According to the guidelines, sequences with 150bp intervening sequences should not be flagged, whereas 200bp and 250bp intervening sequences should be flagged. We obtained pretty good result for 200 and 250 intervening sequences (98% flagged), but results for 150 interveing sequence are less good (86% correct). This means that our software is safe as dangerous sequences may not pass even if hidden, but will produce some false hits.

The 200 intervening sequence and 250 intervening sequence non flagged have pass because of an entry in the Genbank database which have an unconventional name for the select agent, and so not contained in the keyword list. The 150 intervening sequence flagged were flagged because the select agent hidden sequence was prolonged over 200 since the non select agent gene had some part of its sequence near of the one of the select agent. The percent identity of the extended sequence was so very low.

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