Team:Harvard/vectors/parts

From 2010.igem.org

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<h1>parts and primers</h1><br />
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<div id="abstract">
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<h2>Vectors</h2>
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<h1>parts and primers</h1>
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For more information on the vectors visit our "<a href="vectors.html">meet the vectors</a>" page<br /><br />
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<h2>vectors</h2>
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For more information on the vectors visit our "<a href="https://2010.igem.org/Team:Harvard/vectors/vectors">meet the vectors</a>" page<br /><br />
<table cellspacing="0">
<table cellspacing="0">
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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr>
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<tr><td><b>Name</b></td><td><b>Biobrick Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Resistance (Plants)</b></td><td><b>Sequence</b></td></tr>
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<tr><td>V1</td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V1</td><td><center>-</center></td><td>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb">link</a></td></tr>
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<tr><td>V2</td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V2</td><td><center>-</center></td><td>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fc/PORE_O2.gb">link</a></td></tr>
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<tr><td>V3</td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V3</td><td><center>-</center></td><td>pORE Expression Series 3 (E3)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/e/e7/PORE_E3.gb">link</a></td></tr>
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<tr><td>V4</td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V4</td><td><center>-</center></td><td>pORE Expression Series 4 (E4)</td><td>Agrobacterium vector with ENTCUP2 promoter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/b/b9/PORE_E4.gb">link</a></td></tr>
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<tr><td>V5</td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V5</td><td><center>-</center></td><td>pORE Reporter Series 1 (R1)</td><td>Agrobacterium vector with gusA reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fe/PORE_R1.gb">link</a></td></tr>
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<tr><td>V6</td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V6</td><td><center>-</center></td><td>pORE Reporter Series 3 (R3)</td><td>Agrobacterium vector with smgfp reporter</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/e/e8/PORE_R3.gb">link</a></td></tr>
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<tr><td>V7</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V7</td><td>BBa_K382000</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/3b/V7.gb">link</a></td></tr>
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<tr><td>V8</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V8</td><td>BBa_K382001</td><td><center>-</center></td><td>Agrobacterium vector open series with biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/0/0f/V8.gb">link</a></td></tr>
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<tr><td>V9</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td></td></tr>
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<tr><td>V9</td><td>BBa_K382002</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/d/d2/V9.gb">link</a></td></tr>
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<tr><td>V10</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V10</td><td>BBa_K382003</td><td><center>-</center></td><td>Agrobacterium vector with ENTCUP2 promoter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/3/33/V10.gb">link</a></td></tr>
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<tr><td>V11</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V11</td><td>BBa_K382004</td><td><center>-</center></td><td>Agrobacterium vector with gusA reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/5/56/V11.gb">link</a></td></tr>
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<tr><td>V12</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td></td></tr>
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<tr><td>V12</td><td>BBa_K382005</td><td><center>-</center></td><td>Agrobacterium vector with smgfp reporter and biobrick cloning site</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/a/a8/V12.gb">link</a></td></tr>
</table>
</table>
<br /><br />
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<h2>Primers</h2>
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<h2>primers</h2>
<table cellspacing="0">
<table cellspacing="0">
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
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<tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr>
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<tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP3">link</a></td></tr>
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<tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td></td></tr>
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<tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP4">link</a></td></tr>
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<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP5">link</a></td></tr>
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<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP6">link</a></td></tr>
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<tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP7">link</a></td></tr>
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<tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP8">link</a></td></tr>
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<tr><td>VP9</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>VP9</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP9">link</a></td></tr>
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<tr><td>VP11</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>VP11</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td><a href="https://2010.igem.org/Team:Harvard/VP11">link</a></td></tr>
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<tr><td>VP12</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP12</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP12">link</a></td></tr>
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<tr><td>VP13</td><td>2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP13</td><td>2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP13">link</a></td></tr>
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<tr><td>VP14</td><td>3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP14</td><td>3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP14">link</a></td></tr>
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<tr><td>VP15</td><td>4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP15</td><td>4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP15">link</a></td></tr>
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<tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP16">link</a></td></tr>
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<tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP17">link</a></td></tr>
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<tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP18">link</a></td></tr>
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<tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP19">link</a></td></tr>
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<tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP20">link</a></td></tr>
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<tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP21">link</a></td></tr>
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<tr><td>VP22</td><td>6 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP22</td><td>6 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP22">link</a></td></tr>
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<tr><td>VP23</td><td>7 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP23</td><td>7 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP23">link</a></td></tr>
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<tr><td>VP24</td><td>8 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP24</td><td>8 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP24">link</a></td></tr>
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<tr><td>VP25</td><td>9 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>VP25</td><td>9 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td><a href="https://2010.igem.org/Team:Harvard/VP25">link</a></td></tr>
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<tr><td>VP26</td><td>1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP26</td><td>1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP26">link</a></td></tr>
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<tr><td>VP27</td><td>2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP27</td><td>2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP27">link</a></td></tr>
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<tr><td>VP28</td><td>3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP28</td><td>3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP28">link</a></td></tr>
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<tr><td>VP29</td><td>4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP29</td><td>4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP29">link</a></td></tr>
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<tr><td>VP30</td><td>5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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<tr><td>VP30</td><td>5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td><a href="https://2010.igem.org/Team:Harvard/VP30">link</a></td></tr>
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<tr><td>VP33</td><td>1 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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<tr><td>VP31</td><td>1 of 2 for sequencing V7 and V8 biobrick cloning site</td><td><a href="https://2010.igem.org/Team:Harvard/VP31">link</a></td></tr>
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<tr><td>VP34</td><td>2 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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<tr><td>VP34</td><td>2 of 2 for sequencing V7 and V8 biobrick cloning site</td><td><a href="https://2010.igem.org/Team:Harvard/VP34">link</a></td></tr>
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Latest revision as of 23:19, 26 October 2010



parts and primers

vectors

For more information on the vectors visit our "meet the vectors" page

NameBiobrick NameOriginal NameDescriptionResistanceResistance (Plants)Sequence
V1
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pORE Open Series 1 (O1)Agrobacterium vector open seriesKanamycinpatlink
V2
-
pORE Open Series 2 (O2)Agrobacterium vector open seriesKanamycinnptIIlink
V3
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pORE Expression Series 3 (E3)Agrobacterium vector with ENTCUP2 promoterKanamycinpatlink
V4
-
pORE Expression Series 4 (E4)Agrobacterium vector with ENTCUP2 promoterKanamycinnptIIlink
V5
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pORE Reporter Series 1 (R1)Agrobacterium vector with gusA reporterKanamycinnptIIlink
V6
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pORE Reporter Series 3 (R3)Agrobacterium vector with smgfp reporterKanamycinnptIIlink
V7BBa_K382000
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Agrobacterium vector open series with biobrick cloning siteKanamycinpatlink
V8BBa_K382001
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Agrobacterium vector open series with biobrick cloning siteKanamycinnptIIlink
V9BBa_K382002
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Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinpatlink
V10BBa_K382003
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Agrobacterium vector with ENTCUP2 promoter and biobrick cloning siteKanamycinnptIIlink
V11BBa_K382004
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Agrobacterium vector with gusA reporter and biobrick cloning siteKanamycinnptIIlink
V12BBa_K382005
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Agrobacterium vector with smgfp reporter and biobrick cloning siteKanamycinnptIIlink


primers

NameDescriptionSequence
VP3HindIII-pENTCUP2 forward primer for Expression Series insertlink
VP4pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insertlink
VP5HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insertlink
VP6gusA-NheI reverse primer for Reporter Series 1 insertlink
VP7HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insertlink
VP8smGFP-NheI reverse primer for Reporter Series 1 insertlink
VP9Biobrick multiple cloning site for annealing with VP10 to create Open Series Insertlink
VP11Biobrick multiple cloning site for annealing with VP10 to create Open Series Insertlink
VP121 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP132 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP143 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP154 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP165 of 5 for sequencing V12 biobrick cloning site and smgfp reporterlink
VP171 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP182 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP193 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP204 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP215 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP226 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP237 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP248 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP259 of 9 for sequencing V11 biobrick cloning site and gusA reporterlink
VP261 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP272 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP283 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP294 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP305 of 5 for sequencing V9 and V10 biobrick cloning site and promoterlink
VP311 of 2 for sequencing V7 and V8 biobrick cloning sitelink
VP342 of 2 for sequencing V7 and V8 biobrick cloning sitelink

Retrieved from "http://2010.igem.org/Team:Harvard/vectors/parts"