Team:UC Davis/notebook/week4.html

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<li>For the light sensor, the RBS was extracted successfully from the gel; but promoters did not show
<li>For the light sensor, the RBS was extracted successfully from the gel; but promoters did not show
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A new workplan for the light sensor because extracting promoters of 35bp & 130bp will not be a good idea, thus, we present to you: Light Sensor Take Two </li><br />
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A new workplan for the light sensor because extracting promoters of 35bp & 130bp will not be a good idea, thus, we present to you: <a href="https://2010.igem.org/Team:UC_Davis/notebook/lightsensor2.html" class="help">Light Sensor Take Two </a></li><br />
<li>For the ON switch: (picture will be put up soon)</li>
<li>For the ON switch: (picture will be put up soon)</li>

Latest revision as of 04:46, 27 September 2010

Week 4: 7/4/10 through 7/10/10

  • Many workplans were revised
  • For the light sensor, the RBS was extracted successfully from the gel; but promoters did not show A new workplan for the light sensor because extracting promoters of 35bp & 130bp will not be a good idea, thus, we present to you: Light Sensor Take Two

  • For the ON switch: (picture will be put up soon)
  • |I12006(SP)-[B0034(SP)-C0051(XP)](XP)|(EX)-|[B0034(SP)-E1010(XP)](SP)-F1610(XP)|(EX)

  • For the initiator test construct, RBS-RFP will now be embedded in between the actual construct. To illustrate:

  • Actual construct: R0082-RBS-C0051-B0015
  • Test construct: R0082-RBS-RFP-RBS-C0051-B0015

  • Many, if not all ligations were unsuccessful: parts were not cutting successfully since bands in the gel were not showing up in the right places.

  • Week 4
We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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