Team:LMU-Munich/Schedule

From 2010.igem.org

(Difference between revisions)
(→PCR and mutagenisis of the digestion sites)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:LMU-Munich/Templates/Page Header}}  
{{:Team:LMU-Munich/Templates/Page Header}}  
-
==PCR and mutagenisis of the digestion sites==
+
==PCR and mutagenesis of the digestion sites==
-
PCR1a: replication of Pdu D with mutagenisis
+
 
 +
 
 +
<b>[[Team:LMU-Munich/Schedule/Primer table|Primer table]] : Primer sequences, intern numeration, Tm</b>
 +
 
 +
 
 +
 
 +
<b>PCR1a: replication of Pdu D with mutagenesis [ ] </b>
[[Image:PCR1aP.jpg]]
[[Image:PCR1aP.jpg]]
-
PCR1b: replication of Pdu D with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]],[[Team:LMU-Munich/Schedule/mpduD_rev| mpduD rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR1b: replication of Pdu D with mutagenesis [ ] </b>
[[Image:PCR1bP.jpg]]
[[Image:PCR1bP.jpg]]
-
PCR2: Joining PCR of PduD
+
Primer used:[[Team:LMU-Munich/Schedule/mpduD_fwd| mpduD fwd]],[[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR2: Joining PCR of PduD [ ] </b>
[[Image:PCR2P.jpg]]
[[Image:PCR2P.jpg]]
-
PCR3a: replication of Pdu C with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]],[[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR3a: replication of Pdu C with mutagenesis [ ] </b>
[[Image:PCR3aP.jpg]]
[[Image:PCR3aP.jpg]]
-
PCR3b: replication of Pdu C with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]],[[Team:LMU-Munich/Schedule/mpduC_rev| mpduC rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR3b: replication of Pdu C with mutagenesis [ ] </b>
[[Image:PCR3bP.jpg]]
[[Image:PCR3bP.jpg]]
-
PCR4: Joining PCR of Pdu C
+
Primer used:[[Team:LMU-Munich/Schedule/mpduC_fwd| mpduC fwd]],[[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR4: Joining PCR of Pdu C [ ] </b>
[[Image:PCR4P.jpg]]
[[Image:PCR4P.jpg]]
-
PCR5: replication of AurF to be joined with Pdu D
+
Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]],[[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR5: replication of AurF to be joined with Pdu D [ ] </b>
[[Image:PCR5P.jpg]]
[[Image:PCR5P.jpg]]
-
PCR6a: replication of LctO with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/5P3AD| 5P3AD]],[[Team:LMU-Munich/Schedule/9P4A| 9P4A]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR6a: replication of LctO with mutagenesis [ ] </b>
[[Image:PCR6aP.jpg]]
[[Image:PCR6aP.jpg]]
-
PCR6b: replication of LctO with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/m1lctO_rev| m1lctO rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR6b: replication of LctO with mutagenesis [ ] </b>
[[Image:PCR6bP.jpg]]
[[Image:PCR6bP.jpg]]
-
PCR7: Joining PCR of LctO
+
Primer used:[[Team:LMU-Munich/Schedule/m1lctO_fwd| m1lctO fwd]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR7: Joining PCR of LctO [ ] </b>
[[Image: PCR7P.jpg]]
[[Image: PCR7P.jpg]]
-
PCR8a: replication of LctO with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR8a: replication of LctO with mutagenesis [ ] </b>
[[Image:PCR8aP.jpg]]
[[Image:PCR8aP.jpg]]
-
PCR8b: replication of LctO with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/m2lctO_rev| m2lctO rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR8b: replication of LctO with mutagenesis [ ] </b>
[[Image:PCR8bP.jpg]]
[[Image:PCR8bP.jpg]]
-
PCR9: Joining PCR of LctO
+
Primer used:[[Team:LMU-Munich/Schedule/m2lctO_fwd| m2lctO fwd]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR9: Joining PCR of LctO [ ] </b>
[[Image:PCR9P.jpg]]
[[Image:PCR9P.jpg]]
-
PCR10: Joining PCR of AurF and PduD
+
Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR10: Joining PCR of AurF and PduD [ ] </b>
[[Image:PCR10P.jpg]]
[[Image:PCR10P.jpg]]
-
PCR11: Joining PCR of LctO and PduC
+
Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]],[[Team:LMU-Munich/Schedule/9P4A| 9P4A]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR11: Joining PCR of LctO and PduC [ ] </b>
[[Image:PCR11P.jpg]]
[[Image:PCR11P.jpg]]
-
PCR12a: replication of the Pdu-Operon with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR12a: replication of the Pdu-Operon with mutagenesis [ ] </b>
[[Image:PCR12aP.jpg]]
[[Image:PCR12aP.jpg]]
-
PCR12b: replication of the Pdu-Operon with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]],[[Team:LMU-Munich/Schedule/mpduA_rev| mpduA rev]]  expected product size:  bp
 +
 
 +
 
 +
<b>PCR12b: replication of the Pdu-Operon with mutagenesis [ ] </b>
[[Image:PCR12bP.jpg]]
[[Image:PCR12bP.jpg]]
-
PCR13: Joining PCR of the Pdu-Operon
+
 
 +
Primer used:[[Team:LMU-Munich/Schedule/mpduA_fwd| mpduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR13: Joining PCR of the Pdu-Operon [ ] </b>
[[Image:PCR131517P.jpg]]
[[Image:PCR131517P.jpg]]
-
PCR14a: replication of the Pdu-Operon with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR14a: replication of the Pdu-Operon with mutagenesis [ ] </b>
[[Image:PCR14aP.jpg]]
[[Image:PCR14aP.jpg]]
-
PCR14b: replication of the Pdu-Operon with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]],[[Team:LMU-Munich/Schedule/mpduB_rev|mpduB rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR14b: replication of the Pdu-Operon with mutagenesis [ ] </b>
[[Image:PCR14bP.jpg]]
[[Image:PCR14bP.jpg]]
-
PCR15: Joining PCR of the Pdu-Operon
+
Primer used:[[Team:LMU-Munich/Schedule/mpduB_fwd| mpduB fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR15: Joining PCR of the Pdu-Operon [ ] </b>
[[Image:PCR131517P.jpg]]
[[Image:PCR131517P.jpg]]
-
PCR16a: replication of the Pdu-Operon with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR16a: replication of the Pdu-Operon with mutagenesis [ ] </b>
[[Image:PCR16aP.jpg]]
[[Image:PCR16aP.jpg]]
-
PCR16b: replication of the Pdu-Operon with mutagenisis
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/mpduJ_rev|mpduJ rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR16b: replication of the Pdu-Operon with mutagenesis [ ] </b>
[[Image:PCR16bP.jpg]]
[[Image:PCR16bP.jpg]]
-
PCR17: Joining PCR of the Pdu-Operon
+
Primer used:[[Team:LMU-Munich/Schedule/mpduJ_fwd|mpduJ fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
 +
 
 +
 
 +
<b>PCR17: Joining PCR of the Pdu-Operon [ ] </b>
[[Image:PCR131517P.jpg]]
[[Image:PCR131517P.jpg]]
-
==In Vitro testing of the chemical kinetics with the enzymes==
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
-
==Transforming the construct in bacteria==
+
 
-
==Testing the system==
+
{{:Team:LMU-Munich/Templates/Page Footer}}
{{:Team:LMU-Munich/Templates/Page Footer}}

Latest revision as of 13:59, 25 October 2010


PCR and mutagenesis of the digestion sites

Primer table : Primer sequences, intern numeration, Tm


PCR1a: replication of Pdu D with mutagenesis [ ] PCR1aP.jpg

Primer used: 1P1D, mpduD rev expected product size: bp


PCR1b: replication of Pdu D with mutagenesis [ ] PCR1bP.jpg

Primer used: mpduD fwd, 3P2D expected product size: bp


PCR2: Joining PCR of PduD [ ] PCR2P.jpg

Primer used: 1P1D, 3P2D expected product size: bp


PCR3a: replication of Pdu C with mutagenesis [ ] PCR3aP.jpg

Primer used: 2P1C, mpduC rev expected product size: bp


PCR3b: replication of Pdu C with mutagenesis [ ] PCR3bP.jpg

Primer used: mpduC fwd, 4P2C expected product size: bp


PCR4: Joining PCR of Pdu C [ ] PCR4P.jpg

Primer used: 2P1C, 4P2C expected product size: bp


PCR5: replication of AurF to be joined with Pdu D [ ] PCR5P.jpg

Primer used: 5P3AD, 9P4A expected product size: bp


PCR6a: replication of LctO with mutagenesis [ ] PCR6aP.jpg

Primer used: 8P3LC, m1lctO rev expected product size: bp


PCR6b: replication of LctO with mutagenesis [ ] PCR6bP.jpg

Primer used: m1lctO fwd, 10P4L expected product size: bp


PCR7: Joining PCR of LctO [ ] PCR7P.jpg

Primer used: 8P3LC, 10P4L expected product size: bp


PCR8a: replication of LctO with mutagenesis [ ] PCR8aP.jpg

Primer used: 8P3LC, m2lctO rev expected product size: bp


PCR8b: replication of LctO with mutagenesis [ ] PCR8bP.jpg

Primer used: m2lctO fwd, 10P4L expected product size: bp


PCR9: Joining PCR of LctO [ ] PCR9P.jpg

Primer used: 8P3LC, 10P4L expected product size: bp


PCR10: Joining PCR of AurF and PduD [ ] PCR10P.jpg

Primer used: 1P1D, 9P4A expected product size: bp


PCR11: Joining PCR of LctO and PduC [ ] PCR11P.jpg

Primer used: 2P1C, 10P4L expected product size: bp


PCR12a: replication of the Pdu-Operon with mutagenesis [ ] PCR12aP.jpg

Primer used: pduA fwd, mpduA rev expected product size: bp


PCR12b: replication of the Pdu-Operon with mutagenesis [ ] PCR12bP.jpg


Primer used: mpduA fwd,pduU rev expected product size: bp


PCR13: Joining PCR of the Pdu-Operon [ ] PCR131517P.jpg

Primer used: pduA fwd,pduU rev expected product size: bp


PCR14a: replication of the Pdu-Operon with mutagenesis [ ] PCR14aP.jpg

Primer used: pduA fwd,mpduB rev expected product size: bp


PCR14b: replication of the Pdu-Operon with mutagenesis [ ] PCR14bP.jpg

Primer used: mpduB fwd,pduU rev expected product size: bp


PCR15: Joining PCR of the Pdu-Operon [ ] PCR131517P.jpg

Primer used:pduA fwd,pduU rev expected product size: bp


PCR16a: replication of the Pdu-Operon with mutagenesis [ ] PCR16aP.jpg

Primer used:pduA fwd,mpduJ rev expected product size: bp


PCR16b: replication of the Pdu-Operon with mutagenesis [ ] PCR16bP.jpg

Primer used:mpduJ fwd,pduU rev expected product size: bp


PCR17: Joining PCR of the Pdu-Operon [ ] PCR131517P.jpg

Primer used:pduA fwd,pduU rev expected product size: bp

 

Retrieved from "http://2010.igem.org/Team:LMU-Munich/Schedule"