Team:INSA-Lyon

From 2010.igem.org

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<div class="colonnedroite" style="text-indent: 20px; background:white; color:#7F5217; margin-bottom: -15px;">
 
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<p><strong>Latest news ...</strong></p>
 
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<div id="corps4" style="border:0px; padding:10px;">
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<h2 style="color: purple;"><b> Welcome on the INSA-Lyon iGEM Wiki !</b></h2>
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<h3> Welcome on the iGEM 2010 INSA-Lyon Wiki !</h3>
 
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<p>We are students, advisors and instructors very motivated. We are involved for the first time in this international competition, but we'll do our best ! Our lab is located in the BioSciences Department of the National Institut of Applied Sciences (INSA) of Lyon. <br><br></p>
 
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[[Image:INSA-Lyon_construction.jpg|center|450px]]
 
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<p>The Wiki is still under construction, so don't be afraid if some texts or colors appear and disappear, this is not magic, we're just testing the html code !</p>
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<p style="text-align:justify ;"><a href="http://www.mozilla.com/en-US/"><img style="width:20px; height:20px;" src="http://icones.eee-pc.fr/icones/firefox3.png" alt="firefox" /></a>  To fully enjoy our wiki, we recommend you to use Firefox as web browser.
 
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<a href="http://www.mozilla.com/en-US/">Get it now !</a><br><br></p>
 
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<div id="corps3" style="border: 0px solid black; margin-top:10px; padding: 5px;">
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<br><em>Thank you Groningen for sharing your code, you've been most helpful !</em><br><br></p>
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<p style="color:purple;"><b>Our Droppy Project at a glance !</b></p>
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<br />
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<p>The plan below shows the progress of our project. You can click above to obtain more informations.</p><br /><br />
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<SCRIPT LANGUAGE="JavaScript">
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imageNormal=new Image
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imageNormal.src="http://lh4.ggpht.com/_Uc3bmii-yi0/TMbsmzwOVfI/AAAAAAAAAf8/O13Zxwrby30/Droppy_Regulation.PNG"
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imageProduction=new Image
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imageProduction.src="http://lh5.ggpht.com/_Uc3bmii-yi0/TMbsmqOUruI/AAAAAAAAAf4/Q9sfI-LolXw/Droppy_Production.PNG"
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imageUses=new Image
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imageUses.src="http://lh5.ggpht.com/_Uc3bmii-yi0/TMbsmXzlyhI/AAAAAAAAAfw/SNpjrdZ1B0U/Droppy_Insertion.PNG"
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imageRegulation=new Image
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<div id="menug">
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  <ul>
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    <li name="Make a Drop"
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    ONMOUSEOVER="document.image1.src=imageProduction.src"
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    ONMOUSEOUT="document.image1.src=imageNormal.src">
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    <a href="https://2010.igem.org/Team:INSA-Lyon/Project/Stage1/Theory" class="brn"> > Make a Drop </a></li>
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    <li name="Why?"
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    ONMOUSEOVER="document.image1.src=imageUses.src"
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    ONMOUSEOUT="document.image1.src=imageNormal.src">
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    <a href="https://2010.igem.org/Team:INSA-Lyon/Project/Stage2/Theory" class="orange"> > Why ? </a></li>
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    <li name="How"
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    ONMOUSEOVER="document.image1.src=imageRegulation.src"
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    ONMOUSEOUT="document.image1.src=imageNormal.src">
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    <a href="https://2010.igem.org/Team:INSA-Lyon/Project/Stage3/Theory" class="green"> > How ? </a></li>
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<div style="height: 300px;">
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<IMG NAME="image1" SRC="http://lh4.ggpht.com/_Uc3bmii-yi0/TMbsmzwOVfI/AAAAAAAAAf8/O13Zxwrby30/Droppy_Regulation.PNG" alt="Droppy Project"/>
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<div class="colonnedroite">
 
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<tr><td><embed src="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=black&bgcolor=FFFFFF&date_month=11&date_day=05&date_year=0&un=IGEM JAMBOREE 2010&size=normal&mo=11&da=05&yr=2010" type="application/x-shockwave-flash" pluginspage="http://www.macromedia.com/go/getflashplayer" width="200" height="90" wmode="transparent"></embed></div>
 
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<div class="boite5" style="margin-top:-3px; border:2px solid purple;" >
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<p style="color: purple; font-size:1.5em; text-indent:0px; text-align:center;"><font face="Verdana"><i>iGEM Medals requirements</i></font></p><br>
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<p style="font-size:1.3em; text-indent:0px; text-align:left; color:#614e1a;">Bronze</p>
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<a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2010.igem.org/Team:INSA-Lyon" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2010.igem.org/Team:INSA-Lyon" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" />
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG" margin-lfet="5px"/><a href="https://2010.igem.org/Team:INSA-Lyon/Team/Gallery/igem#galleryCheck" style="text-decoration:none; color:black;"> Have fun</a><br>
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<p style="margin-left:-7px"><em>Try to make the biggest spot !</em></p>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/> Judging form<br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/><a href="https://2010.igem.org/Team:INSA-Lyon/Project" style="text-decoration:none; color:black;"> Team's project description</a><br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/> Poster and Talk at Jamboree<br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/><a href="https://2010.igem.org/Team:INSA-Lyon/Parts#partsCheck" style="text-decoration:none; color:black;"> Enter BioBrick's informations</a><br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/> Submit DNA<br>
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<br></p>
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<p style="font-size:1.3em; text-indent:0px; text-align:left;color: #AAAAAA;">Silver</p>
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<h3> About iGEM</h3>
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<p style="text-indent:0px;">
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<IMG SRC="http://lh4.ggpht.com/_Uc3bmii-yi0/TMhTqQbWTSI/AAAAAAAAAoQ/tU6xh9B91iw/caseCocherEmpty.PNG"/> New BioBricks works<br>
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<IMG SRC="http://lh4.ggpht.com/_Uc3bmii-yi0/TMhTqQbWTSI/AAAAAAAAAoQ/tU6xh9B91iw/caseCocherEmpty.PNG"/> Characterize operations of a new BioBrick<br>
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</p>
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<p>Coming soon !</p>
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<p style="font-size:1.3em; text-indent:0px; text-align:left;color: #ffd600;">Gold</p>
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<p style="text-indent:0px;">
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<IMG SRC="http://lh4.ggpht.com/_Uc3bmii-yi0/TMhTqQbWTSI/AAAAAAAAAoQ/tU6xh9B91iw/caseCocherEmpty.PNG"/> Characterize or improve an existing BioBrick<br>
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/><a href="https://2010.igem.org/Team:INSA-Lyon/Acknowledgements#collaboCheck" style="text-decoration:none; color:black;"> Help another iGEM team</a><br>
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<IMG SRC="http://lh4.ggpht.com/_Uc3bmii-yi0/TMhTqQbWTSI/AAAAAAAAAoQ/tU6xh9B91iw/caseCocherEmpty.PNG"/> Develop and document a new technical standard<br>
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<div id="corps3">
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<IMG SRC="http://lh5.ggpht.com/_Uc3bmii-yi0/TMhTZEsQBQI/AAAAAAAAAoI/Wlkf0vghuGk/caseCocherOK.PNG"/><a href="https://2010.igem.org/Team:INSA-Lyon/Safety/Ethics/Medias" style="text-decoration:none; color:black;"> Ethics</a><br>
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<h3>Project Description</h3>
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<p> The great majority of microbes, simply cultivated in their usual media, are able to synthesize considerable amounts of intracellular lipids and store these molecules into large droplets easily stainable and observable. These spherical inclusions are surrounded by a mono-layer of lipids with embedded or attached proteins. To our knowledge, these lipid droplets (or "granules") had never been observed in wild-type <span style="font-style:italic">E. coli</span> strains. But recombinant strains have been constructed by cloning the genes responsible for the production of poly-hydroxybutyrate (PHB), an insoluble polyester, and these molecules were deposited into granules (with the diameter usually ranging between 100 and 500 nm and 5-10 granules per cell). These recombinant bacteria are able to accumulate as much as 80% of their dry weight in PHB.</p>
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<p style="color: purple; font-size:1.5em; text-indent:0px; text-align:center;"><font face="Verdana"> Our Team</font></p><br>
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<p> Considering this very important result, the working hypothesis at the origin of our project is that <span style="font-style:italic">E. coli</span> (and maybe any cell) is able to produce granules when large amounts of hydrophobic molecules are synthesized.</p>
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<img style="margin-left:110px;" class="imagegroupe" src="http://lh3.ggpht.com/_Uc3bmii-yi0/TJDV9cWFgNI/AAAAAAAAACU/nPw9FVMDmLE/DSC01180.JPG" title="group photo" alt="our team" />
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<p> In a first step, we will test this hypothesis by constructing <span style="font-style:italic">E. coli</span> strains which produce other hydrophobic molecules and examining granule synthesis. For this, we intend to construct a new part : a strong promoter sensitive to the shaking speed of the water bath in which the bacteria are cultured. Our objective is to trigger lipid synthesis and granule production just by varying this shaking speed.</p>
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<p> The second step of our project is the use of the PHB granules as a tool to produce and purify recombinant proteins. Various proteins, such as phasins, are attached to the external surface of the granules. Our objective is to construct a part able to target proteins of interest to this surface. Moreover, in order to facilitate purification, the sequence of a self-cleaving protein (intein) will be added to this part.</p>
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<p> In addition, we intend to develop a modelisation project. In plants and bacteria, the reactions involved in fatty acid synthesis are catalyzed by separate monofunctional enzymes. But in animals, evolution has done synthetic biology and the different enzymes are integrated into a single multifunctional polypeptide in which substrates are handed from one functional domain to the next. We have begun to analyse the work of evolution in animals in order to design multifunctional bacterial enzymes that integrate discrete monofunctional enzymes. We hope that these enzymes could promote the synthesis of particular lipids, such as tri-acyl glycerols, and store these molecules into granules.</p>
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<p><br/><br /></p>
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<p style="text-align:center;">Young team from <a href="https://2010.igem.org/Team:INSA-Lyon/Team/Lyon"><font color="black">Lyon</font></a>, and deeply motivated! <br></p><br />
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<p>We are waiting for the Jamboree even if <b>we already got so much from the iGEM project</b>.
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With a team <b>only composed by students</b>, we had to deal with all the scientific and logistic issues and then we acquired great knowledges and competencies. <br></p>
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<p style="text-align:center;"><br>
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Without forgetting the <b>incredible human experience<b> and ...<br><a href="https://2010.igem.org/Team:INSA-Lyon/Team/Gallery/igem"><font color="black">THE FUN</font></a></b> !!</b></p>
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<div class="colonnedroite">
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<p style="text-indent:0; text-align:justify;"><a href="http://www.mozilla.com/en-US/"><img style="width:15px; height:15px;" src="http://icones.eee-pc.fr/icones/firefox3.png" alt="firefox" /></a>  To fully enjoy our wiki, we recommend you to use Firefox as web browser.
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<a href="http://www.mozilla.com/en-US/">Get it now !</a></p>
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<div class="titredroite">
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<p><strong>Countdown</strong></p>
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<!-- Compte à rebours -->
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<tr><td><embed src="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=black&bgcolor=FFFFFF&date_month=11&date_day=05&date_year=0&un=IGEM JAMBOREE 2010&size=normal&mo=11&da=05&yr=2010" type="application/x-shockwave-flash" pluginspage="http://www.macromedia.com/go/getflashplayer" width="200" height="90" wmode="transparent"></embed></div>
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<p><strong>Map of visitors</strong></p>
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<!-- ClustrMaps -->
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<div style="text-align:center; padding:5px;">
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<a href="http://www2.clustrmaps.com/counter/maps.php?url=https://2010.igem.org/Team:INSA-Lyon" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=https://2010.igem.org/Team:INSA-Lyon" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onerror="this.onerror=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com';" />
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</a></div>
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<p style="text-indent: 0px; text-align: center; margin-bottom: 5px;"><em>Try to make the biggest spot !</em></p>
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<a id="csb" href="http://fondation.insa-lyon.fr/"><img style="width: 120px; height: 60px; margin: 10px; padding-top: 15px; padding-bottom: 15px" src="https://static.igem.org/mediawiki/2010/8/81/Team_INSA-Lyon_logo_fondationINSA.jpg"/></a>
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<a id="csb" href="http://www.biomerieux.fr/"><img style="width: 120px; height: 90px; margin: 10px" src="http://www.biomerieux.fr/france/img/logo_home.png"/></a>
<a id="csb" href="http://www.biomerieux.fr/"><img style="width: 120px; height: 90px; margin: 10px" src="http://www.biomerieux.fr/france/img/logo_home.png"/></a>
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<a id="csb" href="http://www.insa-lyon.fr/"><img style="width: 70px; height: 25px; margin: 15px" src="http://www.insa-lyon.fr/sites/default/themes/insa_lyon/img/contenu/logo_bw.gif" /></a>
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<a id="csb" href="http://www.insa-lyon.fr/en"><img style="width: 120px; height: 64.2px; margin: 15px; padding-top: 10px; padding-bottom: 10px" src="https://static.igem.org/mediawiki/2010/4/46/Team_INSA-Lyon_logo_Insa.png" /></a>
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<a id="csb" href="http://www.geneart.com/"><img style="width: 80px; height: 25px; margin: 15px" src="http://www.geneart.com/fileadmin/templates/img/logo.gif"/></a>
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<a id="csb" href="http://mrgene.com/"><img style="width: 70px; height: 40px; margin: 15px" src="http://mrgene.com/portaldata/1/resources/_system/img/logo.gif"/></a>
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<a id="csb" href="http://en.sanofi-aventis.com/home.asp"><img style="width: 120px; height: 110px; margin: 10px" src="http://www.wilogo.com/blog/wp-content/uploads/2009/02/logo_sanofi_aventis.png"/></a>
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<a id="csb" href="http://www.ozyme.fr/"><img style="width: 90px; height: 70px; margin: 10px" src="http://www.crbm.cnrs.fr/IMG/jpg/logo_ozyme2002.JPG" /></a>
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<!-- <a id="csb" href="http://www.rexam.com/index.asp?pageid=1"><img style="width: 70px; height: 25px; margin: 10px" src="http://www.webpackaging.com/files/990371/2077429-ONVCJQJJ/_webpac/images/object/logo.jpg"/></a>
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<a id="csb" href="http://mrgene.com/"><img style="width: 70px; height: 40px; margin: 15px" src="http://mrgene.com/portaldata/1/resources/_system/img/logo.gif"/></a> -->
 
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Latest revision as of 02:49, 28 October 2010




Welcome on the INSA-Lyon iGEM Wiki !

Our Droppy Project at a glance !


The plan below shows the progress of our project. You can click above to obtain more informations.



Droppy Project

iGEM Medals requirements


Bronze

Have fun
Judging form
Team's project description
Poster and Talk at Jamboree
Enter BioBrick's informations
Submit DNA

Silver

New BioBricks works
Characterize operations of a new BioBrick


Gold

Characterize or improve an existing BioBrick
Help another iGEM team
Develop and document a new technical standard
Ethics



Our Team


our team

























Young team from Lyon, and deeply motivated!


We are waiting for the Jamboree even if we already got so much from the iGEM project. With a team only composed by students, we had to deal with all the scientific and logistic issues and then we acquired great knowledges and competencies.


Without forgetting the incredible human experience and ...
THE FUN !!



firefox To fully enjoy our wiki, we recommend you to use Firefox as web browser. Get it now !

Countdown

Map of visitors

Locations of visitors to this page

Try to make the biggest spot !


Retrieved from "http://2010.igem.org/Team:INSA-Lyon"