Team:Panama

From 2010.igem.org

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Submitted to the Registry</span></a></li>
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Gallery</span></a></li>
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<h3>Republic of Panama</h3>
 +
<p>Situated on the isthmus connecting North and South America,
 +
bordered by Costa Rica to the northwest, Colombia to the southeast,
 +
the Caribbean Sea to the north and the Pacific Ocean to the south.</p>
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<h3>iGEM PANAMA</h3>
 +
<p>In this picture: Carolina, Yisett, Claudio, Nicole, Lorena,
 +
Grimaldo, Natasha, Laura, Ernesto, Dra. Carmenza, Dr. Rao, Ezequiel</p>
 +
</div>
 +
</div>
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<h3>iGEM PANAMA</h3>
 +
<p>In this picture: Yisett, Silke, Yaraví, Carlos, Patrick
 +
Nee.</p>
 +
</div>
 +
</div>
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</h3>
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<img
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<h3>iGEM PANAMA</h3>
 +
<p>In this picture: Leyda, Zeuz, Laura</p>
 +
</div>
 +
</div>
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</h3>
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<h3>iGEM PANAMA</h3>
 +
<p>Labs.</p>
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</div>
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Assembling the enzyme gene Rhamnosiltransferase 1 (rhlAB) into a Biobrick-friendly for rhamnolipid production in E. coli</td>
+
Standardization of the Rhamnosiltransferase 1 gene complex (rhlAB) into
-
              
+
a Biobrick-friendly for rhamnolipid production in <span
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style="font-style: italic;">E. coli</span> </td>
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            <span class="small"></span>abstract</td>
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             <p style="text-align: left;">PROJECT DESCRIPTION</p>
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             <p>Rhamnolipids are naturally occurring glycolipids
-
            <p
+
produced by the <span style="font-style: italic;">Pseudomonas
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style="margin-bottom: 0cm; font-style: normal; line-height: 0.5cm; widows: 2; orphans: 2;"
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aeruginosa</span>. Rhamnolipids have many applications because
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align="justify"><span style="color: rgb(0, 0, 0);"><span
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of their natural biosurfactant, emulsifying, fungicidal, antibiotic
-
style="font-family: sans-serif;"><span
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and pharmaceutical properties. Rhamnolipids can also operate in extreme
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style="font-size: 0.4cm;">Rhamnolipids are naturally occurring glycolipids produced by the <span style="font-style: italic;"> Pseudomonas aeruginosa</span>. Rhamnolipids have many applications, because of their natural biosurfactant, emulsifying, fungicidal, antibiotic, and pharmaceutical properties. Also rhamnolipids can operate in extreme conditions such as high temperature, pH, and salinity. They have a low toxicity, and they are biodegradable compared to their synthetic chemical counterparts. Rhamnolipid is composed of rhamnose sugar molecule and b-hydroxyalkanoic acid. The rhamnosyltransferase 1 complex (RhlAB) is the key enzyme responsible for transferring the rhamnose moiety to the b-hydroxyalkanoic acid moiety to biosynthesize rhamnolipid.  Since the organisms known to produce rhamnolipids are almost exclusively pathogens. For this reason, we propose the non-pathogenic nature of <span style="font-style: italic;">E. coli</span> as an attractive candidate for commercial rhamnolipid production by assembling our rhlAB gene into a BioBrick using synthetic biology to engineer these new rhlAB producing bacteria for human benefit.</span></span></span></span></p>
+
conditions such as high temperature, pH and salinity. Rhamnolipids have
-
 
+
low
-
 
+
toxicity and are biodegradable compared to their synthetic
-
<p><span
+
chemical counterparts. A rhamnolipid is composed of a rhamnose sugar
-
style="font-size: 0.4cm;">Our aim is to construct a BioBrick that leads to the production of rhlAB enzyme complex in <span style="font-style: italic;">E.coli</span>. To accomplish this we must modify the natural gene sequence of rhlAB complex because it includes illegal restriction sites and requires modifications to be fit into a BioBrick. We will perform silent mutations in the gene sequence with a form of mutagenesis. To measure our success we will do bio-assays with our new <span style="font-style: italic;">E. coli rhlAB</span> strain to degrade rhamnose sugars and fatty-acids substrates and turn it in rhamnolipids. We hope that our BioBricks will become a useful tool for the bioengineered industry and widely used as a simple and effective means of rhamnolipid production.</span></p>
+
molecule and a b-hydroxyalkanoic acid. The rhamnolipids are almost
-
<p></p>
+
exclusively produced by pathogenic microorganisms. For this reason we
-
<p></p>
+
propose the non-pathogenic
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<p
+
nature of <span style="font-style: italic;">E.coli</span>
-
  style="text-align: center;margin-bottom: 0cm; font-style: normal; line-height: 0.5cm; widows: 2; orphans: 2;"
+
as an attractive candidate for commercial rhamnolipid production by
-
align="central"><span style="color: rgb(0, 0, 0);"><span
+
assembling our rhlAB gene into a BioBrick using synthetic biology to
-
  style="font-family: sans-serif;"><span
+
engineer this new rhlAB producing bacteria for human benefit.
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National Youth Science Fair </td>
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                  <p>An IGEM Panama presented the fundamentals of synthetic biology to students of colleges of Panama. <span
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Youth Science Fair</span>.<img
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Our team on Panamanian Public TV </td>
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                  <p>Short interview with our team in
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Last Updated on monday, 25 October 2010 21:41 </td>
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alt="PDF"></a></td>
 +
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 +
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onclick="window.open(this.href,'win2','status=no,toolbar=no,scrollbars=yes,titlebar=no,menubar=no,resizable=yes,width=640,height=480,directories=no,location=no'); return false;"
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width="100%"> <a
 +
href="/index.php?option=com_mailto&amp;tmpl=component&amp;link=aHR0cDovL2pvb21sYTE1Mi5waWNram9vbWxhLmNvbS9pbmRleC5waHA/b3B0aW9uPWNvbV9jb250ZW50JnZpZXc9YXJ0aWNsZSZpZD02OndlLWFyZS12b2x1bnRlZXJzJmNhdGlkPTE6bGF0ZXN0LW5ld3MmSXRlbWlkPTUw"
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 +
              <tbody>
 +
                <tr>
 +
                  <td colspan="2" valign="top"
 +
width="70%"> <span class="small"></span></td>
 +
                </tr>
 +
                <tr>
 +
                  <td colspan="2" class="createdate"
 +
valign="top"> Saturday, 02 october 2010 </td>
 +
                </tr>
 +
                <tr>
 +
                  <td colspan="2" valign="top">
 +
                  <p>An article about our team and synthetic
 +
biology was published in today's newspaper <span
 +
style="font-style: italic;">La Prensa</span>.<img
 +
style="width: 160px; height: 107px;" alt="articule"
 +
src="http://congresosectorinformal.com/igem/temp/New_Project/images/LaPrensa-2-10-2010-mini.jpg"></p>
 +
                  </td>
 +
                </tr>
 +
                <tr>
 +
                  <td colspan="2" class="modifydate">
 +
                  </td>
 +
                </tr>
 +
                <tr>
 +
                  <td colspan="2"> <a
 +
href="https://2010.igem.org/Team:Panama/human_practice/laprensa"
 +
class="readon">Read more...</a> </td>
 +
                </tr>
 +
              </tbody>
 +
            </table>
 +
            <span class="article_separator">&nbsp;</span>
 +
            </td>
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            <td class="article_column column_separator"
 +
valign="top" width="50%">
 +
            <table class="contentpaneopen">
 +
              <tbody>
 +
                <tr>
 +
                  <td class="contentheading" width="100%">
 +
Interview with our team on Panamanian Public TV </td>
 +
                  <td class="buttonheading" align="right"
 +
width="100%"> <a
 +
href="/index.php?view=article&amp;catid=1:latest-news&amp;id=44:joomla-security-strike-team&amp;format=pdf"
 +
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 +
onclick="window.open(this.href,'win2','status=no,toolbar=no,scrollbars=yes,titlebar=no,menubar=no,resizable=yes,width=640,height=480,directories=no,location=no'); return false;"
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 +
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 +
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 +
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 +
onclick="window.open(this.href,'win2','width=400,height=350,menubar=yes,resizable=yes'); return false;"><img
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alt="E-mail"></a></td>
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 +
            </table>
 +
            <table class="contentpaneopen">
 +
              <tbody>
 +
                <tr>
 +
                  <td colspan="2" valign="top"
 +
width="70%"> <span class="small"><br>
 +
                  </span>&nbsp;
 +
                  </td>
 +
                </tr>
 +
                <tr>
 +
                  <td colspan="2" class="createdate"
 +
valign="top"> Saturday, 14 September 2010 </td>
 +
                </tr>
 +
                <tr>
 +
                  <td colspan="2" valign="top">
 +
                  <p>Short interview with our team in
 +
SERTV.</p>
 +
                  <p><img style="width: 160px; height: 107px;"
 +
alt="SERTV"
 +
src="http://congresosectorinformal.com/igem/temp/New_Project/images/SERTV-14-09-2010-mini.jpg"></p>
 +
                  </td>
 +
                </tr>
 +
                <tr>
 +
                  <td colspan="2" class="modifydate">
 +
Last Updated on Thursday, 25 October 2010 21:41 </td>
 +
                </tr>
 +
                <tr>
 +
                  <td colspan="2"> <a
 +
href="https://2010.igem.org/wiki/index.php?title=Team:Panama/human_practice/SERTV"
 +
class="readon">Read more...</a> </td>
 +
                </tr>
 +
              </tbody>
 +
            </table>
 +
            <span class="article_separator">&nbsp;</span>
 +
            </td>
 +
          </tr>
 +
        </tbody>
 +
      </table>
       </td>
       </td>
     </tr>
     </tr>
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<!-- End column2 --> </div>
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<!-- Start column1 -->
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<div id="at-col1">
 +
<div class="at-innerpad">
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<dl>
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  <dt class="toggler item1 first"> <span
 +
class="header-l"><span class="header-r">6
 +
-&nbsp;9
 +
October 2010 </span> </span></dt>
 +
  <dd class="content item1 first"> <span
 +
class="article" style="display: block;">
 +
    <p><img
 +
src="http://congresosectorinformal.com/igem/temp/New_Project/images/apanac.png"
 +
alt="dropdown" title="dropdown" align="left"
 +
border="0">Panama's iGEM team presented at the <span
 +
style="font-style: italic;">Thirteenth
 +
National Congress on Science and Technology</span> organized by
 +
APANAC.</p>
 +
    </span> </dd>
 +
  <dt class="toggler item2"> <span class="header-l"><span
 +
class="header-r">17 - 19 october 2010 </span> </span></dt>
 +
  <dd class="content item2"> <span class="article"
 +
style="display: block;">
 +
    <p><img
 +
src="http://congresosectorinformal.com/igem/temp/New_Project/images/ingenio.png"
 +
alt="dropdown" title="dropdown" align="left"
 +
border="0">Panamanian students across the country presented
 +
from Sunday to Tuesday scientific, technological and innovative
 +
projects
 +
at the <span style="font-style: italic;">XIII National
 +
Youth Science Fair</span>.
 +
    </p>
 +
    </span> </dd>
 +
  <dt class="toggler item3"> <span class="header-l"><span
 +
class="header-r">6 - 8 november </span> </span></dt>
 +
  <dd class="content item3"> <span class="article"
 +
style="display: block;">
 +
    <p><img
 +
src="http://congresosectorinformal.com/igem/temp/New_Project/images/jamboree.png"
 +
alt="dropdown" title="dropdown" align="left"
 +
border="0">The iGEM 2010 Jamboree will take place from
 +
November 6th to November 8th, 2010 at MIT in Cambridge, MA.</p>
 +
    </span> </dd>
 +
</dl>
</div>
</div>
-
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  <tbody>
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      <td>
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      <div class="moduletable">
 +
      <h3>Team</h3>
 +
      <p><img
 +
src="http://congresosectorinformal.com/igem/temp/New_Project/images/team-panama.png"
 +
alt="pickjoomla" title="pickjoomla" align="left"
 +
border="0">Our team is composed of 6 students of University
 +
of Panama, 2 students of Technological University of Panama, 5
 +
graduates and 4 PhD advisors.</p>
 +
      </div>
 +
      </td>
 +
      <td>
 +
      <div class="moduletable">
 +
      <h3>Human Practices</h3>
 +
      <p>Understand the responsibilities and issues that arise
 +
with the development of new scientific and technological progress,
 +
especially when these are related to the conception and life
 +
management, as happen with the Synthetic Biology field.</p>
 +
      </div>
 +
      </td>
 +
      <td>
 +
      <div class="moduletable">
 +
      <h3>Donors</h3>
 +
      <p>The
 +
first Panamanian team to participate in
 +
the International Genetically Engineered Machine (iGEM) competition
 +
organized by the Massachussetts Institute of Technology (MIT) needs
 +
your support.</p>
 +
      </div>
 +
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<!-- ///////////// Start Footer ////////////////// -->
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<div>Copyright iGEM PANAMA 2010. All Rights
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Latest revision as of 03:50, 28 October 2010

iGEM Panama

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Republic of Panama

Situated on the isthmus connecting North and South America, bordered by Costa Rica to the northwest, Colombia to the southeast, the Caribbean Sea to the north and the Pacific Ocean to the south.

iGEM PANAMA

In this picture: Carolina, Yisett, Claudio, Nicole, Lorena, Grimaldo, Natasha, Laura, Ernesto, Dra. Carmenza, Dr. Rao, Ezequiel

iGEM PANAMA

In this picture: Yisett, Silke, Yaraví, Carlos, Patrick Nee.

iGEM PANAMA

In this picture: Leyda, Zeuz, Laura

iGEM PANAMA

Labs.

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Standardization of the Rhamnosiltransferase 1 gene complex (rhlAB) into a Biobrick-friendly for rhamnolipid production in E. coli PDF Print E-mail
abstract

Rhamnolipids are naturally occurring glycolipids produced by the Pseudomonas aeruginosa. Rhamnolipids have many applications because of their natural biosurfactant, emulsifying, fungicidal, antibiotic and pharmaceutical properties. Rhamnolipids can also operate in extreme conditions such as high temperature, pH and salinity. Rhamnolipids have low toxicity and are biodegradable compared to their synthetic chemical counterparts. A rhamnolipid is composed of a rhamnose sugar molecule and a b-hydroxyalkanoic acid. The rhamnolipids are almost exclusively produced by pathogenic microorganisms. For this reason we propose the non-pathogenic nature of E.coli as an attractive candidate for commercial rhamnolipid production by assembling our rhlAB gene into a BioBrick using synthetic biology to engineer this new rhlAB producing bacteria for human benefit.

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XIII National Youth Science Fair PDF Print E-mail
Sunday, 17 october 2010

An IGEM Panama presented the fundamentals of synthetic biology to students of colleges of Panama. XIII National Youth Science Fair.articule

Read more...
 
Our team on Panamanian Public TV PDF Print E-mail

 
Saturday, 14 September 2010

Short interview with our team in TV Channel 2.

SERTV

Last Updated on monday, 25 October 2010 21:41
Read more...
 
Article in Panamanian NewsPapers PDF Print E-mail
Saturday, 02 october 2010

An article about our team and synthetic biology was published in today's newspaper La Prensa.articule

Read more...
 
Interview with our team on Panamanian Public TV PDF Print E-mail

 
Saturday, 14 September 2010

Short interview with our team in SERTV.

SERTV

Last Updated on Thursday, 25 October 2010 21:41
Read more...
 

Gold Sponsors


senacytespindicasatespespSyntheticBioConsultingesp
 

Silver Sponsors

Instituto conmemorativo gorgas
espsosaespfundacion


Bronze Sponsors


pedropletteresplibreriaSanMartin
 

Academic Sponsors


UTP

UP

smithsonian

 

6 - 9 October 2010

dropdownPanama's iGEM team presented at the Thirteenth National Congress on Science and Technology organized by APANAC.

17 - 19 october 2010

dropdownPanamanian students across the country presented from Sunday to Tuesday scientific, technological and innovative projects at the XIII National Youth Science Fair.

6 - 8 november

dropdownThe iGEM 2010 Jamboree will take place from November 6th to November 8th, 2010 at MIT in Cambridge, MA.

About Us


TVN
 

SERTV TV Program



TVN 

TVN Channel 2



 TVN

Promotional Video



 TVN

Twitter

Follow igempanama on Twitter

Team

pickjoomlaOur team is composed of 6 students of University of Panama, 2 students of Technological University of Panama, 5 graduates and 4 PhD advisors.

Human Practices

Understand the responsibilities and issues that arise with the development of new scientific and technological progress, especially when these are related to the conception and life management, as happen with the Synthetic Biology field.

Donors

The first Panamanian team to participate in the International Genetically Engineered Machine (iGEM) competition organized by the Massachussetts Institute of Technology (MIT) needs your support.