Team:Kyoto/Notebook

From 2010.igem.org

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== Index ==
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==Notebook==
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===Notebooks===
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* [[Team:Kyoto/Notebook1|Notebook1]]: Construction for Lysisbox.
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* [[Team:Kyoto/Notebook2|Notebook2]]: Measurement of R0011.
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* [[Team:Kyoto/Notebook3|Notebook3]]: Measurement of Lysis Cassette and Lysisbox etc.
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== Notebook ==
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[[#top-section|^Top]]
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===001: Preparing===
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* By: Wataru, Tomo, Yuki, Kazuya, Ken, Makoto
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* Date: 07/20
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* Category: Antibiotic, Parts, Transformation
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* Protocol: [[Team:Kyoto/Protocol#Use_BioBrick_Parts|Use BioBrick Parts]]
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====1. Solubilization fo antibiotics.====
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===Other Information===
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* 1.Make two mixtures as the following.
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* [[Team:Kyoto/Protocols|Protocols]]: Protocols of each experiment such as Polymerase Chain Reaction (PCR), Restriction Digestion, Ligation, Transformation.
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** 1g Ampicillin and 20ml MilliQ (Final concentration 50mg/ml).
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* [[Team:Kyoto/Materials|Materials]]: Strains, DNA, and Primers.
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** 0,5g Ampicillin and 10ml MilliQ (Final concentration 50mg/ml).
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* [[Team:Kyoto/Parts|Parts]]: Construction of each part and BioBrick Parts used in our project.
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* 2.Dispense 1.1ml to 1.5ml tubes.
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* 3.Keep in -20℃ Freezer.
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====2. Make LB plate.====
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[[#top-section|^Top]]
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9 plates for Ampicillin+ and Kanamycin+.
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====3. Transformation of iGEM Parts.====
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{| class="note"
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|Name||Well<sup>*1</sup>||Sample (µl)||Competent Cells (µl)||Total (µl)||Plate||Incubation||Result
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|-
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|<partinfo>J23100</partinfo>||1-18-C||1||20||21||rowspan="7"|LB (Ampicillin+)||rowspan="8"|At 37℃ 7/20 20:50 - 7/21 17:00||○
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|-
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|<partinfo>J23105</partinfo>||1-18-M||1||20||21||○
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|-
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|<partinfo>J23116</partinfo>||1-20-M||1||20||21||○
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|-
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|<partinfo>R0011</partinfo>||1-6-G||1||20||21||○
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|-
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|<partinfo>E0840</partinfo>||1-12-O||1||20||21||○
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|-
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|<partinfo>J06702</partinfo>||2-8-E||1||20||21||○
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|<partinfo>pSB4K5</partinfo>||1-5-G||1||20||21||×
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|-
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|<partinfo>B0015</partinfo>||1-23-L||1||20||21||LB (Kanamycin+)||×
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|}
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* *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.
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====4. Discussion====
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A vector of "pSB4K5" is Kanamycin-resistance, however, we plated it to LB plate (Ampicillin+).
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And We didn't pre-culture "B0015" despite its vector is Kanamycin-resistance.
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So, it was predicted that we will fail the transformation of "pSB4K5" and "B0015".
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Latest revision as of 11:33, 27 October 2010

Contents

Notebook

Notebooks

^Top

Other Information

  • Protocols: Protocols of each experiment such as Polymerase Chain Reaction (PCR), Restriction Digestion, Ligation, Transformation.
  • Materials: Strains, DNA, and Primers.
  • Parts: Construction of each part and BioBrick Parts used in our project.

^Top

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