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BIOTEC Dresden/Notepad/14 September 2010
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Charansam21 (Talk | contribs) (New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> '''Parts assembly''' ...) |
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No growth of colonies from the plating done on 13.09.2010. Ligation did not work. This might have been due to the inefficiency of the XbaI restriction enzyme in DH5 alpha cells (competent cells used during transformation). | No growth of colonies from the plating done on 13.09.2010. Ligation did not work. This might have been due to the inefficiency of the XbaI restriction enzyme in DH5 alpha cells (competent cells used during transformation). | ||
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'''Fusion Protein''' | '''Fusion Protein''' | ||
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A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion. | A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion. | ||
| - | + | '''AHL Sensor''' | |
| + | <p>Transformation of the 17+51 ligated part was repeated</p> | ||
| + | <p>Colonies were picked from yesterday's transformation for colony PCR</p> | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 16:41, 27 October 2010
Parts assembly
No growth of colonies from the plating done on 13.09.2010. Ligation did not work. This might have been due to the inefficiency of the XbaI restriction enzyme in DH5 alpha cells (competent cells used during transformation).
Fusion Protein
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.
AHL Sensor
Transformation of the 17+51 ligated part was repeated
Colonies were picked from yesterday's transformation for colony PCR
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