4 primers used to create the amiRNA | + | <tr><td>AP26</td><td>2 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 2</a></td></tr> |
| - | <tr><td>AP27</td><td>3 of 4 primers used to create the amiRNA | + | <tr><td>AP27</td><td>3 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 3</a></td></tr> |
| - | <tr><td>AP28</td><td>4 of 4 primers used to create the amiRNA | + | <tr><td>AP28</td><td>4 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 4</a></td></tr> |
Latest revision as of 00:04, 27 October 2010
parts and primers
Allergy
For more information on the allergens visit our "allergen targets" page| Name | Biobrick Name | Original Name | Description | Resistance | Sequence |
| A1 | amiRNA GFP | GFP knockdown construct | chloramphenicol | amiRNA GFP | |
| A2 | BBa_K382017 | amiRNA LTP | LTP knockdown construct | chloramphenicol | amiRNA LTP |
| A3 | BBa_K382014 | ihpRNA Bet | Intron hairpin Bet Knockdown construct | chloramphenicol | ihpRNA Bet1 |
| A4 | BBa_K382015 | ihpRNA LTP | Intron hairpin LTP Knockdown construct | chloramphenicol | ihpRNA LTP |
| A5 | BBa_K382019 | ihpRNA Ger | Intron hairpin Ger Knockdown construct | chloramphenicol | ihpRNA GER |
Primers
| Name | Description | Sequence |
| AP1 | 1 of 4 primers used to create the amiRNA GFP construct | GFP primer1 |
| AP2 | 2 of 4 primers used to create the amiRNA GFP construct | GFP primer2 |
| AP3 | 3 of 4 primers used to create the amiRNA GFP construct | GFP primer3 |
| AP4 | 4 of 4 primers used to create the amiRNA GFP construct | GFP primer4 |
| AP5 | 1 of 4 primers used to create the amiRNA LTP construct | Bet1 Primer 1 |
| AP6 | 2 of 4 primers used to create the amiRNA LTP construct | Bet1 Primer 2 |
| AP7 | 3 of 4 primers used to create the amiRNA LTP construct | Bet1 Primer 3 |
| AP8 | 4 of 4 primers used to create the amiRNA LTP construct | Bet1 Primer 4 |
| AP9 | Forward primer for Bet v1 Sense | Bet1 Sense Forward |
| AP10 | Reverse primer for Bet v1 Sense | Bet1 Sense Reverse |
| AP11 | Forward primer for Bet v1 Antisense | Bet1 Antisense Forward |
| AP12 | Reverse primer for Bet v1 Antisense | Bet1 Antisense Reverse |
| AP13 | Forward primer for LTP Sense | LTP Sense Forward |
| AP14 | Reverse primer for LTP Sense | LTP Sense Reverse |
| AP15 | Forward primer for LTP Antisense | LTP Antisense Forward |
| AP16 | Reverse primer for LTP Antisense | LTP Antisense Reverse |
| AP17 | Forward primer for Ger Sense | Ger Sense Forward |
| AP18 | Reverse primer for Ger Sense | Ger Sense Reverse |
| AP19 | Forward primer for Ger Antisense | Ger Antisense Forward |
| AP20 | Reverse primer for Ger Antisense | Ger Antisense Reverse |
| AP21 | Forward Primer for PDK intron | PDK forward |
| AP22 | Reverse Primer for PDK intron | PDK reverse |
| AP23 | Forward Primer to make amiRNA Biobrick compatible | Biobrick forward |
| AP24 | Reverse Primer to make amiRNA Biobrick compatible | Biobrick reverse |
| AP25 | 1 of 4 primers used to create the amiRNA Bet construct | LTP primer 1 |
| AP26 | 2 of 4 primers used to create the amiRNA Bet construct | LTP primer 2 |
| AP27 | 3 of 4 primers used to create the amiRNA Bet construct | LTP primer 3 |
| AP28 | 4 of 4 primers used to create the amiRNA Bet construct | LTP primer 4 |
