Team:Aberdeen Scotland

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<h1>Project Abstract</h1>
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<h1>Why ayeSwitch?</h1>
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Our team aimed to engineer a genetic toggle switch that allows yeast to express either green or cyan fluorescent protein (GFP & CFP) mutually exclusively. This is regulated at the translational level which provides a more efficient and quicker response than previous switches regulated at the transcriptional level, bypassing mRNA synthesis and export. This is a novel mechanism previously untried. Our biology team successfully tested the RNA-binding protein/fluorescent protein expression in yeast, at the single cell and population levels; provided the modelling team parameters obtained from experimentation and research; and sequenced BioBricks fused to GFP and CFP in yeast expression cassettes. Using this data our modelling team successfully created a deterministic and a stochastic model of how we expected the toggle switch to behave and found that the maximum success rate for our original set of parameters was 0.96%, but through theoretical modifications an optimal parameter set predicted a maximum success rate of 51.27%. Unfortunately time constraints prevented implementation of these adjustments to the system, a procedure we would recommend for further research.
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Over the course of the summer, the University of Aberdeen iGEM team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters.  The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential.  These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.
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<a href="https://2010.igem.org/Team:Aberdeen_Scotland/Project_Overview">Your ayeSwitch experience begins here&nbsp;<img src="https://static.igem.org/mediawiki/2010/3/36/Right_arrow.png"></a>
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<h1>Our Sponsors:</h1>
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Aberdeen iGEM 2010 gratefully acknowledges the financial support of the following organisations:
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Latest revision as of 19:31, 27 October 2010

University of Aberdeen - ayeSwitch - iGEM 2010

Why ayeSwitch?

Over the course of the summer, the University of Aberdeen iGEM team engineered a novel genetic toggle switch in yeast which is regulated at the translational level and allows mutually exclusive expression of either green or cyan fluorescent protein. Using cell cytometry (FACS) and fluorimetry, we successfully demonstrated gene expression and translational regulation of a fusion of mRNA binding proteins and fluorescent proteins. Deterministic and stochastic models including experimental results and published parameter values predicted that the probability of successful bistability for the switch is 0.96%, but that this can theoretically be improved to a maximum of 51.27% by limiting the variation range of the most sensitive parameters. The models also predicted that to generate switch-like behaviour, co-operative binding of the mRNA binding protein to its mRNA stem loop was essential. These results suggest that a translationally regulated genetic toggle switch is a viable and novel engineering concept applicable to medicinal, environmental and technological problems.




Your ayeSwitch experience begins here 

Our Sponsors:

Aberdeen iGEM 2010 gratefully acknowledges the financial support of the following organisations:





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