Team:Sheffield/testpage4

From 2010.igem.org

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{{Template:Team:Sheffield/Navigation2}}
{{Template:Team:Sheffield/Navigation2}}
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{{Template:Team:Sheffield/homebox1}}
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<html>
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'''Project Description'''
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<style type="text/css">
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body {
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-x-system-font:none;
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a {
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color:#6587E0;
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margin:0 0px 0;
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a:visited {
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color:#6587E0;
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text-decoration:none;
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a:hover {
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color:#333333;
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text-decoration:underline;
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h1 {
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font-size:32px;
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margin:-18px 10px 0px;
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}
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h2 {
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font-size:26px;
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margin:0 0 10px;
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padding:10px 10px 3px;
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h3 {
 +
font-size:20px;
 +
font-weight:normal;
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padding-left:10px;
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}
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 +
ul {
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margin:0 0 10px;
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padding:0;
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}
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li {
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list-style-type:none;
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}
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img {
 +
border:medium none;
 +
}
 +
 
 +
#profiles {
 +
background: url() repeat-x scroll 0 0;
 +
width: 300px;
 +
        margin: -6px;
 +
top: -100px
 +
position: center;
 +
overflow: auto;
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        background-color: #FFF;
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}
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 +
body {
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-x-system-font:none;
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#profiles2 {
 +
background: url() repeat-x scroll 0 0;
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width: 970px;
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        margin: -6px;
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top: -100px
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position: center;
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overflow: auto;
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        background-color: #FFF;
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}
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#header h1 {
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font-size:90px;
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color:#FFF;
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font-weight:300;
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letter-spacing:-1px;
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padding:22px 0 10px 10px;
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}
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#header h1 a {
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color:#FFFFFF;
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text-decoration:none;
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}
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#header h1 a:hover {
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color:#000000;
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text-decoration:none;
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#header h2 {
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color:#EEEEEE;
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font-size:19px;
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font-weight:100;
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letter-spacing:-1px;
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line-height:12px;
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padding:0 0 0 11px;
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#left {
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background:#727072 none repeat scroll 0 0;
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color:#FFE2EE;
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#paper {
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width: 970px;
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height: 1500px;
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margin: 0px;
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top: -100px
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background:url() no-repeat bottom left;
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position: relative; /* float: left; */
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background-color: #FFF;
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}
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#contentbox_bottom {
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    width: 785px;
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    height: 18px;
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    margin: 10px -20px -21px;
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    background:url() no-repeat top left;
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    position: relative;
 +
  /* float: left; */
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}
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#pb {
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margin:10px -20px 10px;
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background:url() no-repeat scroll 0 0;
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height:60px;
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width: 785px;
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position: relative;
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}
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 +
div.andy {
 +
height: 100px;
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width: 133px;
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margin:0;
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padding:0;
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background-image:url("https://static.igem.org/mediawiki/2010/d/d1/Niilight.jpg");
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}
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div.andy a, div.nav a:link, div.nav a:visited {
 +
display:block;
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}
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 +
div.andy img {
 +
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}
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 +
div.andy a:hover img {
 +
visibility:hidden;
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}
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 +
div.catherine {
 +
height: 100px;
 +
width: 133px;
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margin:0;
 +
padding:0;
 +
background-image:url("https://static.igem.org/mediawiki/2010/0/03/Catherine_5_small.jpg");
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 +
}
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 +
div.catherine a, div.nav a:link, div.nav a:visited {
 +
display:block;
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}
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 +
div.catherine img {
 +
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}
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 +
div.catherine a:hover img {
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visibility:hidden;
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}
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div.qaiser {
 +
height:100px;
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width:133px;
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margin:0;
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padding:0;
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background-image:url("https://static.igem.org/mediawiki/2010/c/cd/Teamqaiser.jpg");
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}
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div.qaiser a, div.nav a:link, div.nav a:visited {
 +
display:block;
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}
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div.qaiser img {
 +
border:0;
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}
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 +
div.qaiser a:hover img {
 +
visibility:hidden;
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}
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 +
div.kate {
 +
height: 100px;
 +
width: 133px;
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margin:0;
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padding:0;
 +
background-image:url("https://static.igem.org/mediawiki/2010/a/a9/Comickate.png");
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}
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div.kate a, div.nav a:link, div.nav a:visited {
 +
display:block;
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}
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 +
div.kate img {
 +
border:0;
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}
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 +
div.kate a:hover img {
 +
visibility:hidden;
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}
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 +
div.nii {
 +
height: 100px;
 +
width: 133px;
 +
margin:0;
 +
padding:0;
 +
background-image:url("https://static.igem.org/mediawiki/2010/5/58/Teamnii.jpg
 +
");
 +
 
 +
}
 +
 
 +
div.nii a, div.nav a:link, div.nav a:visited {
 +
display:block;
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}
 +
 
 +
div.nii img {
 +
border:0;
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}
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 +
div.nii a:hover img {
 +
visibility:hidden;
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}
 +
 
 +
div.matt {
 +
height: 100px;
 +
width: 133px;
 +
margin:0;
 +
padding:0;
 +
background-image:url("https://static.igem.org/mediawiki/2010/0/06/Teammatt.jpg");
 +
 
 +
}
 +
 
 +
div.matt a, div.nav a:link, div.nav a:visited {
 +
display:block;
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}
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 +
div.mattparts img {
 +
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}
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 +
div.matt a:hover img {
 +
visibility:hidden;
 +
}
 +
 
 +
div.steve {
 +
height: 100px;
 +
width: 133px;
 +
margin:0;
 +
padding:0;
 +
background-image:url("https://static.igem.org/mediawiki/2010/c/c9/Teamsteve.jpg");
 +
 
 +
}
 +
 
 +
div.steve a, div.nav a:link, div.nav a:visited {
 +
display:block;
 +
}
 +
 
 +
div.steve img {
 +
border:0;
 +
}
 +
 
 +
div.steve a:hover img {
 +
visibility:hidden;
 +
}
 +
 
 +
div.narmada {
 +
height: 100px;
 +
width: 133px;
 +
margin:0;
 +
padding:0;
 +
background-image:url("https://static.igem.org/mediawiki/2010/1/14/Teamnarmada.jpg");
 +
 
 +
}
 +
 
 +
div.narmada a, div.nav a:link, div.nav a:visited {
 +
display:block;
 +
}
 +
 
 +
div.narmada img {
 +
border:0;
 +
}
 +
 
 +
div.narmada a:hover img {
 +
visibility:hidden;
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}
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 +
div.tom {
 +
height: 100px;
 +
width: 133px;
 +
margin:0;
 +
padding:0;
 +
background-image:url("
 +
https://static.igem.org/mediawiki/2010/6/6c/Teamtom.jpg");
 +
 
 +
}
 +
 
 +
div.tom a, div.nav a:link, div.nav a:visited {
 +
display:block;
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div.tom img {
 +
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div.tom a:hover img {
 +
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 +
 
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div.caz {
 +
height: 100px;
 +
width: 133px;
 +
margin:0;
 +
padding:0;
 +
background-image:url("https://static.igem.org/mediawiki/2010/3/35/Cazlight.jpg");
 +
 
 +
}
 +
 
 +
div.caz a, div.nav a:link, div.nav a:visited {
 +
display:block;
 +
}
 +
 
 +
div.caz img {
 +
border:0;
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}
 +
 
 +
div.caz a:hover img {
 +
visibility:hidden;
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}
 +
 
 +
#content {
 +
    padding: 5px;
 +
    border-left: 1px solid #727072;
 +
    border-right: 1px solid #727072;
 +
    background:#727072 none repeat scroll 0 0;
 +
    position: relative;
 +
  /* float: left; /*
 +
    font-size: 9pt;
 +
    text-align: justify;
 +
}
 +
 
 +
 
 +
 
 +
 
 +
#footer {
 +
border-top:1px solid #CCCCCC;
 +
font-size:11px;
 +
padding:10px 5px;
 +
text-align:left;
 +
}
 +
 
 +
 
 +
 
 +
p {
 +
    margin:5px 0px 10px;
 +
    /* margin:0; */
 +
    /* margin-bottom: 10px; */
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}
 +
.editsection {
 +
    font-size: 75%;
 +
    font-weight: normal;
 +
}
 +
/* This hides the Table of Contents.
 +
#toc {
 +
    display: none;
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}
 +
Not good as disables wikis from all pages.
 +
*/
 +
 
 +
 
 +
</style>
 +
</html>
 +
 
 +
  <div id="profiles" align="center" style="border:none #000"> <html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<title>Template:Navigation</title>
 +
<style type="text/css">
 +
<!--
 +
body {
 +
margin-left: 400px;
 +
margin-top: 0px;
 +
}
 +
-->
 +
</style></head>
 +
 
 +
<body>
 +
<img src="https://static.igem.org/mediawiki/2010/f/f7/Advisors.png" width="300">
 +
<table width="300" border="0" cellspacing="0" cellpadding="0" >
 +
  <tr>
 +
  </tr>
 +
  <tr>
 +
    <td width="100"><div class="andy"><a href="https://2010.igem.org/Team:Sheffield/andy"><img src="https://static.igem.org/mediawiki/2010/e/e1/Barmybalmer_small.jpg" width="100" height="133" border="0" /></a></div></td>
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    <td width="100"><div class="catherine"><a href="https://2010.igem.org/Team:Sheffield/catherine"><img src="https://static.igem.org/mediawiki/2010/0/03/Catherine_5_small.jpg" width="100" height="133" border="0" /></a></div></td>
 +
    <td width="100"><div class="qaiser"><a href="https://2010.igem.org/Team:Sheffield/qaiser"><img src="https://static.igem.org/mediawiki/2010/c/cd/Teamqaiser.jpg" width="100" height="133" border="0" /></a></div></td>
 +
  </tr>
 +
  <tr>
 +
  <tr>
 +
  </table>
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  <div id="profiles2" align="center" style="border:none #000"> <html xmlns="http://www.w3.org/1999/xhtml">
 +
<head>
 +
<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
 +
<title>Template:Navigation</title>
 +
<style type="text/css">
 +
<!--
 +
body {
 +
margin-left: 0px;
 +
margin-top: 0px;
 +
}
 +
-->
 +
</style></head>
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 +
<body>
 +
<img src="https://static.igem.org/mediawiki/2010/1/11/ShefStudents.png" width="700">
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<table width="700" border="0" cellspacing="0" cellpadding="0" >
 +
  <tr>
 +
  </tr>
 +
  <tr>
 +
    <td width="100"><div class="kate"><a href="https://2010.igem.org/Team:Sheffield/kate"><img src="https://static.igem.org/mediawiki/2010/a/a9/Comickate.png" width="100" height="133" border="0" /></a></div></td>
 +
    <td width="100"><div class="nii"><a href="https://2010.igem.org/Team:Sheffield/nii"><img src="https://static.igem.org/mediawiki/2010/5/58/Teamnii.jpg
 +
" width="100" height="133" border="0" /></a></div></td>
 +
    <td><div class="matt"><a href="https://2010.igem.org/Team:Sheffield/matt"><img src="https://static.igem.org/mediawiki/2010/0/06/Teammatt.jpg" width="100" height="133" border="0" /></a></div></td>
 +
    <td><div class="steve"><a href="https://2010.igem.org/Team:Sheffield/steve"><img src="https://static.igem.org/mediawiki/2010/c/c9/Teamsteve.jpg" width="100" height="133" border="0" /></a></div></td>
 +
    <td><div class="tom"><a href="https://2010.igem.org/Team:Sheffield/narmada"><img src="https://static.igem.org/mediawiki/2010/1/14/Teamnarmada.jpg" width="100" height="133" border="0" /></a></div></td>
 +
    <td><div class="tom"><a href="https://2010.igem.org/Team:Sheffield/tom"><img src="
 +
https://static.igem.org/mediawiki/2010/6/6c/Teamtom.jpg" width="100" height="133" border="0" /></a></div></td>
 +
    <td><div class="caz"><a href="https://2010.igem.org/Team:Sheffield/caz"><img src="https://static.igem.org/mediawiki/2010/9/97/Teamcaz.jpg" width="100" height="133" border="0" /></a></div></td>
 +
  </tr>
 +
</table>
 +
</body>
 +
</html>
 +
__NOTOC__</div>
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The aim of our project is to create a bacterium that can detect the presence of vibrio cholerae in water. Some bacteria use quorum-sensing. They produce and release autoinducers; signal molecules which vary in concentration as a function of cell density. When a minimal threshold of autoinducer molecules is detected, gene expression is altered. This provides a mechanism for coordinated behaviour within bacterial populations. The detection of autoinducers involves a histidine kinase in the membrane which detects the signal and autophosporylates on a His residue. A phosphate is then transferred to an aspartate residue of a response regulator which relays the signal. We aim to create a fusion protein in E.coli capable of detecting the presence of CAI-1 (cholera autoinducer). This requires fusion of two related receptors with similar topography. The signal is then transmitted down its native pathway. We have chosen 2 similar receptors to create our fusion protein; BarA and CqsS. BarA is a sensory histidine kinase present in the membrane of E.coli. CqsS is a sensory histidine kinase from vibrio cholerae which detects CAI-1. We aim to fuse the N-terminal (receptor) domain of the CqsS (which detects CAI-1 and autophosphorylates) with the C-terminal (transmitter) domain of BarA (which accepts a phosphate and relays the signal downstream. We will then insert the recombined BarA gene into a high copy plasmid. An alternative is to transform the whole CqsS pathway into E.coli. Phosphorylation of the BarA/CqsS causes the phosphorylation of the response regulator UvrY, which then binds to DNA causing transcription of CsrB (a small regulatory RNA). CsrB inhibits CsrA which, in turn, inhibits certain genes. These genes are part of the pgaABCD operon, a 4 gene locus in E.coli. Upon CAI-1 detection by the fusion kinase, transcription of CsrB causes inhibition of CsrA therefore relieving the inhibition of the pgaABCD gene transcription. We want our biosensor to produce a response visible to the naked eye. We plan to insert E.chromi (from the Cambridge 2009 project) into this operon, this will ensure that its expression is controlled by the activity of our fusion receptor causing a pigment to be produced in the presence of the cholera autoinducer. This project has the potential to be expanded to detect many different pathogens. We have chosen vibrio cholerae as an initial proof of concept as it has a specific autoinducer (CAI-1), reducing the chance of interference in the pathway.
 
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'''Our wiki is currently under construction, but thanks for checking. You can contact us by [mailto:shefigem@gmail.com email]'''
 
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'''Or track our progess on [http://twitter.com/ShefiGEM Twitter]'''</div>
 
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{{Template:Team:Sheffield/homebox1}}
 
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<html><tr>
 
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    <td height="213"></td>
 
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    <td colspan="2" valign="top"><script type="text/javascript" src="http://output84.rssinclude.com/output?type=js&id=132870&hash=6e20fd885d00a73758d7da032eafc1bf"></script></html>
 
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</div>
 
[[Image:Sheffield sponsors.png]]
[[Image:Sheffield sponsors.png]]
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Latest revision as of 15:21, 26 September 2010

Template:Navigation


Template:Navigation
Template:Navigation


Sheffield sponsors.png

Retrieved from "http://2010.igem.org/Team:Sheffield/testpage4"