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Revision as of 21:04, 29 July 2010 (view source) AndreasConstantinou (Talk | contribs) (New page: {{Stockholm/Top2}} ==Andreas== ===CPP troubleshooting=== Analyzed sequencing results of our constructed CPPs (LMWP and Transportan 10). It seems like our target vector recircularizes ins...) ← Older edit		Latest revision as of 10:50, 26 October 2010 (view source) JohanNordholm (Talk | contribs) m
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-	==Andreas==	+	= Mimmi =
-	===CPP troubleshooting===	+	=== flourecent pEX ===
-	Analyzed sequencing results of our constructed CPPs (LMWP and Transportan 10). It seems like our target vector recircularizes instead of ligating with our CPP primers. Me and Johan are working on troubleshooting this, however it looks like we will soon have to synthesize our genes, since our primer ligations just don't seem to work.	+
		+	==== over expression ====
		+
		+	*ON culture
		+	**12ml LB
		+	**24µl Amp
		+	**small pipette tip of BL21.pEX.BBa_J18930 in glycerol
		+
		+	{{Stockholm/Footer}}

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Latest revision as of 10:50, 26 October 2010

Mimmi

flourecent pEX

over expression

• ON culture
  • 12ml LB
  • 24µl Amp
  • small pipette tip of BL21.pEX.BBa_J18930 in glycerol