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2010.igem.org/Team:Harvard/fences/notebook
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Our lab notebook on OpenWetWare can be found [http://openwetware.org/wiki/IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010 here]. | Our lab notebook on OpenWetWare can be found [http://openwetware.org/wiki/IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010 here]. | ||
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| + | ==June 14 2010== | ||
| + | <b>LacI Transformation</b> | ||
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| + | Performed bacterial transformation according to [[Silver:_Bacterial_Transformation]], however we used 1μL in step 3, and used entire tube of TOP10 competent cells instead of only 10-15μL. | ||
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| + | Transformed the following, each into its own tube of TOP10 E.Coli: | ||
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| + | 1) LacI with a rapid degredation tail attached, biobrick BBa_C0012, located on plate 1, well 2O, plasmid pSB1A2 | ||
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| + | 2) LacI wildtype, biobrick BBa_I732100, located on plate 2, well 10E, plasmid pSB1A3 | ||
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| + | Streaked 100μL each of 1 and 2 onto separate LB + Amp dishes, along with a control dish of 100μL untransformed TOP10 E.Coli cells. | ||
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| + | Plates incubated overnight, colonies observed in all plates except the control. | ||
Latest revision as of 16:29, 10 October 2010
Our lab notebook on OpenWetWare can be found [http://openwetware.org/wiki/IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010 here].
June 14 2010
LacI Transformation
Performed bacterial transformation according to Silver:_Bacterial_Transformation, however we used 1μL in step 3, and used entire tube of TOP10 competent cells instead of only 10-15μL.
Transformed the following, each into its own tube of TOP10 E.Coli:
1) LacI with a rapid degredation tail attached, biobrick BBa_C0012, located on plate 1, well 2O, plasmid pSB1A2
2) LacI wildtype, biobrick BBa_I732100, located on plate 2, well 10E, plasmid pSB1A3
Streaked 100μL each of 1 and 2 onto separate LB + Amp dishes, along with a control dish of 100μL untransformed TOP10 E.Coli cells.
Plates incubated overnight, colonies observed in all plates except the control.
