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Team:UNIPV-Pavia/Calendar/June/settimana2
From 2010.igem.org
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<p align="center"><font size="4"><b>JUNE: WEEK 2</b></font></p></html><hr><br> | <p align="center"><font size="4"><b>JUNE: WEEK 2</b></font></p></html><hr><br> | ||
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==June, 7th== | ==June, 7th== | ||
These BioBrick were resuspended in 15ul ddH20: | These BioBrick were resuspended in 15ul ddH20: | ||
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500 ml LB+Amp was prepared and 21 LB agar plates + Amp were prepared (500 ml). | 500 ml LB+Amp was prepared and 21 LB agar plates + Amp were prepared (500 ml). | ||
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==June, 8th== | ==June, 8th== | ||
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<partinfo>BBa_K165037</partinfo> (grown on LB+Amp) showed only 11 big colonies. | <partinfo>BBa_K165037</partinfo> (grown on LB+Amp) showed only 11 big colonies. | ||
| - | A colony was | + | A colony was picked for each plate and inoculated in 1ml LB+antibiotic. |
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The left culture was re-filled to 5ml with LB+antibiotic and grown overnight at 37°C 200 rpm to be miniprepped tomorrow. | The left culture was re-filled to 5ml with LB+antibiotic and grown overnight at 37°C 200 rpm to be miniprepped tomorrow. | ||
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==June, 9th== | ==June, 9th== | ||
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Ligation of I0, I1, I2 and I3 was performed at 16°C overnight. | Ligation of I0, I1, I2 and I3 was performed at 16°C overnight. | ||
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==June, 10th== | ==June, 10th== | ||
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Plates with transformed ''E.coli'' were incubated overnight at 37°C 220 rpm. | Plates with transformed ''E.coli'' were incubated overnight at 37°C 220 rpm. | ||
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The four plates were named: | The four plates were named: | ||
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and stored at +4°C. | and stored at +4°C. | ||
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==June, 11th== | ==June, 11th== | ||
All four plates grown overnight showed colonies!! | All four plates grown overnight showed colonies!! | ||
| - | Two colonies for each plate were | + | Two colonies for each plate were picked and incubated in 1ml LB+Amp. For I2, a phenotipic test was performed: the same two colonies were inoculated both in LB+Amp and LB+Cm. So, 10 cultures were incubated at 37°C 220 rpm for 8 hours: |
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8 glycerol stocks were prepared and stored at -80°C. Next week a screening will be performed to select positive colonies. | 8 glycerol stocks were prepared and stored at -80°C. Next week a screening will be performed to select positive colonies. | ||
| + | <div align="right"><small>[[#indice|^top]]</small></div> | ||
| + | <!-- table previous next week --> | ||
<br><br> | <br><br> | ||
<table border="0" width="100%" class="menu"> | <table border="0" width="100%" class="menu"> | ||
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</tr> | </tr> | ||
</table> | </table> | ||
| + | <!-- fine table --> | ||
</td> | </td> | ||
<td width="15%" valign="top"> | <td width="15%" valign="top"> | ||
Latest revision as of 12:12, 22 October 2010
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