"
Team:TU Delft/11 June 2010 content
From 2010.igem.org
(→Lab work) |
(→Strain Characterization) |
||
| Line 59: | Line 59: | ||
* Store the tube at -80ºC | * Store the tube at -80ºC | ||
| - | Now, we have a cell bank ready to | + | Now, we have a cell bank ready to be used!!! |
=Spring Workshop= | =Spring Workshop= | ||
Latest revision as of 16:09, 25 August 2010
Contents |
Lab work
BioBrick stocks
Today we performed a Qiagen Mini-prep plasmid isolation of the vectors we grew overnight. We saved 3 mL of the bacterial cells to make -80 °C stocks.
The following plasmid concentrations were obtained:
| BioBrick | Concentration (ng/μL) |
| pSB3T5 | 266.0 |
| pSB1T3 | 414.0 |
| pSB1C3 | 284.7 |
| pSB3C5 | 329.3 |
| pSB1K3 | 270.1 |
| pSB1A3 | 271.9 |
| I13401 | 232.1 |
Characterization of Anderson RBS sequences
Next to that we also started digesting the first parts we will be characterizing.
Strain Characterization
By Hugo
I prepared the cell bank according to the following procedure:
- Spin 5 ml of the overnight culture
- Add 500 ul of LB medium
- Add 500 ul of 80% glycerol
- Store the tube at -80ºC
Now, we have a cell bank ready to be used!!!
Spring Workshop
Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep.
