Team:Tokyo Metropolitan/Notebook/Fiber/2010/09/21

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__NOTOC__
==2010/9/21 Tuesday (watachin)==
==2010/9/21 Tuesday (watachin)==
===Experiment:Miniprep (extraction of plasmid kit)===
===Experiment:Miniprep (extraction of plasmid kit)===
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'''Member'''<br />
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:'''Member'''<br />
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easily and NEX
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:easily and NEX
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+
-
'''Materials and Procedure'''
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Miniprep_.28extraction_of_plasmid_kit.29">the protocol(Miniprep)</a></html>
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:'''Materials and Procedure'''
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Miniprep_.28extraction_of_plasmid_kit.29">protocol10</a></html>
===Experiment:PCR===
===Experiment:PCR===
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'''Member'''<br />
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:'''Member'''<br />
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watachin
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:watachin
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'''Materials and Procedure'''
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:'''Materials and Procedure'''
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+
-
PCR of pSB1C3
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-
 
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">the protocol(Direct PCR)</a></html>
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 +
:PCR of pSB1C3
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#.E3.80.80Direct_PCR">protocol3</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
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'''Member'''<br />
+
:'''Member'''<br />
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easily
+
:easily
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'''Materials and Procedure'''
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:'''Materials and Procedure'''
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
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'''Result'''
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:'''Result'''
[[Image:2010-09-21-01.jpg]]
[[Image:2010-09-21-01.jpg]]
[[Image:2010-09-21-02.jpg]]
[[Image:2010-09-21-02.jpg]]
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===Experiment:DNA purification from agarose gel with QIAGEN===
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===Experiment:DNA purification from agarose gel===
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'''Member'''<br />
+
:'''Member'''<br />
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watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
 
+
-
 
+
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
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 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
===Experiment:Digestion===
===Experiment:Digestion===
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'''Member'''<br />
+
:'''Member'''<br />
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NEX and watachin
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:NEX and watachin
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'''Materials and Procedure'''
+
:'''Materials and Procedure'''
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">the protocol(Digestion)</a></html>
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===Experiment:Digestion===
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'''Member'''<br />
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-
NEX and watachin
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-
 
+
-
'''Materials and Procedure'''
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-
 
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">the protocol(Digestion)</a></html>
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 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Digestion">protocol7</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
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'''Member'''<br />
+
:'''Member'''<br />
-
NEX and watachin
+
:NEX and watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol5</a></html>
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
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:'''Result'''
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+
-
 
+
-
'''Result'''
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[[Image:2010-09-21-03.jpg]]
[[Image:2010-09-21-03.jpg]]
 +
===Experiment:DNA Extraction from agarose gel===
 +
:'''Member'''<br />
 +
:NEX and watachin
-
===Experiment:DNA purification from agarose gel with QIAGEN===
+
:'''Materials and Procedure'''
-
'''Member'''<br />
+
-
NEX and watachin
+
-
 
+
-
'''Materials and Procedure'''
+
-
 
+
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
===Experiment:Electrophoresis===
===Experiment:Electrophoresis===
-
'''Member'''<br />
+
:'''Member'''<br />
-
watachin
+
:watachin
-
'''Materials and Procedure'''
+
:'''Materials and Procedure'''
 +
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">protocol4</a></html>
-
follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Electrophoresis">the protocol(Electrophoresis)</a></html>
+
:'''Result'''
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+
-
 
+
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'''Result'''
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[[Image:2010-09-21-04.jpg]]
[[Image:2010-09-21-04.jpg]]
[[Image:2010-09-21-05.jpg]]
[[Image:2010-09-21-05.jpg]]
 +
===Experiment:DNA Extraction from agarose gel===
 +
:'''Member'''<br />
 +
:watachin
-
===Experiment:DNA purification from agarose gel with QIAGEN===
+
:'''Materials and Procedure'''
-
'''Member'''<br />
+
-
watachin
+
-
'''Materials and Procedure'''
+
:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">protocol5</a></html>
-
 
+
-
 
+
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#DNA_purification_from_agarose_gel_with_QIAGEN">the protocol(DNA purification from agarose gel with QIAGEN)</a></html>
+
===Experiment:Transformation===
===Experiment:Transformation===
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'''Member'''<br />
+
:'''Member'''<br />
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watachin
+
:watachin
-
 
+
-
'''Materials and Procedure'''
+
 +
:'''Materials and Procedure'''
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follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">the protocol(Transformation)</a></html>
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:follow <html><a href="https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Fiber/Protocol#Transformation">protocol9</a></html>

Latest revision as of 14:02, 27 October 2010


E.coli Fiber Project Notebook

August 2010
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September 2010
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October 2010
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2010/9/21 Tuesday (watachin)

Experiment:Miniprep (extraction of plasmid kit)

Member
easily and NEX
Materials and Procedure
follow protocol10

Experiment:PCR

Member
watachin
Materials and Procedure
PCR of pSB1C3
follow protocol3

Experiment:Electrophoresis

Member
easily
Materials and Procedure
follow protocol4
Result

2010-09-21-01.jpg 2010-09-21-02.jpg

Experiment:DNA purification from agarose gel

Member
watachin
Materials and Procedure
follow protocol5

Experiment:Digestion

Member
NEX and watachin
Materials and Procedure
follow protocol7

Experiment:Electrophoresis

Member
NEX and watachin
Materials and Procedure
follow protocol5
Result

2010-09-21-03.jpg

Experiment:DNA Extraction from agarose gel

Member
NEX and watachin
Materials and Procedure
follow protocol5

Experiment:Electrophoresis

Member
watachin
Materials and Procedure
follow protocol4
Result

2010-09-21-04.jpg 2010-09-21-05.jpg

Experiment:DNA Extraction from agarose gel

Member
watachin
Materials and Procedure
follow protocol5

Experiment:Transformation

Member
watachin
Materials and Procedure
follow protocol9
Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Fiber/2010/09/21"