Team:UNIPV-Pavia/Calendar/June

From 2010.igem.org

(Difference between revisions)

Latest revision as of 13:55, 20 October 2010

JUNE

Finally Bio-Lab activity began!

Select the day you are interested in to find out all the details!

DATE	ACTIVITY
June, 3rd	9° Meeting - Discussion of final and technical details about The Project. Planning of Bio-Lab activity.
JUNE: WEEK 2
June, 7th	1° Bio-Lab - <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in E. coli DH5alpha. Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".
June, 8th	2° Bio-Lab - Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.
June, 9th	3° Bio-Lab - MiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of: I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) I1: <partinfo>BBa_E2050</partinfo> (E-S)+<partinfo>BBa_K165018</partinfo> (E-X) I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
June, 10th	4° Bio-Lab - Transformation of ligations I0, I1, I2, I3 in home made E. coli DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates.
June, 11th	5° Bio-Lab - Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies picked from each plate).
June, 12th	iGEM EU workshop.
June, 13th	iGEM EU workshop.
JUNE: WEEK 3
June, 14th	6° Bio-Lab - Inoculum from glycerol stock of I0-1, I0-2, I1-1, i1-2, I2-1, I3-1 and I3-2 for screening.
June, 15th	7° Bio-Lab - MiniPrep, gel run/cut and ligation of I4: <partinfo>BBa_K165037</partinfo> (S-P) + I1 (X-P) I5: I2 (E-S) + <partinfo>BBa_J61001</partinfo> (E-X) I6: I3 (E-S) + <partinfo>BBa_T9002</partinfo> (E-X)
June, 16th	8° Bio-Lab - Tranformation of I4 (DH5-alpha), I5 (TOP10) and I6 (DH5-alpha).
June, 17th	9° Bio-Lab - Colonies were picked from each plate, I4-1, I4-2, I5-1, I5-2, I6-1, I6-2 and I6-3 were grown in 1ml liquid culture and glycerol stocked (not I5-2, because no growth was observed in LB+Cm and not for I6-1, not grown). Cultures refilled with 5ml LB+antibiotic for tomorrow MiniPrep and screening.
June, 18th	10° Bio-Lab - MiniPrep for I4-1, I4-2, I5-1, I6-2 and I6-3. Screening gel (all the clones are ok!) :)
JUNE: WEEK 4
June, 21st	11° Bio-Lab - Week activities planning and freezer sorting.
June, 22nd	12° Bio-Lab - Inoculations of I6, BBa_J23118, BBa_J23110, BBa_J23114, BBa_J23116.
June, 23rd	13° Bio-Lab - 10° Meeting - Updating about Bio-Lab activity. MiniPrep of ON cultures I6, BBa_J23118, BBa_J23110, BBa_J23114, BBa_J23116, with NanoDrop quantification. Digestions, gel run and extraction of the above. We received BBa_K208001, the Silver-fusion compatible BioBrick part that codes for phasin. Streaked them on two LB+Kan agar plates (named PhaP-1 and PhaP-2)
June, 24th	14° Bio-Lab - Colonies on both PhaP-1 and PhaP-2. Inoculation of one colony per plate, with subsequent growth for 6hrs. Glycerol stocks and refill for tomorrow MiniPrep. Yesterday's ligations heated to inactivate ligase and transformed in TOP10 homemade competent cells.
June, 25th	15° Bio-Lab - Plates all show colonies! Picked two colonies from each plate and incubated in 1ml LB+Amp. The cultures were grown for six hours then glycerol stocks were made. PhaP-1 and 2 MiniPrep and quantification by NanoDrop. DNA quantification was poor, so we decided to perform digestion screening and to repeat Miniprep next week for sequencing. Then they were digested and gel screened, both resulting positive.
JULY: WEEK 1
June, 28th	16° Bio-Lab - Inoculum from glycerol stock for I7-1, I7-2, I8-1, I8-2, I9-1, I9-2, I10-1 and I10-2 in 5ml LB+Amp. Cultures grown overnight. Inoculum of PhaP-1 and PhaP-2 in 5ml LB+Kan and ON growth. Received strains and plasmid from CGSC (Yale University). Details in the week view.
June, 29th	17° Bio-Lab - From I7-1, I7-2, I8-1, I8-2, I9-1, I9-2, I10-1 and I10-2 5ul were aliquoted and diluted in 500ul LB+Amp for a preliminary TECAN test. Then cultures were MiniPrepped. Almost all cultures were digested. PhaP-1 and PhaP-2 were prepared or sequencing. All strains received from Yale Univesity were on blotting paper disks, that were placed on an LB agar plates, resuspended and then streaked. After plate streaking, disks were transferred in falcon tubes containing liquid LB.
June, 30th	18° Bio-Lab - Inoculum of cultures for a TECAN test.

@@ Line 11: / Line 11: @@
 <td valign="top">
 <table border="0" align="center" width="100%" class="trasparenza"><tr><td align="justify" valign="top" style="padding:20px">
+<table class="menu" border="0" width="100%">
+<tr>
+<td align="center">
+[[Team:UNIPV-Pavia/Calendar/June/settimana2|Week 2]]
+</td>
+<td align="center">
+[[Team:UNIPV-Pavia/Calendar/June/settimana3|Week 3]]
+</td>
+<td align="center">
+[[Team:UNIPV-Pavia/Calendar/June/settimana4|Week 4]]
+</td>
+</tr>
+</table>
 <html><p align="center"><font size="4"><b>JUNE</b></font></p></html><hr><br>
-Finally Bio-Lab activity began! Select the day you are interested to find out all the details.<br><br>
+Finally Bio-Lab activity began!<br><br><font size="4"><b><u> Select the day you are interested in to find out all the details!</u></b></font><br><br>
-<table class=cont border="0" width="30%" align="center" valign="top">
+<table class="cont" border="0" width="30%" align="center" valign="top">
 <tr><th align="center" width="12%"><b>DATE</b></th><th align="center"><b>ACTIVITY</b></th></tr>
 <tr><td align="center" valign="top"><html><a name="June_3rd"></a></html>June, 3rd</td><td align="left">9° Meeting - Discussion of final and technical details about [[Team:UNIPV-Pavia/Project|The Project]]. Planning of Bio-Lab activity.</td></tr>
 <tr><th align="center" width="12%" colspan="2"><b>JUNE: WEEK 2</b></th></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 7th|June, 7th]]</td><td align="left"><partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in ''E. coli'' DH5alpha.<br>Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".</td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 7th|June, 7th]]</td><td align="left">1° Bio-Lab - <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in ''E. coli'' DH5alpha.<br>Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".</td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 8th|June, 8th]]</td><td align="left">Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 8th|June, 8th]]</td><td align="left">2° Bio-Lab - Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 9th|June, 9th]]</td><td align="left">MiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of:
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 9th|June, 9th]]</td><td align="left">3° Bio-Lab - MiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of:
 *I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
 *I1: <partinfo>BBa_E2050</partinfo> (E-S)+<partinfo>BBa_K165018</partinfo> (E-X)
 *I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
 *I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 10th|June, 10th]]</td><td align="left">Transformation of ligations I0, I1, I2, I3 in home made ''E. coli'' DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 10th|June, 10th]]</td><td align="left">4° Bio-Lab - Transformation of ligations I0, I1, I2, I3 in home made ''E. coli'' DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates.<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 11th|June, 11th]]</td><td align="left">Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 11th|June, 11th]]</td><td align="left">5° Bio-Lab - Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies picked from each plate).<br></td></tr>
 <tr><td align="center" valign="top"><html><a name="June_12th"></a></html>June, 12th</td><td align="left">iGEM EU workshop.<br></td></tr>
 <tr><td align="center" valign="top"><html><a name="June_13th"></a></html>June, 13th</td><td align="left">iGEM EU workshop.<br></td></tr>
 <tr><th align="center" width="12%" colspan="2"><b>JUNE: WEEK 3</b></th></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 14th|June, 14th]]</td><td align="left">6° Bio-Lab - Lorenzo and Nicolò.<br> Inoculum from glycerol stock of I0-1, I0-2, I1-1, i1-2, I2-1, I3-1 and I3-2 fro screening.</td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 14th|June, 14th]]</td><td align="left">6° Bio-Lab -  Inoculum from glycerol stock of I0-1, I0-2, I1-1, i1-2, I2-1, I3-1 and I3-2 for screening.</td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 15th|June, 15th]]</td><td align="left">7° Bio-Lab - Lorenzo and Manuel L., Sara and Alessandro. MiniPrep, gel run/cut and ligation of
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 15th|June, 15th]]</td><td align="left">7° Bio-Lab - MiniPrep, gel run/cut and ligation of
 *I4: <partinfo>BBa_K165037</partinfo> (S-P) + I1 (X-P)
 *I5: I2 (E-S) + <partinfo>BBa_J61001</partinfo> (E-X)
 *I6: I3 (E-S) + <partinfo>BBa_T9002</partinfo> (E-X)<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 16th|June, 16th]]</td><td align="left">8° Bio-Lab - Susanna Z., Sara and Nicolò. Tranformation of I4 (DH5-alpha), I5 (TOP10) and I6 (DH5-alpha).<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 16th|June, 16th]]</td><td align="left">8° Bio-Lab - Tranformation of I4 (DH5-alpha), I5 (TOP10) and I6 (DH5-alpha).<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 17th|June, 17th]]</td><td align="left">9° Bio-Lab - Susanna Z., Nicolò and Riccardo. Colonies were peaked from each plate, I4-1, I4-2, I5-1, I5-2, I6-1, I6-2 and I6-3 were grown in 1ml liquid culture and glycerol stocked (not I5-2, because no growth was observed in LB+Cm and not for I6-1, not grown). Cultures refilled with 5ml LB+antibiotic for tomorrow MiniPrep and screening.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 17th|June, 17th]]</td><td align="left">9° Bio-Lab - Colonies were picked from each plate, I4-1, I4-2, I5-1, I5-2, I6-1, I6-2 and I6-3 were grown in 1ml liquid culture and glycerol stocked (not I5-2, because no growth was observed in LB+Cm and not for I6-1, not grown). Cultures refilled with 5ml LB+antibiotic for tomorrow MiniPrep and screening.<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 18th|June, 18th]]</td><td align="left">10° Bio-Lab - Susanna Z., Susanna S. and Federica. MiniPrep for I4-1, I4-2, I5-1, I6-2 and I6-3. Screening gel (all the clones are ok!) :)<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 18th|June, 18th]]</td><td align="left">10° Bio-Lab - MiniPrep for I4-1, I4-2, I5-1, I6-2 and I6-3. Screening gel (all the clones are ok!) :)<br></td></tr>
 <tr><th align="center" width="12%" colspan="2"><b>JUNE: WEEK 4</b></th></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 21st|June, 21st]]</td><td align="left">11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 21st|June, 21st]]</td><td align="left">11° Bio-Lab - Week activities planning and freezer sorting.<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 22nd|June, 22nd]]</td><td align="left">12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 22nd|June, 22nd]]</td><td align="left">12° Bio-Lab - Inoculations of I6, BBa_J23118, BBa_J23110, BBa_J23114, BBa_J23116.<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 23rd|June, 23rd]]</td><td align="left">13° Bio-Lab - Sara, Susanna Z., Nicolò.<br>10° Meeting - Updating about Bio-Lab activity.</td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 23rd|June, 23rd]]</td><td align="left">13° Bio-Lab - 10° Meeting - Updating about Bio-Lab activity.<br>MiniPrep of ON cultures I6, BBa_J23118, BBa_J23110, BBa_J23114, BBa_J23116, with NanoDrop quantification. Digestions, gel run and extraction of the above. We received BBa_K208001, the Silver-fusion compatible BioBrick part that codes for phasin. Streaked them on two LB+Kan agar plates (named PhaP-1 and PhaP-2)</td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 24th|June, 24th]]</td><td align="left">14° Bio-Lab - Sara, Susanna Z., Alessandro.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 24th|June, 24th]]</td><td align="left">14° Bio-Lab - Colonies on both PhaP-1 and PhaP-2. Inoculation of one colony per plate, with subsequent growth for 6hrs. Glycerol stocks and refill for tomorrow MiniPrep. Yesterday's ligations heated to inactivate ligase and transformed in TOP10 homemade competent cells.<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 25th|June, 25th]]</td><td align="left">15° Bio-Lab - Susanna Z., Chiara, Riccardo.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 25th|June, 25th]]</td><td align="left">15° Bio-Lab - Plates all show colonies! Picked two colonies from each plate and incubated in 1ml LB+Amp. The cultures were grown for six hours then glycerol stocks were made. PhaP-1 and 2 MiniPrep and quantification by NanoDrop. DNA quantification was poor, so we decided to perform digestion screening and to repeat Miniprep next week for sequencing. Then they were digested and gel screened, both resulting positive. <br></td></tr>
 <tr><th align="center" width="12%" colspan="2"><b>JULY: WEEK 1</b></th></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 28th|June, 28th]]</td><td align="left">16° Bio-Lab - Susanna Z., Nicolò, Federica, Giacomo.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 28th|June, 28th]]</td><td align="left">16° Bio-Lab - Inoculum from glycerol stock for I7-1, I7-2, I8-1, I8-2, I9-1, I9-2, I10-1 and I10-2 in 5ml LB+Amp. Cultures grown overnight. Inoculum of PhaP-1 and PhaP-2 in 5ml LB+Kan and ON growth. Received strains and plasmid from CGSC (Yale University). Details in the week view.<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 29th|June, 29th]]</td><td align="left">17° Bio-Lab - Susanna Z., Nicolò, Riccardo,Giacomo, Lorenzo.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 29th|June, 29th]]</td><td align="left">17° Bio-Lab - From I7-1, I7-2, I8-1, I8-2, I9-1, I9-2, I10-1 and I10-2 5ul were aliquoted and diluted in 500ul LB+Amp for a preliminary TECAN test. Then cultures were MiniPrepped. Almost all cultures were digested. PhaP-1 and PhaP-2 were prepared or sequencing. All strains received from Yale Univesity were on blotting paper disks, that were placed on an LB agar plates, resuspended and then streaked. After plate streaking, disks were transferred in falcon tubes containing liquid LB.<br></td></tr>
-<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 30th|June, 30th]]</td><td align="left">18° Bio-Lab - Susanna Z.<br></td></tr>
+<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 30th|June, 30th]]</td><td align="left">18° Bio-Lab - Inoculum of cultures for a TECAN test.<br></td></tr>
 </table>
 </td>