Team:UT-Tokyo/Notebook

From 2010.igem.org

(Difference between revisions)
(→Notebook)
 
(2 intermediate revisions not shown)
Line 2: Line 2:
<div id="main_all">
<div id="main_all">
==Notebook==
==Notebook==
 +
 +
We started our experiment on July 10th.
Our experiment notebooks (Japanese) can be read [http://www.igem-ut.net/blog/  here (www.igem-ut.net/blog) ].
Our experiment notebooks (Japanese) can be read [http://www.igem-ut.net/blog/  here (www.igem-ut.net/blog) ].
-
 
+
English Version will come soon!
-
 
+
-
== '''Protocol''' ==
+
-
 
+
-
=== Transformation ===
+
-
 
+
-
-preparation
+
-
・iGEM parts / ligation products
+
-
・LBbroth (No antibiotic)　500uL
+
-
・TE　15uL
+
-
・competent cells
+
-
・plates
+
-
・ice box
+
-
・heat block(42℃)
+
-
 
+
-
-Notice
+
-
competent cells
+
-
→ always onice!!! Melt on ice! Mix DNA as soon as cells melt!
+
-
 
+
-
-Protocol
+
-
iGEMパーツを解凍する場合
+
-
↓P200のピペットマンにチップを付け、チップでフィルムに孔を空ける。
+
-
↓add 15µl TE（MilliQで可）、ピペッティング
+
-
↓1µlをコンピテントセルにいれ30min　on　ice(コンピに対し1/5倍量以下になるように)
+
-
 
+
-
iGEM parts
+
-
 
+
-
Hole film with a tip
+
-
 
+
-
↓Add 15 uL TE or MilliQ, pipetting
+
-
 
+
-
↓Mix 1uL with competent cells 50uL
+
-
 
+
-
↓on ice 30 min
+
-
 
+
-
↓42℃ 45 sec
+
-
 
+
-
↓on ice 10 min
+
-
 
+
-
↓Mix LBbroth 300 uL
+
-
 
+
-
↓37℃ 30 min or room temprature 15 min (amp:omittable)
+
-
 
+
-
↓Plate, 37℃ incubation over night
+
-
 
+
-
 
+
-
 
+
-
 
+
-
Ligation products
+
-
 
+
-
↓Mix ligation products 10uL, competent cells 50uL
+
-
 
+
-
↓on ice 30 min
+
-
 
+
-
↓42℃ 45 sec
+
-
 
+
-
↓on ice 10 min
+
-
 
+
-
↓Mix LBbroth 300 uL
+
-
 
+
-
↓37℃ 30 min or room temprature 15 min (amp:omittable)
+
-
 
+
-
↓Plate, 37℃ incubation over night
+
-
 
+
-
 
+
</div>
</div>
{{UT-Tokyo_FOOTER}}
{{UT-Tokyo_FOOTER}}

Latest revision as of 12:56, 21 August 2010

Notebook

We started our experiment on July 10th.

Our experiment notebooks (Japanese) can be read [http://www.igem-ut.net/blog/ here (www.igem-ut.net/blog) ].

English Version will come soon!

Copyright © 2010 iGEM UT-Tokyo. All rights reserved.

Retrieved from "http://2010.igem.org/Team:UT-Tokyo/Notebook"