Team:Michigan/Alex's Notebook
From 2010.igem.org
(Difference between revisions)
m (→10/3/10) |
|||
(4 intermediate revisions not shown) | |||
Line 621: | Line 621: | ||
--[[User:Infekt|Infekt]] 00:57, 1 October 2010 (UTC) | --[[User:Infekt|Infekt]] 00:57, 1 October 2010 (UTC) | ||
+ | |||
+ | == 10/1/10 == | ||
+ | ''Alex'' | ||
+ | |||
+ | Moved broth cultures 37°C to 4°C -- 1:30pm (19-hr culture) | ||
+ | Prepared biofilm assay plate for all pH9 tests - following [[Media:Static+biofilm+quantification.pdf|Alex's biofilm assay protocol]] | ||
+ | *plate template: | ||
+ | [[image:9-30 plate template.jpg]] | ||
+ | **100 μL/well: 99 μL medium + 1 μL culture | ||
+ | *stored plate in Lin Lab 4°C -- 2:20pm | ||
+ | ---- | ||
+ | Saved data for curves from ph7 plate | ||
+ | Read pH7 plate ODs | ||
+ | Performed biofilm assay on ph7 plate | ||
+ | Started 24-hr kinetic reading for pH9 plate -- ~midnight | ||
+ | |||
+ | Discarded pH7 plate | ||
+ | |||
+ | Processed pH7 plate data | ||
+ | *uploaded results [[Media:Ph7_biofilm_assay_results.xls|here]] | ||
+ | |||
+ | --[[User:Infekt|Infekt]] 06:55, 2 October 2010 (UTC) | ||
+ | |||
+ | Uploaded pH7 kinetic data [[Media:Data_10-01-10-pH7_kinetic.txt|here]] | ||
+ | |||
+ | --[[User:Infekt|Infekt]] 04:06, 4 October 2010 (UTC) | ||
+ | |||
+ | == 10/3/10 == | ||
+ | ''Alex'' | ||
+ | |||
+ | Ran CV biofilm assay on pH9 plate, following [[Media:Static+biofilm+quantification.pdf|my protocol]] | ||
+ | *discarded plate | ||
+ | *uploaded [[Media:Ph9_biofilm_assay_results.xls|results]] | ||
+ | *uploaded [[Media:Data_10-01-10-pH9_kinetic.txt|pH9 kinetic raw data]] | ||
+ | |||
+ | --[[User:Infekt|Infekt]] 04:35, 4 October 2010 (UTC) |
Latest revision as of 04:36, 4 October 2010