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Team:USTC/Notebook/Meetings/30 August 2010
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(New page: Transformation of ligation product:newly pduJK PCR product in PSB1C3 vector (XbaI/PstI)%>_<%use the wrong enzyme do it again!... Miniprep of pduJK-5 PCR of pduJ and pduK in new standard....) |
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Transformation of ligation product:newly pduJK PCR product in PSB1C3 vector (XbaI/PstI)%>_<%use the wrong enzyme do it again!... | Transformation of ligation product:newly pduJK PCR product in PSB1C3 vector (XbaI/PstI)%>_<%use the wrong enzyme do it again!... | ||
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PCR of pduJ and pduK in new standard.=> Restriction Enzyme Cutting.=> [in Aug.31st]ligation with PSB1C7. (earI)=> Transformation | PCR of pduJ and pduK in new standard.=> Restriction Enzyme Cutting.=> [in Aug.31st]ligation with PSB1C7. (earI)=> Transformation | ||
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| + | PCR: pdu T, pdu U and pdu V in new standard. | ||
Latest revision as of 00:53, 5 September 2010
Jocelyn:
Transformation of ligation product:newly pduJK PCR product in PSB1C3 vector (XbaI/PstI)%>_<%use the wrong enzyme do it again!...
Miniprep of pduJK-5
PCR of pduJ and pduK in new standard.=> Restriction Enzyme Cutting.=> [in Aug.31st]ligation with PSB1C7. (earI)=> Transformation
The end...
Evelyn:
PCR: pdu T, pdu U and pdu V in new standard.
