Team:UIUC-Illinois/Safety

From 2010.igem.org

(Difference between revisions)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
{{Template:UIUC_Illinois_Bioware_Background}}
{{Template:UIUC_Illinois_Bioware_Background}}
-
<!--- The Mission, Experiments --->
+
{{Template:UIUC_Illinois_Bioware}}
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
==Safety==
-
!align="center"|[[Team:UIUC-Illinois|Home]]
+
-
!align="center"|[[Team:UIUC-Illinois/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=UIUC-Illinois Official Team Profile]
+
-
!align="center"|[[Team:UIUC-Illinois/Project|Project]]
+
-
!align="center"|[[Team:UIUC-Illinois/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:UIUC-Illinois/Modeling|Modeling]]
+
-
!align="center"|[[Team:UIUC-Illinois/Notebook|Notebook]]
+
-
!align="center"|[[Team:UIUC-Illinois/Safety|Safety]]
+
-
|}
+
-
<!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** -->
+
Safety was a huge factor when designing our project, and our team has spent a lot of time discussing potential safety hazards associated with our project. Following are the conclusions that we came to.
-
<html>
+
'''Researcher safety issues:'''
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
-
This is a template page. READ THESE INSTRUCTIONS.
+
-
</div>
+
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
-
</div>
+
-
</html>
+
-
 
+
-
<!-- *** End of the alert box *** -->
+
-
 
+
-
 
+
-
 
+
-
{|align="justify"
+
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
-
|
+
-
|-
+
-
|
+
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
-
|
+
-
|-
+
-
|
+
-
|
+
-
|}
+
-
 
+
-
 
+
-
[[Image:UIUC-Illinois_team.png|600px|left]] [[Image:UIUC-Illinois_logo.png|200px|right|frame]]
+
-
<br>
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
 
+
-
==Safety==
+
-
 
+
-
'''Potential safety issues:'''
+
<ul>
<ul>
   <li>The team worked with high levels of arsenic and gold in solution.  The risk of harmful exposure to these heavy metals was mitigated by adhering to stringent lab safety techniques.
   <li>The team worked with high levels of arsenic and gold in solution.  The risk of harmful exposure to these heavy metals was mitigated by adhering to stringent lab safety techniques.
Line 88: Line 12:
</ul>
</ul>
-
'''Aspects of project that mitigate safety issues:'''
+
'''Environmental and public safety issues:'''
<ul>
<ul>
-
   <li>The <i>E.coli</i> chassis used for our experimentation is not infectious.   
+
   <li>The <i>E.coli</i> chassis used for our experimentation was not infectious.   
   <li>Our biological system does not produce any toxic substances.   
   <li>Our biological system does not produce any toxic substances.   
-
   <li>Experimentation took place in a BL2 laboratory, while our work only required the use of a BL1 lab.  
+
   <li>Experimentation took place in a BL2 laboratory, while our work only required the use of BL1.  
</ul>   
</ul>   
-
Please use this page to answer the safety questions posed on the [[Safety | safety page]].
+
The University of Illinois' [http://www.drs.illinois.edu/bss/about.aspx?tbID=ab Biological Safety Section] of the Division of Research Safety is aware of our project and decided that working under the jurisdiction of Dr. Yong-Su Jin was sufficient for safety guidelines to be met.  In addition, all team members were required to pass both general and biological laboratory safety and protocol tests before working in lab.
 +
 
 +
 
 +
----

Latest revision as of 01:13, 27 October 2010

Click to go to the Illinois home page

Safety

Safety was a huge factor when designing our project, and our team has spent a lot of time discussing potential safety hazards associated with our project. Following are the conclusions that we came to.

Researcher safety issues:

  • The team worked with high levels of arsenic and gold in solution. The risk of harmful exposure to these heavy metals was mitigated by adhering to stringent lab safety techniques.
  • When performing agarose gel electrophoresis, ethidium bromide was used to stain our gels so that bands of nucleic acid could be viewed under ultraviolet light. Ethidium bromide is a known carcinogen, so proper precaution was observed when handling it. For example, no team member ever handled the compound without gloves, and it was never mixed with the agarose solution until after it had cooled to the point that no vapor was emitted from the flask. Also, our stock of ethidium bromide was stored away from other frequently used materials and wrapped in aluminum foil.

Environmental and public safety issues:

  • The E.coli chassis used for our experimentation was not infectious.
  • Our biological system does not produce any toxic substances.
  • Experimentation took place in a BL2 laboratory, while our work only required the use of BL1.


The University of Illinois' [http://www.drs.illinois.edu/bss/about.aspx?tbID=ab Biological Safety Section] of the Division of Research Safety is aware of our project and decided that working under the jurisdiction of Dr. Yong-Su Jin was sufficient for safety guidelines to be met. In addition, all team members were required to pass both general and biological laboratory safety and protocol tests before working in lab.