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Team:USTC/Notebook/Meetings/3 August 2010
From 2010.igem.org
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Restriction Enzyme Cutting of pduTUV and PSB1C3 with the mutation | Restriction Enzyme Cutting of pduTUV and PSB1C3 with the mutation | ||
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| + | Concentration determination of product of Restriction Enzyme Cutting of pduTUV and PSB1C3 with the mutation | ||
| + | --------------------------------------------------------------------------------------------------- | ||
| + | gpw： | ||
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| + | PCR:pduJK with the primer of pduJK_f3 and pduJK_r4 under the old standards，Gel extraction and concentration determination | ||
| + | |||
| + | Concentration determination of product of pduJK 1-2，3-4，5-6-PCR | ||
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| + | --------------------------------------------------------------------------------------------------- | ||
Latest revision as of 02:00, 12 September 2010
Evelyn:
PCR: pduJK with the primer of pduJK_f1 and pduJK_r2.
Knightmare:
Colony PCR: pduAB in old standard.
Succeed and send for sequencing
daodao:
Restriction Enzyme Cutting of pduTUV and PSB1C3 with the mutation
Concentration determination of product of Restriction Enzyme Cutting of pduTUV and PSB1C3 with the mutation
gpw：
PCR:pduJK with the primer of pduJK_f3 and pduJK_r4 under the old standards，Gel extraction and concentration determination
Concentration determination of product of pduJK 1-2，3-4，5-6-PCR
