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Revision as of 14:48, 1 September 2010 (view source) LiuChang (Talk | contribs) ← Older edit		Latest revision as of 06:37, 22 September 2010 (view source) Hildegard (Talk | contribs)
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	ligation of pduJ +pduK		ligation of pduJ +pduK
		+
		+	-----
		+
		+	Evelyn:
		+
		+	Restriction Enzyme Cutting of pduN-U and pduN-V.
		+
		+	----
		+
		+	Hilde:
		+
		+	miniprep of pduNTUV
		+
		+	Fastdigest cutting of pduNTUV(Insert,E,S)
		+
		+	Fastdigest cutting of pduABJK(Vector,E,X)
		+
		+	DNA Ligation Kit LONG of pduABJKNTUV (overnight)
		+
		+	SolutionI Ligation of pduABJKNTUV (overnight)

---

Latest revision as of 06:37, 22 September 2010

Jocelyn:

miniprep of pdu J(PSB1C7)

miniprep of pduK(PSB1C7)

---

Zocean:

transformation :

[pduA+midRBS+pduB]

---

LiuChang:

construction of plasmids of the new standard:

ligation of pduN+pduT

ligation of pduU+pduV

ligation of pduJ +pduK

---

Evelyn:

Restriction Enzyme Cutting of pduN-U and pduN-V.

---

Hilde:

miniprep of pduNTUV

Fastdigest cutting of pduNTUV(Insert,E,S)

Fastdigest cutting of pduABJK(Vector,E,X)

DNA Ligation Kit LONG of pduABJKNTUV (overnight)

SolutionI Ligation of pduABJKNTUV (overnight)