Team:UC Davis/notebook/week0705.html

From 2010.igem.org

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<a name="onswitch"><h1>ON switch progress</h1></a>
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<a name="onswitch"><h1>ON switch</h1></a>
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<ul>
<li>The following parts were digested: E1010, I13453, J23119, C0051, I12006 with EcoRI & SpeI </li>
<li>The following parts were digested: E1010, I13453, J23119, C0051, I12006 with EcoRI & SpeI </li>
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<li><a href="#week070510" class="help">Notebook entries: week of 7/05/10</a></li>
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<li><a href="#lightsensor" class="help">Light Sensor</a></li>
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<li><a href="#initiator" class="help">Initiator</a></li>
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<li><a href="#onswitch" class="help">On Switch</a></li>
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Latest revision as of 22:42, 16 September 2010

Week of 7/05/10

Light Sensor

Initiator
  • First ligation of RBS to C0051 failed
  • Bands on gels are not valid, too much inconsistency within the data (eg. bands of same parts cut the same were different from each other)

ON switch
  • The following parts were digested: E1010, I13453, J23119, C0051, I12006 with EcoRI & SpeI
  • The following parts were digested: B0034, F1610 with EcoRI & XbaI
  • Ran gel and extracted, but bands failed to appear
  • Restarted digestions according to new assembly plan
We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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