Team:DTU-Denmark

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!align="center" style="background-color:white"|[[Image:DTU-Denmark_logo.png|35px|right]]
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<img src="https://static.igem.org/mediawiki/2010/6/6b/Wiki_banner_967px.png"  align="center"> </img>
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!align="center"|[[Team:DTU-Denmark|Home]]
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!align="center"|[[Team:DTU-Denmark/Team|Team]]
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<title>Welcome to the DTU iGEM wiki!</title>
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=DTU-Denmark Official Team Profile]
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!align="center"|[[Team:DTU-Denmark/Project|Project]]
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!align="center"|[[Team:DTU-Denmark/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:DTU-Denmark/Modeling|Modeling]]
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!align="center"|[[Team:DTU-Denmark/Notebook|Notebook]]
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!align="center"|[[Team:DTU-Denmark/Safety|Safety]]
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|The goal of our project is to enable colonies of E. coli bacteria to transition between production of two different reporter proteins. In our system, switching between states will be induced by exposing the bacteria to light. Each of the states will have a specific frequency associated with it. Such biological “switch” has many potential applications, e.g. easy control of production of additives in industrial biotechnological processes.<br><br>Production of first reporter protein will be induced by red light (660 nm). At the same time, production of the other reporter will be suppressed by a coexpressed repressor. Conversely, production of the second reporter can be induced by blue light (470 nm). Bistability of the system is achieved by using two repressors which negatively regulate each other’s expression. This enables the system to sustain state without continuous input, i. e. once production of a reporter protein is initiated, it will persist until the system is forced into the other state. As a proof of concept, we’re using fluorescent proteins as reporter genes which makes it easy to observe and characterise the system. In principle, however, any reporter gene can be  used.
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|[[Image:DTU_paris.jpg|400px|thumb|right|DTU Team in Paris for the iGEM workshop (Malte is missing)]]
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark" >Home</a></font> </td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Team" >The Team</a> </font></td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Project" >The Project</a> </font></td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Parts" >Parts submitted</a> </font></td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Results">Results</a></font> </td>
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    <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Notebook" title="Day to day lab activity">Notebook</a>
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  <td align="center" ><font face="arial" size="3"><a class="mainLinks" href="https://2010.igem.org/Team:DTU-Denmark/Blog">Blog</a></font> </td>
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  <font color="#990000" face="arial" size="3">
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  <br>
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    Sponsors and acknowledgements<br><br>
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  <font color="DDDDDD" face="arial" size="2">
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<ul>
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    <li><a href="https://2010.igem.org/Team:DTU-Denmark/sponsors" CLASS=leftbar>List of sponsors</a></li>
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    <li><a href="https://2010.igem.org/Team:DTU-Denmark/Acknowledgement" CLASS=leftbar>Acknowledgements and Contributions</a></li>
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    <li><a href="https://2010.igem.org/Team:DTU-Denmark/Collaborations" CLASS=leftbar>Collaborations</a></li>
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<br>
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<font color="#990000" face="arial" size="3">
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<br>   
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Also check out our Danish website<br><br>
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<ul>
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    <li><a href="http://www.bio.dtu.dk/igem.aspx" target="_blank" CLASS=leftbar>DTU iGEM 2010</a></li>
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</ul>
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    <br>
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  </font>
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<font color="#990000" face="arial" size="3">
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<br>   
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Also check us out on:<br><br>
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  </font>
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  <font color="DDDDDD" face="arial" size="2">
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<ul>
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    <li><a href="http://www.facebook.com/pages/iGEM-2010-DTU/134540923224394?ref=ts" target="_blank" CLASS=leftbar>Facebook</a></li>
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    <li><a href="http://twitter.com/igemdtu2010" target="_blank" CLASS=leftbar>Twitter</a></li>
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</ul>
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    <br>
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  </font>
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<font color="#990000" face="arial" size="3">
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<br>
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Our Galllery:<br><br>
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  <font color="DDDDDD" face="arial" size="2">
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    <li><a href="https://2010.igem.org/Team:DTU-Denmark/Gallery">Photo Gallery</a></li>
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<br> 
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<b>DTU DENMARK</b><br><br>
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<p align="justify">The aim of this project is to engineer a genetic bistable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the Salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.</p>
 +
<p align="justify">We present the framework for this development and we characterize the regulatory mechanisms by using fluorescent proteins as outputs. The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the Salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.</p>
 +
<p align="justify">There are multiple potential applications for a biological "switch" like ours in the world of biotechnology i.e. within the medical and environmental field.</p>
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<table class="https://static.igem.org/mediawiki/2010/e/ec/DTU_2biostable.png" align="center">
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<caption align="bottom"><p align="justify"><b>Figure 1</b> Fun in the lab!!</p></caption>
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<table class="https://static.igem.org/mediawiki/2010/9/93/DTU_Corrit_fonden.png" align="center">
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<table class="https://static.igem.org/mediawiki/2010/8/80/DTUlogo.png" align="center">
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<font size="3"><strong>Quick Update</strong></font><br>
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<br>
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<strong>October 24th, 2010</strong><br>
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<p>The structure of our wiki has been changed slightly... Our <b><a href="https://2010.igem.org/Team:DTU-Denmark/Modelling"  target="_blank">Modeling</a></b> section can now be found under <b><a href="https://2010.igem.org/Team:DTU-Denmark/Project"  target="_blank">The Project</a></b>.<br>
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<br>
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</p>
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<strong>October 15th, 2010</strong><br>
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<p>Maya, Thomas and Juliet took the BioLector for a ride! And it was cool way to spend our Friday evening ;D<br>
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<br>
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</p>
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<strong>October 7th, 2010</strong><br>
 +
<p>The DTU-iGEM team will be meeting up with the SDU-iGEM team this Saturday (9th Oct) give each other feedback on our projects and to have a social event!<br>
 +
<br>
 +
</p>
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<strong>October 1st, 2010</strong><br>
 +
<p>Check out our active <a href="https://2010.igem.org/Team:DTU-Denmark/Blog">blog</a>: now with picture of the day!<br>
 +
<br>
 +
</p>
 +
 
 +
<strong>August 14th, 2010</strong><br>
 +
<p>Pictures have been posted so check out our <a href="https://2010.igem.org/Team:DTU-Denmark/Gallery">picture gallery</a>!<br>
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<br>
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Comments or questions to the team? Please <a href="mailto:igem2010@bio.dtu.dk" CLASS=email>Email us</a>
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Latest revision as of 01:52, 28 October 2010

Welcome to the DTU iGEM wiki!

Home The Team The Project Parts submitted Results Notebook Blog

Sponsors and acknowledgements



Also check out our Danish website

Also check us out on:

Our Galllery:
DTU DENMARK

The aim of this project is to engineer a genetic bistable switch that produces two different, mutually exclusive outputs when given two different inputs. The switch is based on the repressor-anti-repressor system of the Salmonella phages Gifsy1 and Gifsy2 and the λ-phage anti-termination system. The latest induced output will remain stable through generations, even once the input ceases, due to the phage regulatory systems.

We present the framework for this development and we characterize the regulatory mechanisms by using fluorescent proteins as outputs. The dynamics of the system will be modeled and we will also attempt to characterize and submit the promoters, repressors and anti-repressors from the Salmonella phages, as well as the two anti-terminator proteins from the lambda phage, as BioBricks.

There are multiple potential applications for a biological "switch" like ours in the world of biotechnology i.e. within the medical and environmental field.

Figure 1 Fun in the lab!!





Quick Update

October 24th, 2010

The structure of our wiki has been changed slightly... Our Modeling section can now be found under The Project.

October 15th, 2010

Maya, Thomas and Juliet took the BioLector for a ride! And it was cool way to spend our Friday evening ;D

October 7th, 2010

The DTU-iGEM team will be meeting up with the SDU-iGEM team this Saturday (9th Oct) give each other feedback on our projects and to have a social event!

October 1st, 2010

Check out our active blog: now with picture of the day!

August 14th, 2010

Pictures have been posted so check out our picture gallery!
Comments or questions to the team? Please

Retrieved from "http://2010.igem.org/Team:DTU-Denmark"