Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test2settembre

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Latest revision as of 14:10, 27 October 2010

SEPTEMBER, 2ND

PLATE

EXPERIMENT DESCRIPTION

Motivation

This experiment was performed in M9 to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. (see Protocol) of the promoters from Anderson's collection in high copy and low copy number plasmids.

Methods

Accordign to Protocol 2 the experiment was performed in M9 on the following parts:

<partinfo>BBa_J23100</partinfo>
<partinfo>BBa_J23101</partinfo>
<partinfo>BBa_J23105</partinfo>
<partinfo>BBa_J23106</partinfo>
<partinfo>BBa_J23110</partinfo>
<partinfo>BBa_J23114</partinfo>
<partinfo>BBa_J23116</partinfo>
<partinfo>BBa_J23118</partinfo>
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5)
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5)
<partinfo>BBa_B0031</partinfo> (negative control)

RESULTS

In high copy number plasmids it is possible to identify 3 groups: strong promoters (<partinfo>Ba_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters (in order <partinfo>BBa_J23110</partinfo> and <partinfo>BBa_J23118</partinfo>) and a group of weak promoters.

Promoter	R.P.U. (average)	std
<partinfo>BBa_J23100</partinfo>	0.946	0.182
<partinfo>BBa_J23101</partinfo>	1	-
<partinfo>BBa_J23105</partinfo>	0.196	0.038
<partinfo>BBa_J23106</partinfo>	0.326	0.015
<partinfo>BBa_J23110</partinfo>	0.678	0.096
<partinfo>BBa_J23114</partinfo>	0.091	0.022
<partinfo>BBa_J23116</partinfo>	0.101	0.004
<partinfo>BBa_J23118</partinfo>	0.655	0.035

In low copy number plasmids there are the same 3 groups but <partinfo>BBa_J23118</partinfo> resulted stronger than <partinfo>BBa_J23110</partinfo>.

Promoter	R.P.U. (average)	std
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo>	0.838	0.101
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo>	1	-
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo>	0.149	0.045
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo>	0.286	0.033
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo>	0.466	0.101
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo>	0.053	0.048
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo>	0.110	0.007
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo>	0.714	0.154

Tecan Test

@@ Line 11: / Line 11: @@
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-<html><p align="center"><font size="5"><b>17° TEST</b></font><br><br><font size="4">[SEPTEMBER, 2ND]</font></p></html><hr><br>
+<html><p align="center"><font size="5"><b>SEPTEMBER, 2ND</b></font></p></html><hr><br>
 ==PLATE==
 <br>
-<!--[[Image:UNIPV_Pavia_piastra27agosto.png| 620px | center ]]-->
+[[Image:UNIPV_Pavia_piastra2settembre.png| 620px | center ]]
 <br>
 ==EXPERIMENT DESCRIPTION==
-==RESULTS (?)==
+===Motivation===
+This experiment was performed in M9 to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. ([[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for RPU evaluation|see Protocol]]) of the promoters from Anderson's collection in high copy and low copy number plasmids.
+===Methods===
+Accordign to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for constitutive promoters (R.P.U. evaluation) - Protocol #2|Protocol 2]] the experiment was performed in M9 on the following parts:
+* <partinfo>BBa_J23100</partinfo>
+* <partinfo>BBa_J23101</partinfo>
+* <partinfo>BBa_J23105</partinfo>
+* <partinfo>BBa_J23106</partinfo>
+* <partinfo>BBa_J23110</partinfo>
+* <partinfo>BBa_J23114</partinfo>
+* <partinfo>BBa_J23116</partinfo>
+* <partinfo>BBa_J23118</partinfo>
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5)
+* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5)
+* <partinfo>BBa_B0031</partinfo> (negative control)
+==RESULTS==
+[[Image:UNIPV_Pavia_HCpromotersM9.png| 620px | center ]]
+In high copy number plasmids it is possible to identify 3 groups: strong promoters (<partinfo>Ba_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters (in order <partinfo>BBa_J23110</partinfo> and <partinfo>BBa_J23118</partinfo>) and a group of weak promoters.
+{| border='1' align='center'
+|| Promoter || R.P.U. (average) || std
+|-
+|| <partinfo>BBa_J23100</partinfo> || 0.946 || 0.182
+|-
+|| <partinfo>BBa_J23101</partinfo> || 1 || -
+|-
+|| <partinfo>BBa_J23105</partinfo> || 0.196 || 0.038
+|-
+|| <partinfo>BBa_J23106</partinfo> || 0.326 || 0.015
+|-
+|| <partinfo>BBa_J23110</partinfo> || 0.678 || 0.096
+|-
+|| <partinfo>BBa_J23114</partinfo> || 0.091 || 0.022
+|-
+|| <partinfo>BBa_J23116</partinfo> || 0.101 || 0.004
+|-
+|| <partinfo>BBa_J23118</partinfo> || 0.655 || 0.035
+|}
+[[Image:UNIPV_Pavia_LCpromotersM9.png| 620px | center ]]
+In low copy number plasmids there are the same 3 groups but <partinfo>BBa_J23118</partinfo> resulted stronger than <partinfo>BBa_J23110</partinfo>.
+{| border='1' align='center'
+|| Promoter || R.P.U. (average) || std
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> || 0.838 || 0.101
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> || 1 || -
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> || 0.149 || 0.045
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> || 0.286 || 0.033
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> || 0.466 || 0.101
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> || 0.053 || 0.048
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> || 0.110 || 0.007
+|-
+|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> || 0.714 || 0.154
+|}
 <!-- table previous next test -->
@@ Line 24: / Line 98: @@
 <tr>
 <td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1settembre|Previous Test]]</td>
-<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test14settembre| Next Test]]</td>
+<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test8settembre| Next Test]]</td>
 </tr>
 </table>
@@ Line 34: / Line 108: @@
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-<table border="0" width="100%" height="100%">
+{{UNIPV-Pavia/Menu_measurments}}
-<tr align="right"><td height="15">
-[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
-</td></tr>
-<tr align="right"><td height="15">
-[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
-</td></tr>
-</table>
 </td>
 </table>