|
|
| (136 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {{EPFL_2010}} | + | {{EPFL_2010_2}} |
| | <html> | | <html> |
| - | <img src="https://static.igem.org/mediawiki/2010/7/79/EPFL_title_picture_Lab_book.jpg" style="position: relative; top: -87px; left:-5px;"/> | + | |
| | + | <div style="position: absolute; left: 0px; top: 60px; height: 140px; width: 975px;"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/0/0f/EPFL_Title_2_Notebook.png"/></div> |
| | + | |
| | + | <div style="position: absolute; left: 435px; top: 11px; height: 85px; width: 125px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook"> |
| | + | <img alt="" height="80" src="https://static.igem.org/mediawiki/2010/b/b9/EPFL_Menu_2_Notebook.png" width="125" class="auto-style1" /></a></div> |
| | + | |
| | </html> | | </html> |
| | + | |
| | + | {{EPFL_sub_menu_notebook}} |
| | + | |
| | + | |
| | + | <div CLASS="EPFL_content"> |
| | | | |
| | <html> | | <html> |
| - | <head>
| |
| | | | |
| - | <style type="text/css"> | + | <div style="position: absolute; left: 10px; top: 50px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week1"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/b/bd/EPFL_Week01.png"/></a></div> |
| | | | |
| - | .toc {
| + | <div style="position: absolute; left: 220px; top: 60px; height: 200px; width: 170px;"> |
| - | display:none;
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week2"> |
| - | }
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/3/3d/EPFL_Week02.png"/></a></div> |
| | | | |
| - | .EPFL_content {
| + | <div style="position: absolute; left: 430px; top: 45px; height: 200px; width: 170px;"> |
| - | margin-left:0px;
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week3"> |
| - | }
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/c/c9/EPFL_Week03.png"/></a></div> |
| | | | |
| | + | <div style="position: absolute; left: 640px; top: 55px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week4"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/f/fc/EPFL_Week04.png"/></a></div> |
| | | | |
| - | .style1 {
| |
| - | text-align: center;
| |
| - | font-family: "Lucida Sans Unicode";
| |
| - | }
| |
| - | .style2 {
| |
| - | text-align: center;
| |
| - | font-family: "Lucida Sans Unicode";
| |
| - | background-color: #FFD5DC;
| |
| - | }
| |
| | | | |
| - | td.style2:hover, td.style2:hover a{
| |
| - | background-color: #95001B;
| |
| - | color:#FFFFFF;
| |
| - | text-decoration:none;
| |
| - | }
| |
| | | | |
| - | .style2 a{
| |
| - | color:#0000ff;
| |
| - | }
| |
| | | | |
| - | .style3 {
| |
| - | text-align: center;
| |
| - | font-family: "Lucida Sans Unicode";
| |
| - | background-color: #FFA8B7;
| |
| - | }
| |
| - | .style4 {
| |
| - | text-align: center;
| |
| - | font-family: "Lucida Sans Unicode";
| |
| - | color: #FFFFFF;
| |
| - | background-color: #FF5371;
| |
| - | }
| |
| - | </style>
| |
| - | </head>
| |
| - | </html>
| |
| | | | |
| | | | |
| - | <div CLASS="EPFL_content"> | + | <div style="position: absolute; left: 5px; top: 210px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week5"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/1/19/EPFL_Week05.png"/></a></div> |
| | | | |
| - | = How to use the Notebook = | + | <div style="position: absolute; left: 230px; top: 200px; height: 200px; width: 170px;"> |
| - | This is a NOTEbook and not a LABbook. Here you can describe what you have done during your exiting day. If you want to have more details you can just pic up the date and take a look in the IRL Labbook. Think to use sentence, it's a great invention. Use ctrl+F to find something ;)
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week6"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/6/6b/EPFL_Week06.png"/></a></div> |
| | | | |
| - | == template of a day == | + | <div style="position: absolute; left: 440px; top: 215px; height: 200px; width: 170px;"> |
| - | <html> <table cellspacing="15" > <tr> <td> </html> | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week7"> |
| - | For each new day in the lab you must create a new date in the notebook. Please copy-past the following template.
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/4/48/EPFL_Week07.png"/></a></div> |
| | | | |
| - | <pre> | + | <div style="position: absolute; left: 640px; top: 210px; height: 200px; width: 170px;"> |
| - | = 02-08-2010 =
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week8"> |
| - | {| cellspacing="15"
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/2/24/EPFL_Week08.png"/></a></div> |
| - | | style="width: 33.33%" valign="top" |
| + | |
| - | == Other ==
| + | |
| - | It rain a lot today
| + | |
| | | | |
| - | | style="width: 33.33%" valign="top" |
| |
| - | == Asaia ==
| |
| - | Today I speak with an asaia and she answer to me ^^
| |
| | | | |
| - | | style="width: 33.33%" valign="top" |
| |
| - | == Drosophile ==
| |
| - |
| |
| - | |}
| |
| - | </pre>
| |
| | | | |
| - | <html> </td><td> </html>
| |
| | | | |
| - | <u>Here is some explenations : </u>
| |
| | | | |
| - | 1. The date format is important!
| |
| | | | |
| - | 2. Just after the date, put this two lines :
| + | <div style="position: absolute; left: 15px; top: 370px; height: 200px; width: 170px;"> |
| - | <pre> | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week9"> |
| - | {| cellspacing="15"
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/3/34/EPFL_Week09.png"/></a></div> |
| - | | style="width: 33.33%" valign="top" |
| + | |
| - | </pre>
| + | |
| - | 3. Beetween each subtitle, put this line :
| + | |
| - | <pre> | + | |
| - | | style="width: 33.33%" valign="top" |
| + | |
| - | </pre>
| + | |
| - | 4. To end the day just put :
| + | |
| - | <pre> | + | |
| - | |}
| + | |
| - | </pre> | + | |
| - | 5. Of course the text "width:33.33%" must be adapted on how many subtitle you have (for instance 4 subtitles = 25%)
| + | |
| | | | |
| - | <html></td></tr></table></html> | + | <div style="position: absolute; left: 220px; top: 380px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week10"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/c/ca/EPFL_Week10.png"/></a></div> |
| | | | |
| - | = Calendar = | + | <div style="position: absolute; left: 445px; top: 380px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week11"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/8/86/EPFL_Week11.png"/></a></div> |
| | + | <!-- |
| | + | <div style="position: absolute; left: 640px; top: 375px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week12"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/a/a4/EPFL_Week12.png"/></a></div> |
| | | | |
| - | <html>
| |
| - | <table style="width: 100%">
| |
| - | <tr class="style4">
| |
| - | <td>Week</td>
| |
| - | <td>Monday</td>
| |
| - | <td>Tuesday</td>
| |
| - | <td>Wednesday</td>
| |
| - | <td>Thursday</td>
| |
| - | <td>Friday</td>
| |
| - | <td>Satursday</td>
| |
| - | <td>Sunday</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">28</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-07-2010">
| |
| - | 12-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-07-2010">
| |
| - | 13-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-07-2010">
| |
| - | 14-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-07-2010">
| |
| - | 15-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-07-2010">
| |
| - | 16-07-2010</a></td>
| |
| - | <td class="style2">
| |
| | | | |
| - | 17-07-2010</td>
| |
| - | <td class="style2">
| |
| | | | |
| - | 18-07-2010</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">29</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-07-2010">
| |
| - | 19-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-07-2010">
| |
| - | 20-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-07-2010">
| |
| - | 21-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-07-2010">
| |
| - | 22-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-07-2010">
| |
| - | 23-07-2010</a></td>
| |
| - | <td class="style2">
| |
| | | | |
| - | 24-07-2010</td>
| |
| - | <td class="style2">
| |
| | | | |
| - | 25-07-2010</td>
| + | <div style="position: absolute; left: 10px; top: 540px; height: 200px; width: 170px;"> |
| - | </tr>
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook /week13"> |
| - | <tr>
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/8/83/EPFL_Week13.png"/></a></div> |
| - | <td class="style3">30</td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-07-2010">
| + | |
| - | 26-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-07-2010">
| + | |
| - | 27-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook #28-07-2010"> | + | |
| - | 28-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-07-2010">
| + | |
| - | 29-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-07-2010">
| + | |
| - | 30-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| | | | |
| - | 31-07-2010</td>
| + | <div style="position: absolute; left: 220px; top: 545px; height: 200px; width: 170px;"> |
| - | <td class="style2">
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week14"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/e/e5/EPFL_Week14.png"/></a></div> |
| | + | |
| | + | <div style="position: absolute; left: 450px; top: 540px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week15"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/3/3f/EPFL_Week15.png"/></a></div> |
| | + | |
| | + | <div style="position: absolute; left: 650px; top: 540px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week16"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/4/41/EPFL_Week16.png"/></a></div> |
| | + | |
| | + | |
| | + | |
| | + | <div style="position: absolute; left: 370px; top: 680px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week1"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/9/90/EPFL_Week17.png"/></a></div> |
| | + | --> |
| | | | |
| - | 01-08-2010</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">31</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-08-2010">
| |
| - | 02-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-08-2010">
| |
| - | 03-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-08-2010">
| |
| - | 04-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-08-2010">
| |
| - | 05-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-08-2010">
| |
| - | 06-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-08-2010">
| |
| - | 07-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#08-08-2010">
| |
| - | 08-08-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">32</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#09-08-2010">
| |
| - | 09-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#10-08-2010">
| |
| - | 10-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#11-08-2010">
| |
| - | 11-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-08-2010">
| |
| - | 12-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-08-2010">
| |
| - | 13-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-08-2010">
| |
| - | 14-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-08-2010">
| |
| - | 15-08-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">33</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-08-2010">
| |
| - | 16-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#17-08-2010">
| |
| - | 17-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#18-08-2010">
| |
| - | 18-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-08-2010">
| |
| - | 19-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-08-2010">
| |
| - | 20-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-08-2010">
| |
| - | 21-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-08-2010">
| |
| - | 22-08-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">34</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-08-2010">
| |
| - | 23-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#24-08-2010">
| |
| - | 24-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#25-08-2010">
| |
| - | 25-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-08-2010">
| |
| - | 26-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-08-2010">
| |
| - | 27-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-08-2010">
| |
| - | 28-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-08-2010">
| |
| - | 29-08-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">35</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-08-2010">
| |
| - | 30-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#31-08-2010">
| |
| - | 31-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#01-09-2010">
| |
| - | 01-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-09-2010">
| |
| - | 02-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-09-2010">
| |
| - | 03-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-09-2010">
| |
| - | 04-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-09-2010">
| |
| - | 05-09-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">36</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-09-2010">
| |
| - | 06-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-09-2010">
| |
| - | 07-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#08-09-2010">
| |
| - | 08-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#09-09-2010">
| |
| - | 09-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#10-09-2010">
| |
| - | 10-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#11-09-2010">
| |
| - | 11-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-09-2010">
| |
| - | 12-09-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">37</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-09-2010">
| |
| - | 13-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-09-2010">
| |
| - | 14-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-09-2010">
| |
| - | 15-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-09-2010">
| |
| - | 16-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#17-09-2010">
| |
| - | 17-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#18-09-2010">
| |
| - | 18-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-09-2010">
| |
| - | 19-09-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">38</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-09-2010">
| |
| - | 20-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-09-2010">
| |
| - | 21-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-09-2010">
| |
| - | 22-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-09-2010">
| |
| - | 23-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#24-09-2010">
| |
| - | 24-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#25-09-2010">
| |
| - | 25-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-09-2010">
| |
| - | 26-09-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">39</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-09-2010">
| |
| - | 27-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-09-2010">
| |
| - | 28-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-09-2010">
| |
| - | 29-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-09-2010">
| |
| - | 30-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#01-10-2010">
| |
| - | 01-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-10-2010">
| |
| - | 02-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-10-2010">
| |
| - | 03-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">40</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-10-2010">
| |
| - | 04-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-10-2010">
| |
| - | 05-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-10-2010">
| |
| - | 06-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-10-2010">
| |
| - | 07-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#08-10-2010">
| |
| - | 08-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#09-10-2010">
| |
| - | 09-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#10-10-2010">
| |
| - | 10-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">41</td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#11-10-2010">11-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-10-2010">12-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-10-2010">13-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-10-2010">14-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-10-2010">15-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-10-2010">16-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#17-10-2010">17-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">42</td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#18-10-2010">18-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-10-2010">19-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-10-2010">20-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-10-2010">21-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-10-2010">22-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-10-2010">23-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#24-10-2010">24-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">43</td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#25-10-2010">25-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-10-2010">26-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-10-2010">27-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-10-2010">28-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-10-2010">29-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-10-2010">30-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#31-10-2010">31-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">44</td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#01-11-2010">01-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-11-2010">02-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-11-2010">03-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-11-2010">04-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-11-2010">05-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-11-2010">06-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-11-2010">07-11-2010</a></td>
| |
| - | </tr>
| |
| - | </table>
| |
| | </html> | | </html> |
| | | | |
| - | =12-07-2010=
| |
| - | * OD measurement of Asaia’s culture
| |
| - | * Chemical competence for E.Coli DB3.1 (step : Day 3)
| |
| - | * PCR (Asaia ORI + primers)=12-07-2010=
| |
| - | * OD measurement of Asaia’s culture
| |
| - | * Chemical competence for E.Coli DB3.1 (step : Day 3)
| |
| - | * PCR (Asaia ORI + primers)
| |
| - | * Made GLY agar plates without antibiotics -> failed (medium was too old)
| |
| - | * Asaia O/N culture without antibiotics in order to recover some WT
| |
| - | * Text for the sponsor
| |
| - |
| |
| - |
| |
| - | =13-07-2010=
| |
| - | * Chemical competence for E.Coli DB3.1 (step : Day 4) -> stored at -80°C
| |
| - | * Preparation of GLY Medium / LB Medium / SOC Medium / TAE 1X / TAE 0.5X
| |
| - | * Run a gel for the PCR -> failed (mix of two different kits)
| |
| - | * Preparation of plates (GLY Agar / LB+Kan Agar / LB+Amp Agar)
| |
| - | * Learned to autoclave
| |
| - | * Restarted PCR (Asaia ORI + primers)
| |
| - | * Plated Asaia (O/N culture)
| |
| - | * Choice of Antibiotics Biobricks resistance (Kan / Amp / Tet / Chl) from the iGem Kit and preparation of them (streak out)
| |
| - | * Transformation of E.coli DB3.1 with pUC19 to check its competence
| |
| - | * Transformation of E.coli DH5 with p1003 (Kan) / p1002 (Amp) / p1004 (Chl) / p1005 (Tet)
| |
| - | * E.coli DB3.1 and DH5 were plated on LB+Amp Agar plates (Kan was plated on LB+Kan Agar plate)
| |
| - | * Ordered material
| |
| - | * Wiki brainstorming
| |
| - | * Protocols printing and organization
| |
| | | | |
| | | | |
| - | =14-07-2010=
| |
| - | * Run a gel for the PCR of the previous day -> worked
| |
| - | * Purification of PCR’s product -> ok
| |
| - | * Preparation of the BBa_151020 (streak out)
| |
| - | * Competence Calculation of E.Coli DB3.1 (+pUC10, Amp) -> ok
| |
| - | * Look at Asaia with microscope -> we have cells (pictures)
| |
| - | * Growth Curve for Asaia (OD measurement each hour) -> both manually (ok) + with the plate reader
| |
| - | * Plated Asaia to recover some WT (streak out)
| |
| - | * Preparation of Asaia Culture (for Lemaître experiment) with Kan
| |
| - | * Protocol LateX template
| |
| - | * O/N culture of the E.coli DH5 transformed with the BB Antibiotics resistance (4x) -> miniprep
| |
| - | * Learning HTML for the wiki
| |
| - | * Text for the project presentation due to iGem
| |
| - | * Writing Protocols of basic procedures for the wiki page
| |
| - | * Culture of Asaia + Kan
| |
| | | | |
| | | | |
| - | =15-07-2010=
| |
| - | * MaxiPrep of Lemaître’s culture
| |
| - | * Purification of the BB resistances from the E.Coli cultures
| |
| - | * Protocol for cloning the Asaia Vector
| |
| - | * Enzyme digestion of the BB resistances and Asaia_ORI
| |
| - | * Cloning (Asaia_ORI + Vector/ Asaia_ORI + BB + Vector)
| |
| - | * Wiki text
| |
| - | * Preparation of LB agar + Cm
| |
| - | * Electrophoresis of resistance fragments (Works!!!) and extraction
| |
| - | * Purification of extracted DNA from agarose gel
| |
| | | | |
| | | | |
| - | =16-07-2010=
| |
| - | * ligation Asaia origin BB + vector
| |
| - | * ligation Asaia origin BB + Kan + vector
| |
| - | * ligation Asaia origin BB + Amp + vector
| |
| - | * ligation Asaia origin BB + Cm + vector
| |
| - | * ligation Asaia origin BB + Tet + vector
| |
| - | * Extraction of the OD curve data (values)
| |
| - | * Replating Asaia on plates with and without kanamicin in order to recover the WT Asaia (only WT do not grow on kanamicin)
| |
| - | * Feeding and infection of Drosophila (WT and immunodeficient redish mutants) with bacteria Ecc15 and Asaia in Prof. Lemaitre Lab
| |
| - | * Fluorescence microscopy -> presence of fluorescence in the body/gut as expected
| |
| - | * Transformation of E-Coli with the ligation products and plating the transformed bacteria
| |
| | | | |
| | | | |
| - | =19-07-2010=
| |
| - | * Results of the transformation checked: successful!!
| |
| - | * Amplification of colonies (goal to do a miniprep of the plasmids --ori-- and --ori-resistance--)
| |
| - | * Results of plating of asaia culture (where we hope to recover the WT) checked: Asaia cultures grew on Kan plates also! Checked colonies for fluorescance under the microscope: no fluorescance --> Very unexpected! Don't know reason yet.
| |
| - | * Re-grow the colonies. Multiple cycles: hope that we get WT.
| |
| - | * Preparation of Asaia cultures for Lemaitre experiments/WT/Competence...
| |
| - | * Preparation of SOC medium and GLY medium
| |
| - | * Autoclaving of media and flasks
| |
| | | | |
| | | | |
| - | =20-07-2010=
| |
| - | * Plating Asaia to get WT (once again)
| |
| - | * Making HEPES Buffer for the Competence of Asaia
| |
| - | * Diluted the culture to get a 25ml culture(1:20) according to Asaia competence protocol
| |
| - | * OD curve plotted, Doubling time calculated
| |
| - | * No single colonies from the re-growing trial --> replating properly on separate plates
| |
| - | * Preparation of LB medium mixed with Kan/Amp/Cm and GLY medium mixed with <kan
| |
| - | * Miniprep of Origin + Resistance
| |
| - | * Miniprep of Origin alone.
| |
| - | * Miniprep of base vector BBa_151020
| |
| - | * Concentration measurments of miniprep products
| |
| - | * Glycerol stock of BB plasmid and Base Vector made
| |
| | | | |
| | | | |
| - | =21-07-2010=
| |
| - | * Measure OD of overnight culture of Asaia for competence --> value was too high
| |
| - | * Diluted culture to 0.3 OD, left in 30 degree incubator for 2 hours, OD of 0.6 measured --> start with competence
| |
| - | * Asaia competence
| |
| - | * Digestion of BB's produced and of the Base vector
| |
| - | * Agar plates with Kan/Tet/Cm/Amp made
| |
| - | * Prepared Gel with the products of the digestion to check if the fragments are as expected
| |
| - | * Prepare Tet stock (from powder)
| |
| | | | |
| | | | |
| - | =22-07-2010=
| |
| - | * Gel with products of digestion run --> only one of the base vector samples and a fragment with the origin and a Kan resistance worked. The others probably did not wok since the concentration of DNA was too low.
| |
| - | * Colonies picked and suspension cultures started. For making a miniprep tomorrow morning.
| |
| - | * Gel cut, Gel purification made
| |
| - | * New idea: PCR run on the product of the miniprep.
| |
| - | * Ligation of Base Vector with Origin+Kan
| |
| - | * Asaia preculture for OD growth (different pH) measurements
| |
| - | * Gly medium with pH 2,3,4,5,6,7.
| |
| - | * Research
| |
| - | * Replating of Asaia (Replating III) to get the WT (one day!!)
| |
| | | | |
| | | | |
| - | =23-07-2010=
| |
| - | * OD measurement growth curve in function of pH
| |
| - | * new DNA miniprep to extract Ori+AB_Res (the last one failed...)
| |
| - | * Lyse-n-Go protocol to do the ligase (and to check the insertion)
| |
| - | * transformation of the only digestion from yesterday that didn't failed (Kan)
| |
| - | * meeting with Onya for mosquito experiments
| |
| - | * preparation of 4L of Gly for LeMaitre experiments
| |
| | | | |
| | | | |
| - | =26-07-2010=
| |
| - | * Lemaître experiment: 400 ml cultures of GFP Asaia, Ecc15 and P.Entomophila
| |
| - | * Lemaître experiment: separating male and female drosophila
| |
| - | * Transformation of the Base Vector with -Kan-ori- insert into E.Coli DB3.1
| |
| - | * PCR of LovTab as a positive control to check if the PCR works (with own and Henrike's dNTP's) --> Run gel
| |
| - | * Test-digestion of some of the Mini-preps (Ori alone, Ori-Amp) --> Run gel
| |
| - | * Recovered the wildtype Asaia worked!!
| |
| - | * WT Asaia strains arrived. Culture started
| |
| | | | |
| | | | |
| - | =27-07-2010=
| |
| - | * Ligation C3/A3 + Ori + resistance (Kan, Amp, Tet, Cm)
| |
| - | * transformation of the ligation products into E.Coli DH5a
| |
| - | * Plating of transformed cells with selection of the vector and the insert
| |
| - | * Colony PCR of E.Coli DB3.1 containing BaseVector + Ori + Kan --> running gel (twice, the first time failed)
| |
| - | * Preculture of (maybe!!) WT Asaia for competence protocol
| |
| - | * Sorting of male/female flies
| |
| - | * Culture of 400 ml of different species of bacteria.
| |
| | | | |
| | | | |
| - | =28-07-2010=
| |
| - | * Analysis of plating of transformed cells
| |
| - | * Gel for check the Colony PCR
| |
| - | * 250 ml Gly medium
| |
| - | * OD measurement of preculture for competent Asaia --> too high. Start a new one!!!
| |
| - | * Competent Asaia and stocking @-80°C
| |
| - | * Plating of transformed E.Coli for double check
| |
| - | * preparation of the pellets for infection with 3 different species of bacteria + OD measurements
| |
| - | * preparation of tubes with 15 female flies
| |
| - | * begin of the survival experiment: infection of flies --> check of the flies after 2h
| |
| | | | |
| | | | |
| - | =29-07-2010=
| |
| - | * Results from plating of transformed E.Coli for double check and miniprep from some of them
| |
| - | * Digestion with different restriction enzymes to check if we get the right length
| |
| - | * Counting of dead flies one day after the infection
| |
| - | * Observation of fluorescence of infected flies under the microscope
| |
| | | | |
| | | | |
| - | =30-07-2010=
| |
| - | * Running a gel of the digested products of miniprep (29.07) --> bad results
| |
| - | * Miniprep for the last 17 samples of replating
| |
| - | * Digestion of 5 samples with 3 restriction enzymes to check if we get the fragments we expect
| |
| - | * Running a gel for the digestion products
| |
| - | * Counting of dead flies two days after the infection
| |
| - | * Observation of fluorescence of infected flies under the microscope
| |
| | | | |
| | | | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | |
| | + | = How to use the Notebook = |
| | + | |
| | + | This is a NOTEbook and not a LABbook. Here you can describe what you have done during your exciting day. If you want to have more details you can look in the IRL Labbook. Use ctrl+F to find something ;) |
| | + | |
| | + | == template of the day == |
| | + | <html> <table cellspacing="15" > <tr> <td> </html> |
| | + | For each new day in the lab you must create a new date in the notebook. To do so, copy-paste the following template: |
| | + | |
| | + | <pre> |
| | = 02-08-2010 = | | = 02-08-2010 = |
| | {| cellspacing="15" | | {| cellspacing="15" |
| | | style="width: 33.33%" valign="top" | | | | style="width: 33.33%" valign="top" | |
| | == Other == | | == Other == |
| - | We make some plate with GLY medium and Agar.
| + | It rain a lot today |
| | | | |
| | | style="width: 33.33%" valign="top" | | | | style="width: 33.33%" valign="top" | |
| | == Asaia == | | == Asaia == |
| - | Today we transform competent Asaia with a GFP + kanamycin (resistance) following this [https://static.igem.org/mediawiki/2010/a/ac/EPFL_prot_Electroporation_asaia.pdf protocols]. We plate it and we'll waiting 2 days to see if it works. | + | Today I speak with an asaia and she answer to me ^^ |
| - | we do digestion to create our first biobrick. We will make the ligation and the transformation in E-Coli tomorrow.
| + | |
| | | | |
| | | style="width: 33.33%" valign="top" | | | | style="width: 33.33%" valign="top" | |
| | == Drosophile == | | == Drosophile == |
| - | we count the drosophile and take photos with the fluorecense microscope. In some tubes the filter has detached so the expermiment for those tube are finished.
| + | |
| | |} | | |} |
| | + | </pre> |
| | | | |
| - | = 03-08-2010 =
| + | <html> </td><td> </html> |
| - | {| cellspacing="15"
| + | |
| - | | style="width: 33.3%" valign="top" |
| + | |
| - | == Immunotoxin ==
| + | |
| - | We have designed primers that overlap, generating a promoter and ribosome binding site. We want to put this in a vector which would be our basis to add other genes, for example our immunotoxin.
| + | |
| - | We designed a strong promoter+RBS and a weak one, to verifys that the strong one does not generate too much immunotoxin and might kill the bacteria...
| + | |
| | | | |
| | + | <u>Here are some useful explanations : </u> |
| | | | |
| - | | style="width: 33.3%" valign="top" |
| + | 1. The date format is important! |
| | | | |
| - | == Wiki == | + | 2. Just after the date, put the following two lines : |
| - | We did a lot of work on the wiki but we haven't downloaded it yet. Tomorrow you will see!
| + | <pre> |
| | + | {| cellspacing="15" |
| | + | | style="width: 33.33%" valign="top" | |
| | + | </pre> |
| | + | 3. Beetween each subtitle, put this line : |
| | + | <pre> |
| | + | | style="width: 33.33%" valign="top" | |
| | + | </pre> |
| | + | 4. To end the day just put : |
| | + | <pre> |
| | + | |} |
| | + | </pre> |
| | + | 5. Of course the text "width:33.33%" must be adapted on how many subtitle you have (for instance 4 subtitles = 25%) |
| | | | |
| | + | <html></td></tr></table></html> |
| | | | |
| - | | style="width: 33.3%" valign="top" |
| |
| | | | |
| - | ==Asaia==
| |
| - | We keep counting the drosofilia and taking pictures with the GFP filter.
| |
| | | | |
| - | |}
| |
| | | | |
| - | = 04-08-2010 =
| + | {{EPFL_2010_bottom_wrap}} |
| - | {| cellspacing="15" | + | |
| - | | style="width: 50%" valign="top" |
| + | |
| - | == Asaia ==
| + | |
| - | The asaia transformation we let in the incubator since 02-08 have now colonies! Today we must see can see the GFP!
| + | |
| - | | + | |
| - | | style="width: 50%" valign="top" |
| + | |
| - | == Wiki ==
| + | |
| - | | + | |
| - | | + | |
| - | |}
| + | |
| | | | |
| | </div> | | </div> |
"
