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Revision as of 19:17, 2 August 2010 (view source) Ebrinkman (Talk | contribs) ← Older edit		Latest revision as of 08:28, 26 August 2010 (view source) Hugo 87 (Talk | contribs) (→Lab work)
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		+	==Biobricks==
		+	By Hugo
		+
		+	I've got some colonies in the plates, however there's contamination with some yellow colonies :s
		+	Probably, we will have to prepare a new stock of competent cells.

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Latest revision as of 08:28, 26 August 2010

Contents 1 Cleaning 2 Lab work 2.1 Emulsifier 2.2 Biobricks

Cleaning

After all the media attention, we had a nice slow day. We finished some small experiments and reorganized our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.

We also booked a hotel in Boston!

Lab work

Emulsifier

Pieter worked on the emulsifying activity assay. Four samples were tested:

1. Mix, 0.02 mL of substrate, 0.1- 0.5 mL of sample.

#	Amount of SDS (%)	Hexalkane (mL)
1	0	0
2	0	0.02
3	0.01	0.02
4	1	0.02

2. Tris buffer (pH 8) was added to a total volume of 7.5 ml.

3. Shake 1 hour with 200 strokes/min at 30°C

4. Measure absorbance OD600

#	A600
1	0
2	0
3	0.4
4	0.2

Biobricks

By Hugo

I've got some colonies in the plates, however there's contamination with some yellow colonies :s Probably, we will have to prepare a new stock of competent cells.