Team:Gothenburg-Sweden/test

From 2010.igem.org

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<html>
+
<html xmlns="http://www.w3.org/1999/xhtml">
<head>
<head>
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
<title>Chalmers University of Technology</title>
<title>Chalmers University of Technology</title>
-
<script type="text/javascript" src="http://student.kuleuven.be/~s0173901/wiki/js/jquery.js"></script>
 
<style type="text/css">
<style type="text/css">
-
li {list-style:none; }
 
-
#footer
 
-
{
 
-
    height: 60px;
 
-
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-
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-
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-
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-
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-
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-
    background-image: url(https://static.igem.org/mediawiki/2010/e/e0/Footer.png);
 
-
}
 
-
#footer p{
+
/* wiki modificiations start */
-
    color: #00456e;
+
-
    font-size: 10px;
+
-
    font-weight: normal;
+
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    text-decoration: none;
+
-
padding-left:370px;
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+
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}
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#footer a{
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-
  color: #00456e;
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display:none;
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-
  padding-bottom: 0px;
+
}
}
-
#footer a:hover{
 
-
  text-decoration: underline;
 
-
}
 
-
#accordion {
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-
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display:none;padding:-200px;margin:0;
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padding: 50px 0 0 0;
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color:#ffffff;
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background: #64ABD9;
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-
font-weight: bold;
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margin: 1px;
+
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cursor: pointer;
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+
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      height: 25px;
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list-style: circle;
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-
-webkit-border-radius: 10px;
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-
border-radius: 5px;
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-
}
+
-
#accordion ul {
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+
-
padding: 0 0 0 0;
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}
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#accordion ul li{
+
-
    font-weight:400;
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font-size:12px;
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-
height:20px;
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+
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cursor: auto;
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color:#00456e;
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-
background: url(https://static.igem.org/mediawiki/2010/b/b6/Menu1.gif);
+
-
padding: 0 0 0 20px;
+
-
padding: 5 0 0 30px;
+
-
}
+
-
#accordion a {
+
-
text-decoration: none;
+
-
        color:#00456e;
+
-
      display:block;
+
-
}
+
-
#accordion a:hover {
+
-
+
-
background:  #64ABD9;
+
-
color:#ffffff;
+
-
text-decoration: underline;
+
-
}
+
-
#accordion a:visited {
+
-
color:#00456e;
+
}
}
-
#center{
+
.firstHeading {
-
    width: 616px;
+
display:none;padding:0;margin:0;
-
    padding-left: 15px;
+
-
    float: left;
+
-
+
}
}
-
#center p{
 
-
    padding-left:0px;
 
-
    padding-right:0px;
 
-
    padding-bottom: 10px;
 
 +
#search-controls {
 +
padding-top:10px;
}
}
-
.text{
+
 
-
    padding: 10px;
+
form {
-
    background: #ffffff url(images/text_bg.png);
+
margin: 0px;
-
    background-position: bottom;
+
padding: 0px;
-
    background-repeat: repeat-x;
+
-
    min-height: 64px;
+
}
}
-
#center H1{
+
h2 {background:transparent; text-decoration:none;   }
-
    font-family: Arial, Helvetica, sans-serif;
+
#globalWrapper { background-color: transparent; border: none; margin-bottom: 0px; padding: 0;}
-
    font-weight:normal;
+
#content { background-color: transparent; border: none; padding: 0; margin-bottom: 0px; width: 100%;}
-
    color: #00456e;
+
#bodyContent { border: none; padding:0; margin-bottom:0px; width:100%;}
-
    font-size: 16px;
+
-
    height: 36px;
+
-
    width: 616px;
+
-
    padding-top: 10px;
+
-
text-align:center;
+
-
    background: url(https://static.igem.org/mediawiki/2010/6/64/Title_center.png);
+
-
    background-repeat:no-repeat;
+
-
    background-position: top;
+
-
}
+
-
.read{
+
#top-section { background-color: transparent; height: 20px; margin-top: -30px; margin-left: auto; margin-right: auto; margin-bottom: 0 !important; margin-bottom: 0; padding:0; border: none; }
-
    text-align: right;
+
#p-logo {  display: none;}
-
    padding-bottom:5px;
+
#search-controls { display:none;}
-
    padding-right: 5px;
+
-
}
+
-
#center .read{
+
#menubar.left-menu {width: 400px; display:block; float:left; margin-top:-50px; border: none;}
-
    height: 26px;
+
#menubar.left-menu ul { border: none;}
-
    padding-top: 3px;
+
#menubar.left-menu a{color:#5698b0;}
-
    background: url(images/read.png);
+
#menubar.left-menu a:hover{color:#e6f6fb;}
-
    background-repeat: no-repeat;
+
#menubar.left-menu a:selected{color:#e6f6fb;}
-
    background-position: top;
+
 
-
}
+
#menubar.right-menu {  width:300px; display:block; float:left; margin-top:-50px; border: none;}
 +
#menubar.right-menu ul {  border: none;}
 +
#menubar.right-menu a{color:#5698b0;}
 +
#menubar.right-menu a:hover{color:#e6f6fb;}
-
#center .read a{
+
 
-
    color: #ffffff;
+
/* wiki modifications end/
-
    font-weight:normal;
+
 
-
     font-style: italic;
+
 
 +
 
 +
 
 +
/*to be modified */
 +
 
 +
body {background:url(https://static.igem.org/mediawiki/2010/8/8e/Shadow.png) repeat-y top center;
 +
  padding: 0 0 0 0;
 +
 
 +
    }
 +
a{
 +
     font-family: Arial, Helvetica, sans-serif;
     font-size: 12px;
     font-size: 12px;
-
     padding-right: 15px;
+
     font-weight:bold;
 +
    color: #06639e;
     text-decoration:none;
     text-decoration:none;
-
 
+
     padding:0;
-
}
+
     margin:0;
-
#left{
+
-
    width:180px;
+
-
     padding-left: 67px;
+
-
     padding-top: 25px;
+
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    float: left;
+
-
 
+
}
}
 +
a:hover{
 +
padding:0;margin:0;
 +
    text-decoration: underline;
 +
    color: #000066;
-
#contentSub {
+
-
display:none;
+
}
}
-
#siteSub {
+
a:visited{color:#06639E;}
-
display:none;
+
-
}
+
-
#search-controls {
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*
-
display:none;
+
{
 +
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 +
    margin: 0;
}
}
-
.firstHeading {
 
-
display:none;
 
-
}
 
-
#search-controls {
+
#all{
-
margin-top:30px;
+
  padding:0;
-
}
+
  margin:0;
 +
  padding-left:10%;
 +
  background:  url(https://static.igem.org/mediawiki/2010/3/3a/Bg.png) top center repeat-y;
 +
}  
-
#footer-box {
 
-
display:none;
 
-
}
 
-
body {
 
-
margin: 0px;
 
-
padding: 0px;
 
-
background-color: #FFFFFF;
 
-
}
 
-
form {
+
#header{
-
margin: 0px;
+
  height: 290px;
-
padding: 0px;
+
  background:url(https://static.igem.org/mediawiki/2010/5/50/BannerGo.png) no-repeat;  
-
}
+
  margin-left:15px;
 +
 +
}  
-
h1, h2, h3, h4, h5, h6 {
+
#search {height: 30px;}
-
margin: 0px;
+
#myContent{
-
}
+
margin-top: 0px;
-
body, th, td, input, textarea, select {
 
-
font-family: Arial, Helvetica, sans-serif;
 
-
font-size: 11px;
 
-
color: #333333;
 
}
}
-
p, blockquote, ul, ol, dl {
 
-
margin-top: 0px;
 
-
margin-bottom: 1em;
 
-
text-align: justify;
 
-
}
 
-
a {
+
/* left layer starts */
-
color: #ED8407;
+
-
}
+
-
a:hover {
+
#left{
-
text-decoration: none;
+
    width:138px;
 +
    padding-left:45px;
 +
    float: left;
}
}
-
.style1 {
+
.Title1 {
-
font-size: 10px;
+
    font-family: Comic Sans;
-
color: #A1A1A1;
+
    font-weight:600;
-
height: 15px;
+
    color: #fff;
 +
    font-size: 16px;
 +
    text-align:center;
 +
    width:100%;
 +
    height: 30px;
 +
    padding-top: 7px;
 +
    margin-top:7px;
 +
    background:  url(https://static.igem.org/mediawiki/2010/a/aa/CatTitle.png);
 +
    background-repeat:no-repeat;
 +
    background-position:top left;
}
}
-
.style1 a {
+
.left_grad{
-
color: #ED8407;
+
   
-
text-decoration: none;
+
    background-position: top left;
 +
    padding-top: 0px;
 +
    margin-top: 0px;
}
}
-
.style1 a:hover {
+
.categories{
 +
   
 +
    background: url(https://static.igem.org/mediawiki/2010/1/10/CatCon.png) top repeat-y;
 +
    min-height:100px;
 +
    border: 1px solid #0485B0; 
}
}
-
.list1 {
+
#news {
-
margin-left: 0px;
+
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-
padding-left: 10px;
+
  background: url(https://static.igem.org/mediawiki/2010/1/10/CatCon.png) left top repeat-y;
-
list-style: none;
+
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-
line-height: 15.7px;
+
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-
text-transform: uppercase;
+
-
font-family: Arial, Helvetica, sans-serif;
+
-
font-weight: bold;
+
-
font-size: 11px;
+
}
}
-
 
+
#news p{
-
.list1 li {
+
    color:#07374E;
-
background: url(https://static.igem.org/mediawiki/2010/f/ff/Img4.gif) repeat-x left bottom;
+
    font-weight:normal;
-
padding: 5px 0px 5px 10px;
+
    font-size: 11px;
 +
text-align:left;
 +
    text-decoration:none;
 +
   
 +
    padding: 10px 5px 0px 15px;
 +
 +
   
 +
display:block;
}
}
-
.list1 a {
+
#news a:hover {
-
background: url(https://static.igem.org/mediawiki/2010/1/17/Img7.gif) no-repeat left center;
+
border-bottom: 1px dotted #06639e;
-
text-decoration: none;
+
-
padding-left: 10px;
+
-
color: #A1A1A1;
+
}
}
-
.text1 {
+
.categories a{
-
letter-spacing: -1px;
+
    font-family: Arial, Helvetica, sans-serif;
-
text-transform: uppercase;
+
    font-weight:normal;
-
font-size: 18px;
+
    color: #0485B0;
-
color: #207FF8;
+
    text-decoration: none;
-
+
    text-align:left;
-
}
+
    padding: 4px 0px 4px 20px;
-
.text2 {
+
    font-size:12px;
-
text-transform: uppercase;
+
    padding-top:5px;
-
font-size: 10px;
+
    display:block;
 +
 
}
}
-
.text3 {
+
.categories a:hover{
-
text-transform: uppercase;
+
  text-decoration:none;
-
font-size: 10px;
+
 
-
color: #4A9AFF;
+
  font-size:12px;
 +
  font-weight:700;
 +
  color: #fff;
 +
  background:url(https://static.igem.org/mediawiki/2010/4/47/CatAhover.png);
}
}
-
.text4 {
+
/* left layer ends */
-
letter-spacing: -1px;
+
 
-
text-transform: uppercase;
+
 
-
font-size: 18px;
+
/* center layer starts */
-
color: #ED8407;
+
 
 +
#center{
 +
    width: 600px;
 +
    padding-left: 20px;
 +
    float: left;
 +
}
 +
#center p{
 +
    padding-left:0px;
 +
    padding-right:0px;
 +
    padding-bottom: 10px;
 +
}
}
-
#text5 {
+
    /* menu starts */
-
background: url(https://static.igem.org/mediawiki/2010/6/64/Title_center.png) no-repeat;
+
 
-
text-transform: uppercase;
+
-
        padding-left: 40px;
+
-
        color: #07374E;
+
-
font-size: 14px;
+
-
font-weight: bold;
+
-
        height: 40px;
+
-
        padding-top: 10px;
+
        
        
-
}
+
        #Title2{
 +
 
 +
          height: 40px;
 +
          width: 600px;
 +
       
 +
          background: url(https://static.igem.org/mediawiki/2010/5/50/Title_center1.png);
 +
          background-repeat:no-repeat;
 +
          background-position: center;
 +
          }
-
.text6 {
+
#sddm
-
font-weight: bold;
+
{
-
color: #ED8407;
+
 
-
}
+
  padding-left:62px;
 +
  padding-top: 0px;
 +
}
 +
 +
#sddm li
 +
{     
 +
list-style: none;
 +
float:left;
 +
text-align:center;
 +
}
 +
 +
 +
#sddm li a
 +
 +
{ display: block;
 +
padding: 0px 0px;
 +
width: 96px;
 +
height:33px;
 +
text-align: center;
 +
text-decoration: none;
 +
font-size:  15px;
 +
font-family: Rockwell;
 +
font-weight:normal;
 +
padding-top:10px;
 +
                       
 +
}
 +
#active {
 +
           
 +
}
 +
#active a{
-
.text7 {
+
background: url(https://static.igem.org/mediawiki/2010/0/0c/MActive2.png) top center repeat-y;
-
text-align: center;
+
        color:#FFFFFF;
-
letter-spacing: -2px;
+
font:  13px arial !important;
-
text-transform: uppercase;
+
font-weight:600 !important;
-
font-size: 24px;
+
}
-
color: #FFFFFF;
+
#sddm li a:hover
 +
{ background: url(https://static.igem.org/mediawiki/2010/0/0c/MActive2.png) top center repeat-y;
 +
color:#fff;
 +
font:  13px arial;
 +
font-weight:600;
 +
}
 +
 +
 +
#sddm div
 +
{ position: absolute;
 +
visibility: hidden;
 +
margin-top: -5px;
 +
                     
 +
padding: 0px;
 +
background: #fff ;
 +
border: 1px solid #9CD2ED ;
 +
}
 +
 +
 +
#sddm div a
 +
{ position: relative;
 +
height:16px;
 +
display: block;
 +
margin: 0px;
 +
padding: 4px 20px;
 +
                                padding-top:auto;
 +
                                padding-bottom:auto;
 +
width:auto;
 +
min-width: 100px;
 +
font-family: sans-serif, Tahoma, Verdana, Geneva, Arial, Helvetica;
 +
text-align: left;
 +
text-decoration: none;
 +
background: transparent;
 +
color: #2e6d95;
 +
font-size: 11px ;
 +
border:none;
 +
 +
}
 +
 +
#sddm div a:hover
 +
{ background: #61b5da  url(https://static.igem.org/mediawiki/2010/9/9d/MArrow.png) no-repeat;
 +
 +
color: #FFF;
 +
text-decoration:none;
 +
font:  11px arial;
 +
font-weight:500;
 +
}
 +
 +
/* menu ends */
 +
 +
#text{
 +
    text-align:left;
 +
width:100%;
 +
margin: 0px 10px 10px 20px;
 +
    background: #fff ;
}
}
-
.text8 {
+
#text p
-
text-align: center;
+
{
-
text-transform: none;
+
  padding: 10px 10px 10px 25px;
-
font-size: 12px;
+
  font-family: "Times New Roman";
-
color: #FFFFFF;
+
  font-size:13px;
 +
  line-height:19px;
}
}
-
.bg1 {
+
.logo{
-
background: url(https://static.igem.org/mediawiki/2010/b/bd/Img3.jpg) no-repeat left top;
+
float:left;
-
height: 187px;
+
padding: 10px 20px 10px 20px;
 +
}
 +
 
 +
#text p{color: #07374E;}
 +
 
 +
.read{
 +
    text-align: right;
 +
    padding-bottom:5px;
 +
  /* padding-right: 5px;*/
}
}
-
.bg2 {
+
#myTitle{
-
background: url(https://static.igem.org/mediawiki/2010/1/15/Img4-gbg.gif) repeat-x left top;
+
    font-family: Arial, Helvetica, sans-serif;
 +
    font-weight:normal;
 +
    color: #00456e;
 +
    font-size: 16px;
 +
    height: 36px;
 +
   
 +
    padding-top: 10px;
 +
    padding-right:-200px;
 +
    margin-left: 20px;
 +
    text-align:center;
 +
    background: url(https://static.igem.org/mediawiki/2010/6/64/Title_center.png) left top;
 +
    background-repeat:no-repeat;
 +
 
}
}
-
.bg3 {
+
 
-
background: url(https://static.igem.org/mediawiki/2010/d/da/1-2.jpg) no-repeat left top;
+
#team {
-
height: 117px;
+
padding: 20px 10px 20px 30px;
 +
 
}
}
-
.align-justify { text-align: justify; }
+
/* center ends */
-
.align-right { text-align: right; }
+
-
img { border: none; }
+
/* right starts */
-
.img1 {
+
#right{
-
float: left;
+
    width:160px;
-
margin: 3px 15px 0px 0px;
+
    padding-left: 20px;
 +
    /*padding-right: 135px;*/
 +
    float:left;
}
}
-
.img2 {
+
#right .read a{
-
float: right;
+
   
-
margin: 3px 0px 0px 15px;
+
    color: #06639e;
 +
    font-weight:normal;
 +
    font-style: italic;
 +
    font-size: 12px;
 +
    text-decoration:none;
 +
    border-bottom: 1px #06639e;
 +
    padding-right:10px;
}
}
-
hr { display: none; }
+
#right .read a:hover{border-bottom: 1px dotted #06639e;}
-
.hr1 {
+
 
-
height: 1px;
+
#lab{
-
}
+
-
.STYLE5 {
+
padding-top:0px;
-
font-size: 11px;
+
background: url(https://static.igem.org/mediawiki/2010/1/10/CatCon.png) top left repeat-y;
-
font-weight: normal;
+
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                                            <a href="#">Protein Fusion</a>
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                                            <a href="#">Methods</a>
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                                            <a href="#">Preliminary Results</a>
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     <li><a href="https://2010.igem.org/Team:Gothenburg-Sweden">Home</a></li>
     <li><a href="https://2010.igem.org/Team:Gothenburg-Sweden/Project"  
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                              <p>
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                                <strong>CHALMERS UNIVERSITY OF TECHNOLOGY, GOTHENBURG</strong></p>  
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                              <p>We are a team of 8 students from Chalmers University of Technology    who will represent Gothenburg, SWEDEN in this year’s iGEM competition.    We have started with a promising idea that combines cutting edge    technologies available in the field of Synthetic Biology. Our research    basically aims to constructing an optical reporter mechanism for    cellular stress by tagging the stress activated SNF1 complex in yeast    with fluorescent markers.<br>  
-
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                                  <br>  
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                              The project is performed with two main experimental  pathways: Both  experimental setups will utilize FRET to visualize the  conformational  change that is the result SNF1 activation. The first  approach consists  of fusing fluorescent two proteins, EYFP and ECFP,  with the SNF1  complex. The principal is that when the protein is  activated it  undergoes a conformational change and this should be  indicated by a   change in the FRET-signal. The second approach utilizes  a SAMS-peptide  with fluorescent proteins fused to each end. The  SAMS-peptide will be  phosphorylated by the active SNF1-complex and  undergo a conformational  change that again will be indicated by the  FRET-signal.<br>  
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                                The long term ambition of this project it is to use the  results in  the pharmaceutical industry when performing high-throughput  screening  for new substances or finding the correct drug  concentrations to use.  The yeast cells with the modified SNF1-complex  can be moved through a   micro-fluidic system, gradually exposing them  to an array of substances  or a concentration gradient thereby easily  finding at which  concentration or by what substance the cells are  stressed. <br>  
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                              As of present we have purified the plasmid backbones that  will be  used together with fusion protein insert to transfect the  yeast cells.  The fusion protein primers has arrived and we will start  working with  the fusion PCR as of this week. We have completed the 3D  models of the  fusion proteins and the results look very promising, we  will soon be  able to present docking predictions with the complex.<br>  
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                            Left to right: Per Sunnerhagen, Markus Wilhelmsson, Malin Lindén, Karl Almén Burman, Adnan Kadic, Katarina Risö, Peidi Liu, Goutham Vemuri, Lokeshwaran Manoharan, Kemal Sanli, Julia Fransson </span><br /><br />
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                            <p><strong>What we are doing? </strong><br>
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                            Synthetic readout of cellular stress</p>  
-
     
+
                            <p >The cellular stress is sensed  by a key protein called  AMP-activated  protein kinase (AMPK). The AMPK protein  complex is  conserved among all  eukaryotes, including yeast, plants and humans. </p>
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                <div class="Title3">Lab Notes</div>
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                                          <p>11</p>
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                                        </div>
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                                          <div class="newsright"><a href="#">August 11, 2010</a>
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  <td>&nbsp;</td>
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                                          <p> The PCR reaction from yesterday was checked on a gel. α should be ... </p>
-
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  <td>Please do not hesitate to contact us if you have any questions or  suggestions regarding our project. <br>
+
                                          <div class="read"><a href="#">read more</a></div>
-
We are available at:  igem_goteborg_2010@googlegroups.com</td>
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                                    <p>Göteborgs-Posten, a major daily newspaper in Sweden with the second largest national circulation, published an article involving the interview with our team...
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                        <p> <a href="#">Home</a> | <a href="#"><abbr title="Chalmers University of Technology">CTH</abbr></a> | <a href="#">Sponsors</a> | <a href="#">About Us</a> | <a href="#">Contact Us</a> </p>  
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  <div id="myfooter"> <p>   <a href="#">Home</a> | <a href="#">CTH</a> | <a href="#">Sponsors</a> | <a href="#">About Us</a> | <a href="#">Contact Us</a></p> <p>Chalmers University of Technology, Gothenburg, SWEDEN</p>
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Latest revision as of 12:15, 1 October 2010

Chalmers University of Technology

Team
Introduction CHALMERS Supervisors Students
FUSS
What's all about? Background Protein Fusion Methods Preliminary Results

CHALMERS UNIVERSITY OF TECHNOLOGY, GOTHENBURG

We are a team of 8 students from Chalmers University of Technology who will represent Gothenburg, SWEDEN in this year’s iGEM competition. We have started with a promising idea that combines cutting edge technologies available in the field of Synthetic Biology. Our research basically aims to constructing an optical reporter mechanism for cellular stress by tagging the stress activated SNF1 complex in yeast with fluorescent markers.

The project is performed with two main experimental pathways: Both experimental setups will utilize FRET to visualize the conformational change that is the result SNF1 activation. The first approach consists of fusing fluorescent two proteins, EYFP and ECFP, with the SNF1 complex. The principal is that when the protein is activated it undergoes a conformational change and this should be indicated by a change in the FRET-signal. The second approach utilizes a SAMS-peptide with fluorescent proteins fused to each end. The SAMS-peptide will be phosphorylated by the active SNF1-complex and undergo a conformational change that again will be indicated by the FRET-signal.

The long term ambition of this project it is to use the results in the pharmaceutical industry when performing high-throughput screening for new substances or finding the correct drug concentrations to use. The yeast cells with the modified SNF1-complex can be moved through a micro-fluidic system, gradually exposing them to an array of substances or a concentration gradient thereby easily finding at which concentration or by what substance the cells are stressed.

As of present we have purified the plasmid backbones that will be used together with fusion protein insert to transfect the yeast cells. The fusion protein primers has arrived and we will start working with the fusion PCR as of this week. We have completed the 3D models of the fusion proteins and the results look very promising, we will soon be able to present docking predictions with the complex.

Introduction

Who we are?
Left to right: Per Sunnerhagen, Markus Wilhelmsson, Malin Lindén, Karl Almén Burman, Adnan Kadic, Katarina Risö, Peidi Liu, Goutham Vemuri, Lokeshwaran Manoharan, Kemal Sanli, Julia Fransson

 

What we are doing?
Synthetic readout of cellular stress

The cellular stress is sensed by a key protein called AMP-activated protein kinase (AMPK). The AMPK protein complex is conserved among all eukaryotes, including yeast, plants and humans.

Home | CTH | Sponsors | About Us | Contact Us

Chalmers University of Technology, Gothenburg, SWEDEN

Retrieved from "http://2010.igem.org/Team:Gothenburg-Sweden/test"