Team:USTC/Notebook/Meetings/1 September 2010

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(New page: Jocelyn: miniprep of pdu J(PSB1C7) miniprep of pduK(PSB1C7))
 
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miniprep of pduK(PSB1C7)
miniprep of pduK(PSB1C7)
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----
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Zocean:
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transformation :
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[pduA+midRBS+pduB]
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 +
----
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LiuChang:
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construction of plasmids of the new standard:
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ligation of pduN+pduT
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ligation of pduU+pduV
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ligation of pduJ +pduK
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-----
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Evelyn:
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Restriction Enzyme Cutting of pduN-U and pduN-V.
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----
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Hilde:
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miniprep of pduNTUV
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Fastdigest cutting of pduNTUV(Insert,E,S)
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Fastdigest cutting of pduABJK(Vector,E,X)
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DNA Ligation Kit LONG of pduABJKNTUV (overnight)
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SolutionI Ligation of pduABJKNTUV (overnight)

Latest revision as of 06:37, 22 September 2010

Jocelyn:

miniprep of pdu J(PSB1C7)

miniprep of pduK(PSB1C7)

Zocean:

transformation :

[pduA+midRBS+pduB]

LiuChang:

construction of plasmids of the new standard:

ligation of pduN+pduT

ligation of pduU+pduV

ligation of pduJ +pduK

Evelyn:

Restriction Enzyme Cutting of pduN-U and pduN-V.

Hilde:

miniprep of pduNTUV

Fastdigest cutting of pduNTUV(Insert,E,S)

Fastdigest cutting of pduABJK(Vector,E,X)

DNA Ligation Kit LONG of pduABJKNTUV (overnight)

SolutionI Ligation of pduABJKNTUV (overnight)

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