Team:Tokyo Metropolitan/Notebook/Pattern/2010/08/28

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See[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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[[Image:Matsuura_2010-08-28_20hr_02min_(3).JPG]]

Latest revision as of 18:11, 24 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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September 2010
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Contents

2010/08/28

1：PCR

＜Member＞
Mariko, Hitomi


＜Sample＞
・E-coli (having BBa_I732901 plasmid)


＜Protocol＞
See Protocol 2

・Tube(higher temperature/lower temperature in annealing)

  1. Rfp (67.5℃/66.0)
  2. Terminator (66.0/63.0)

Total 4 tubes.


2：Plasmid extraction from E-coli

＜Member＞
Hitomi


＜Sample＞
・transformed E-coli (8/26)

  • BBa_I13521
  • BBa_B0010
  • BBa_B0030
  • BBa_J23110
  • BBa_E0040


＜Protocol＞
SeeProtocol 6


3：DNA concentration measurement

＜Member＞
Hitomi


＜Sample＞
・PCR products


＜Protocol＞

  1. Add 2µl of TE and 2µl of sample into the tube, and mix it gently.
  2. Fall in drops 1 on the measuring machine.


＜Result＞

DNA concentration
concentration A320 A260/A280 A260/A230
RH 565.0 20.24 1.723 1.175
RL 400.5 10.73 1.697 1.462
TH 356.0 8.47 1.622 1.201
TL 324.5 8.31 1.627 1.195


4：Electrophoresis

＜Member＞
Mariko, Hitomi


＜Sample＞
・PCR products


＜Protocol＞
SeeProtocol 8


＜Result＞
Matsuura 2010-08-28 20hr 02min (3).JPG

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