Team:USTC/Notebook/Meetings/1 September 2010

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[pduA+midRBS+pduB]
[pduA+midRBS+pduB]
 +
 +
----
 +
 +
LiuChang:
 +
 +
construction of plasmids of the new standard:
 +
 +
ligation of pduN+pduT
 +
 +
ligation of pduU+pduV
 +
 +
ligation of pduJ +pduK
 +
 +
-----
 +
 +
Evelyn:
 +
 +
Restriction Enzyme Cutting of pduN-U and pduN-V.
 +
 +
----
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 +
Hilde:
 +
 +
miniprep of pduNTUV
 +
 +
Fastdigest cutting of pduNTUV(Insert,E,S)
 +
 +
Fastdigest cutting of pduABJK(Vector,E,X)
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 +
DNA Ligation Kit LONG of pduABJKNTUV (overnight)
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 +
SolutionI Ligation of pduABJKNTUV (overnight)

Latest revision as of 06:37, 22 September 2010

Jocelyn:

miniprep of pdu J(PSB1C7)

miniprep of pduK(PSB1C7)

Zocean:

transformation :

[pduA+midRBS+pduB]

LiuChang:

construction of plasmids of the new standard:

ligation of pduN+pduT

ligation of pduU+pduV

ligation of pduJ +pduK

Evelyn:

Restriction Enzyme Cutting of pduN-U and pduN-V.

Hilde:

miniprep of pduNTUV

Fastdigest cutting of pduNTUV(Insert,E,S)

Fastdigest cutting of pduABJK(Vector,E,X)

DNA Ligation Kit LONG of pduABJKNTUV (overnight)

SolutionI Ligation of pduABJKNTUV (overnight)

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