Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/10

From 2010.igem.org

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===2010/10/10===
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{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
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=2010/10/10=
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===1:Electrophoresis===
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<Member><br />
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nito
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<Sample><br />
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PCR products (10/10)
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 +
<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
<Result><br />
<Result><br />
Line 7: Line 18:
<Result><br />
<Result><br />
[[Image:Matsuura_2010-10-10_21hr_24min.JPG]]
[[Image:Matsuura_2010-10-10_21hr_24min.JPG]]
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[[Image:]]
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[[Image:]][[Image:]]
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<Result><br />
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[[Image:]]
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[[Image:Matsuura_2010-10-10_18hr_40min.JPG]]
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<Result><br />
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[[Image: Image:Matsuura 2010-10-10 21hr 24min.JPG]]
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===2:PCR===
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<Member><br />
 +
nito
 +
 
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<Sample>(temperature in annealing:℃)<br />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] (60)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] (60)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] (60)
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] (60)
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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===3：DNA Digestion===
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＜Member＞<br />
 +
nito
 +
 
 +
＜Sample, Materials＞<br />
 +
・PCR productions
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
 +
 
 +
 
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＜Protocol＞<br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
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===4：DNA Ligation ===
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＜Member＞<br />
 +
nito
 +
 
 +
＜Sample＞<br />
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] 
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
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(after digestion)
 +
 
 +
＜Protocol＞<br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
 +
 
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===5：DNA extraction===
 +
＜Member＞<br />
 +
Mariko,mio,nito
 +
 
 +
＜Sample＞<br />
 +
PCR products
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
 +
 
 +
＜Protocol＞<br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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 +
===6:Plasmid extraction from E-coli===
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<Member><br />
 +
nito
 +
 
 +
<Sample><br />
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 12]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 13]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 15]
 +
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 16]
 +
 
 +
<Protocol><br />
 +
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]

Latest revision as of 02:58, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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October 2010
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Contents

2010/10/10

1:Electrophoresis

<Member>
nito

<Sample>
PCR products (10/10)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-10 11hr 40min.JPG

<Result>
Matsuura 2010-10-10 21hr 24min.JPG

<Result>
Matsuura 2010-10-10 18hr 40min.JPG

<Result>
File:Image:Matsuura 2010-10-10 21hr 24min.JPG


2:PCR

<Member>
nito

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 1

3：DNA Digestion

＜Member＞
nito

＜Sample, Materials＞
・PCR productions


＜Protocol＞
See Prptpcpl 9

4：DNA Ligation

＜Member＞
nito

＜Sample＞

(after digestion)

＜Protocol＞
See Protocol 10

5：DNA extraction

＜Member＞
Mariko,mio,nito

＜Sample＞
PCR products

＜Protocol＞
See Protocol 4

6:Plasmid extraction from E-coli

<Member>
nito

<Sample>

<Protocol>
See Protocol 6

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/10/10"