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| - | {{UT-Tokyo_HEADER}}
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| - | <div id="main_all">
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| | | | |
| - | = '''Sudoku''' =
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| - |
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| - | == '''Protocols and data''' ==
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| - |
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| - | This is an abstract page.
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| - | If you can see detail data, please click each protocols.
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| - | This is an abstract page.
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| - | If you can see detail data, please click each protocols.
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| - |
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| - | == Pre-experiment ==
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| - | [[Image:Sudoku_const_Pre.png|200px|thumb|Pre-experiment]]
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| - |
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| - | === 234 ===
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| - |
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| - | 234
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| - |
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| - | === 0,i,ii,iii ===
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| - |
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| - | 0,i,ii,iii
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| - |
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| - | === Check test ===
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| - |
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| - | Check test
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| - |
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| - |
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| - | {| border="1"
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| - | | Number || Name || Link to BioBrick || Plate coordinate || Vector || Code length
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| - | |-
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| - | | 1 || T7 promoter ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]
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| - | || plate1-6N || pSB1AK8 || 46bp
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| - | |-
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| - | | 2 || rbs ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030]
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| - | || plate1-1H || pSB1A2 || 15bp
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| - | |-
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| - | | 3 || cre recombinase ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J61047 BBa_J61047]
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| - | || plate1-5D || pSB1A2 || 1037bp
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| - | |-
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| - | | 4 || double terminator ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014]
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| - | || plate2-24C || pSB1AK3 || 95bp
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| - | |-
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| - | | 5 || lox66 recombinase site ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I718017 BBa_I718017]
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| - | || plate1-17J || pSB1A2 || 34bp
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| - | |-
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| - | | 6 || Kan resistance (rev) ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J31002 BBa_J31002]
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| - | || plate1-2K || pSB1A2 || 816bp
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| - | |-
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| - | | 7 || rbs (rev) ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014]
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| - | || plate1-1J || pSB1A2 || 15bp
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| - | |-
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| - | | 8 || lox71 recombinase site ||
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| - | order-made
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| - | || --- || --- || 34bp
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| - | |-
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| - | | 9 || single terminator ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B1006 BBa_B1006]
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| - | || plate1-4H || pSB1AK3 || 39bp
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| - | |-
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| - | | 10 || constant express promoter ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119]
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| - | || plate1-18A || pSB1A2 || 35bp
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| - | |-
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| - | | 11 || Tet resistance (rev) ||
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| - | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006 BBa_J31006]
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| - | || plate1-1N || pSB1A2 || 1191bp
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| - | |}
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| - |
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| - |
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| - |
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| - | == Main construct ==
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| - |
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| - | === Research of SP6 RNApol cDNA and F plasmid ===
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| - |
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| - | Research of SP6 RNApol cDNA and F plasmid
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| - |
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| - | === Convert lox71(rev) to lox66(rev) ===
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| - | Convert lox71(rev) to lox66(rev)
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| - |
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| - | === Make Hin(rev) ===
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| - |
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| - | Make Hin(rev)
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| - |
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| - | === Revise MS2 gene ===
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| - |
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| - | Revise MS2 gene
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| - |
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| - | === Make location(rev)-rbs(rev)-location(rev) ===
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| - |
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| - | Make location(rev)-rbs(rev)-location(rev)
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| - |
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| - |
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| - | </div>
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| - | {{UT-Tokyo_FOOTER}}
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