Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/02
From 2010.igem.org
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=2010/10/02= | =2010/10/02= | ||
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<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
Transformation 9/28 | Transformation 9/28 | ||
- | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
- | *7-8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
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===2:DNA Ligation === | ===2:DNA Ligation === | ||
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<Sample><br /> | <Sample><br /> | ||
- | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
- | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
(after digestion) | (after digestion) | ||
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
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===3:Electrophoresis=== | ===3:Electrophoresis=== | ||
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<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
- | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
- | *7-8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8] |
<Protocol><br /> | <Protocol><br /> |
Latest revision as of 02:55, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/10/02
1:PCR
<Member>
mio,mizuki
<Sample>(temperature in annealing:℃)
Transformation 9/28
<Protocol>
See Protocol 2
2:DNA Ligation
<Member>
mizuki
<Sample>
(after digestion)
<Protocol>
See Protocol 10
3:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products
<Protocol>
See Protocol 8