Team:Tokyo Metropolitan/Notebook/Fiber/2010/10/06

From 2010.igem.org

(Difference between revisions)
(→Experiment:Measure DNA density)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Tokyo_Metropolitan/Header}}
+
{{:Team:Tokyo_Metropolitan/Notebook/Fiber}}
-
 
+
__NOTOC__
==2010/10/06 Wednesday (Naoto)==
==2010/10/06 Wednesday (Naoto)==
===Experiment:Electrophoresis of insert check PCR===
===Experiment:Electrophoresis of insert check PCR===
-
'''member'''
+
:'''member'''
-
naoto
+
:naoto
-
'''material'''
+
:'''material'''
-
*insert check PCR production(PCR at 10/05)  
+
:*insert check PCR production(PCR at 10/05)  
-
If you want to know other materials,see protocol4
+
:If you want to know other materials,see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
-
'''procedure'''
+
:'''procedure'''
-
see protocol4
+
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol4:Agarose_gel_electrophoresis protocol4]
-
'''result'''
+
:'''result'''
-
All of bands seemed to be vector self ligation
+
:All of bands seemed to be vector self ligation
===Experiment:PCR of  T7promoter~bcsABCD(''A.xylinum''),T7promoter~bcsEFG(''E.coli''),RBS~T7polymerase===
===Experiment:PCR of  T7promoter~bcsABCD(''A.xylinum''),T7promoter~bcsEFG(''E.coli''),RBS~T7polymerase===
-
'''member'''
+
:'''member'''
-
naoto
+
:naoto
-
'''material'''
+
:'''material'''
-
*A colony of ''A.xylinum''
+
:*A colony of ''A.xylinum''
-
*bcsEFG(''E.coli'')
+
:*bcsEFG(''E.coli'')
-
*T7 polymerase(BBa_K145001)
+
:*T7 polymerase(BBa_K145001)
-
If you want to know other materials,see protocol3
+
:If you want to know other materials,see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
-
'''procedure'''
+
:'''procedure'''
-
see protocol3
+
:see [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Protocol3:PCR protocol3]
===Experiment:Measure DNA density===
===Experiment:Measure DNA density===
-
'''member'''
+
:'''member'''
 +
 
 +
:naoto
 +
 
 +
:'''material'''
 +
 
 +
:*absorption spectrometer
 +
:*bcsA,B,C,D(''A.xylinum'')
 +
:*bcsA,B,C,EFG(''E.coli'')
 +
:*pSB1C3
 +
:*TE buffer
 +
 
 +
:'''procedure'''
-
naoto
+
:*mix 2µl DNA solution with 2μl TE buffer
 +
:*apply DNA solution and TE buffer mixture to absorption spectrometer
 +
:*measure DNA density
-
'''material'''
 
-
*absorption spectrometer
 
-
*bcsA,B,C,D(''A.xylinum'')
 
-
*bcsA,B,C,EFG(''E.coli'')
 
-
*pSB1C3
 
-
*TE buffer
 
-
'''procedure'''
 
-
*mix 2µl DNA solution with 2μl TE buffer
+
<br />
-
*apply DNA solution and TE buffer mixture to absorption spectrometer
+
-
*measure DNA density
+

Latest revision as of 18:13, 27 October 2010


E.coli Fiber Project Notebook

August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

2010/10/06 Wednesday (Naoto)

Experiment:Electrophoresis of insert check PCR

member
naoto
material
  • insert check PCR production(PCR at 10/05)
If you want to know other materials,see protocol4
procedure
see protocol4
result
All of bands seemed to be vector self ligation

Experiment:PCR of T7promoter~bcsABCD(A.xylinum),T7promoter~bcsEFG(E.coli),RBS~T7polymerase

member
naoto
material
  • A colony of A.xylinum
  • bcsEFG(E.coli)
  • T7 polymerase(BBa_K145001)
If you want to know other materials,see protocol3
procedure
see protocol3

Experiment:Measure DNA density

member
naoto
material
  • absorption spectrometer
  • bcsA,B,C,D(A.xylinum)
  • bcsA,B,C,EFG(E.coli)
  • pSB1C3
  • TE buffer
procedure
  • mix 2µl DNA solution with 2μl TE buffer
  • apply DNA solution and TE buffer mixture to absorption spectrometer
  • measure DNA density




Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/10/06"