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Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/27
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(Difference between revisions)
(New page: ===1:PCR=== <Member><br /> Hitomi <Sample>(temperature in annealing)<br /> *7 (66.5) *8 (66.5) *9 (61) *10(66.5) <Protocol><br /> See [https://2010.igem.org/Team:Tokyo_Metropolitan/Projec...) |
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| + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} | ||
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| + | =2010/09/27= | ||
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===1:PCR=== | ===1:PCR=== | ||
<Member><br /> | <Member><br /> | ||
| Line 4: | Line 8: | ||
<Sample>(temperature in annealing)<br /> | <Sample>(temperature in annealing)<br /> | ||
| - | *7 (66.5) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] (66.5) |
| - | *8 (66.5) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] (66.5) |
| - | *9 (61) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] (61) |
| - | *10(66.5) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10](66.5) |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| - | |||
===2:Electrophoresis=== | ===2:Electrophoresis=== | ||
| Line 19: | Line 22: | ||
<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
Ligation products | Ligation products | ||
| - | *7-8-10(9/26) | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10](9/26) |
<Protocol><br /> | <Protocol><br /> | ||
| Line 28: | Line 31: | ||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-09-27_21hr_03min.JPG]] |
| - | + | ||
===3:PCR=== | ===3:PCR=== | ||
| Line 36: | Line 38: | ||
<Sample>(temperature in annealing)<br /> | <Sample>(temperature in annealing)<br /> | ||
| - | *8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| - | |||
===4：DNA Digestion=== | ===4：DNA Digestion=== | ||
| Line 47: | Line 48: | ||
＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
| - | *7-8-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
*vector | *vector | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
Latest revision as of 02:54, 25 October 2010
E.coli Pattern Formation Project Notebook
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Contents |
2010/09/27
1:PCR
<Member>
Hitomi
<Sample>(temperature in annealing)
<Protocol>
See Protocol 1
2:Electrophoresis
<Member>
mizuki
<Sample>
PCR products
Ligation products
- 7-8-10(9/26)
<Protocol>
See Protocol 8
<Result>
3:PCR
<Member>
mizuki
<Sample>(temperature in annealing)
<Protocol>
See Protocol 1
4：DNA Digestion
＜Member＞
mio
＜Sample, Materials＞
＜Protocol＞
See Prptpcpl 9
