BIOTEC Dresden/Notepad/1 September 2010
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+ | Ligation of following parts with 6e (RBS) | ||
+ | |||
+ | 4a, 14f, 20f, 21f | ||
+ | |||
+ | The ligation mix was then transformed by electroporation and plated and kept for overnight incubation. | ||
'''Fusion Protein''' | '''Fusion Protein''' | ||
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion. | A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion. | ||
- | + | [[Category:BIOTEC Dresden/Notepad/September|September]] | |
- | + | ||
- | + | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} |
Latest revision as of 22:25, 27 October 2010
Ligation of following parts with 6e (RBS)
4a, 14f, 20f, 21f
The ligation mix was then transformed by electroporation and plated and kept for overnight incubation.
Fusion Protein
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.
July |
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