"
Team:Calgary/13 September 2010
From 2010.igem.org
m (New page: {{CalgaryNotebookTemplate| '''Monday September 13, 2010'''| Placeholder Text }}) |
|||
| (8 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{CalgaryNotebookTemplate| | {{CalgaryNotebookTemplate| | ||
| - | + | Monday September 13, 2010| | |
| - | + | [[Image:09.13.2010-Himika-male31+degp+i13507 rd.jpg|400px|thumb| Himika's restriction digest of DegP system with EcoRI/ SpeI]] | |
| + | |||
| + | [[Image:09.13.2010-Himika-male31+degp+i13504 rd.jpg|400px|thumb| Himika's restriction digest of DegP system with EcoRI/ SpeI]] | ||
| + | |||
| + | <u>Himika</u> | ||
| + | |||
| + | Today I did a restriction digest of all the miniprepped colonies for DegP systems with EcoRI and SpeI. It looked like the construction worked but I will do a PCR of the plasmids before sending them off to sequencing. Today I also set up overnight cultures of MalE31-CpxR-RFP system. I also set up a restriction digest of MalE with XbaI/ PstI which will be constructed and transformed tomorrow with I0500-B0034. | ||
| + | |||
| + | |||
| + | <u>Emily</u> | ||
| + | |||
| + | Today I made overnight cultures of my malE31 colonies that I think might be biobricked and in the AK Vector. I will miniprep these tomorrow and then proceed with a verification restriction digest. Tonight I also helped Chris and Jeremy clean up some things in the lab. | ||
| + | |||
| + | |||
| + | <u>Chris</u> | ||
| + | |||
| + | Today, I helped Emily and Jeremy clean up the fume hood which had been previously contaminated with ethidium bromide. Yeah, that's a bad thing. We also tidied up the lab for the MDSC 408 class that will be sharing the lab with us under the supervision of our laboratory technician Deirdre Lobb. | ||
}} | }} | ||
Latest revision as of 03:54, 17 October 2010
Monday September 13, 2010
Himika
Today I did a restriction digest of all the miniprepped colonies for DegP systems with EcoRI and SpeI. It looked like the construction worked but I will do a PCR of the plasmids before sending them off to sequencing. Today I also set up overnight cultures of MalE31-CpxR-RFP system. I also set up a restriction digest of MalE with XbaI/ PstI which will be constructed and transformed tomorrow with I0500-B0034.
Emily
Today I made overnight cultures of my malE31 colonies that I think might be biobricked and in the AK Vector. I will miniprep these tomorrow and then proceed with a verification restriction digest. Tonight I also helped Chris and Jeremy clean up some things in the lab.
Chris
Today, I helped Emily and Jeremy clean up the fume hood which had been previously contaminated with ethidium bromide. Yeah, that's a bad thing. We also tidied up the lab for the MDSC 408 class that will be sharing the lab with us under the supervision of our laboratory technician Deirdre Lobb.
