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Team:Cambridge/Bioluminescence/Photinus pyralis
From 2010.igem.org
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| - | {{:Team:Cambridge/Templates/headerbar|colour=#96d446|title= | + | {{:Team:Cambridge/Templates/headerbar|colour=#96d446|linkcolour=#557A25|title=Project Firefly: Photinus pyralis}} |
| - | + | <html><div style="float:right; padding-left:50px;"> | |
| + | <img src="https://static.igem.org/mediawiki/2010/thumb/f/f2/Firefly.jpg/300px-Firefly.jpg"> | ||
| + | <br /> | ||
| + | <i>Photinus pyralis (<a href="http://www.flickr.com/photos/artfarmer/198487523/">source</a>)</i> | ||
| + | </div> | ||
| + | </html> | ||
| - | + | The luciferase of the North American firefly, ''P. pyralis'', is a tried and tested mechanism for creating bioluminescence. We were aware that a luciferase from this organism was already present in the registry ([http://partsregistry.org/Part:BBa_I712019 BBa_I712019]). We wanted to improve on this by three techniques: | |
| + | * [https://2010.igem.org/Team:Cambridge/Codons Codon optimisation] for expression in E. coli to increase the rate of translation | ||
| + | * Using a mutant with increased substrate affinity | ||
| + | * Parallel use of the Photinus pyralis [https://2010.igem.org/Team:Cambridge/Bioluminescence/Luciferin_Regeneration luciferin regenerating enzyme] to both relieve inhibition by oxyluciferin and increase availability of luciferin. | ||
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{{:Team:Cambridge/Templates/footer}} | {{:Team:Cambridge/Templates/footer}} | ||
Latest revision as of 09:46, 18 October 2010
Project Firefly: Photinus pyralis
The luciferase of the North American firefly, P. pyralis, is a tried and tested mechanism for creating bioluminescence. We were aware that a luciferase from this organism was already present in the registry ([http://partsregistry.org/Part:BBa_I712019 BBa_I712019]). We wanted to improve on this by three techniques:
- Codon optimisation for expression in E. coli to increase the rate of translation
- Using a mutant with increased substrate affinity
- Parallel use of the Photinus pyralis luciferin regenerating enzyme to both relieve inhibition by oxyluciferin and increase availability of luciferin.
