Team:Newcastle/Protocol list
From 2010.igem.org
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*[[Team:Newcastle/PCR| PCR]] | *[[Team:Newcastle/PCR| PCR]] | ||
+ | *[[Team:Newcastle/PCR_purification| PCR purification]] | ||
*[[Team:Newcastle/DNA_hydration|DNA Hydration]] | *[[Team:Newcastle/DNA_hydration|DNA Hydration]] | ||
*[[Team:Newcastle/Gibson Cloning|Gibson cloning]] | *[[Team:Newcastle/Gibson Cloning|Gibson cloning]] | ||
*[[Team:Newcastle/E. coli Competence|Preparing competent ''E. coli'']] | *[[Team:Newcastle/E. coli Competence|Preparing competent ''E. coli'']] | ||
*[[Team:Newcastle/Transformation of E. coli|Transformation of ''E. coli'']] | *[[Team:Newcastle/Transformation of E. coli|Transformation of ''E. coli'']] | ||
- | |||
*[[Team:Newcastle/Growing an overnight cultures| Growing an overnight culture]] | *[[Team:Newcastle/Growing an overnight cultures| Growing an overnight culture]] | ||
*[[Team:Newcastle/Qiagen Minipreps|Qiagen Minipreps]] | *[[Team:Newcastle/Qiagen Minipreps|Qiagen Minipreps]] | ||
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*[[Team:Newcastle/Restriction digests| Restriction digests]] | *[[Team:Newcastle/Restriction digests| Restriction digests]] | ||
*[[Team:Newcastle/Gel electrophoresis| Gel electrophoresis]] | *[[Team:Newcastle/Gel electrophoresis| Gel electrophoresis]] | ||
- | *[[Team:Newcastle/ | + | *[[Team:Newcastle/Gel extraction| Gel extraction]] |
+ | *[[TeamNewcastleNanoDrop Spectrophotometer| Nanodrop Spectrophotometer]] | ||
*[[Team:Newcastle/Transformation of B. subtilis| Transformation of ''Bacillus subtilis'']] | *[[Team:Newcastle/Transformation of B. subtilis| Transformation of ''Bacillus subtilis'']] | ||
*[[Team:Newcastle/DNA extraction|DNA extraction of ''Bacillus subtilis'']] | *[[Team:Newcastle/DNA extraction|DNA extraction of ''Bacillus subtilis'']] | ||
*[[Team:Newcastle/Arginine test| Arginine test]] | *[[Team:Newcastle/Arginine test| Arginine test]] | ||
*[[Team:Newcastle/Urease test| Urease test]] | *[[Team:Newcastle/Urease test| Urease test]] | ||
+ | *[[Team:Newcastle/Agar plates| Agar plates]] | ||
+ | *[[Team:Newcastle/Glycerol stocks|Making glycerol stocks]] | ||
+ | *[[Team:Newcastle/Slide Preparation|Slide Preparation]] | ||
+ | *[[Team:Newcastle/Extreme Base Resistance Test|Extreme Base Resistance Test]] | ||
+ | *[[Team:Newcastle/IPTG INduction|Slide preparation for IPTG induced cells]] | ||
+ | *[[Team:Newcastle/Scanning_EM|Scanning Electron Microscopy]] | ||
Latest revision as of 22:03, 27 October 2010
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Our Protocol List
This page contains the many protocols used by Team Newcastle 2010!
- PCR
- PCR purification
- DNA Hydration
- Gibson cloning
- Preparing competent E. coli
- Transformation of E. coli
- Growing an overnight culture
- Qiagen Minipreps
- Ligation
- Restriction digests
- Gel electrophoresis
- Gel extraction
- Nanodrop Spectrophotometer
- Transformation of Bacillus subtilis
- DNA extraction of Bacillus subtilis
- Arginine test
- Urease test
- Agar plates
- Making glycerol stocks
- Slide Preparation
- Extreme Base Resistance Test
- Slide preparation for IPTG induced cells
- Scanning Electron Microscopy
Go back to our main Lab book page