Team:Cambridge/References/ProjectBioluminescence/LightLevel

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Latest revision as of 12:18, 7 October 2010

Bioluminescence: Light Output

Increasing Light Emission

[http://www.ncbi.nlm.nih.gov/pubmed/12234677 Paper on isolation of G-LRE and H-LRE, which lists previous attempts to improve light production]
[http://www.ncbi.nlm.nih.gov/pubmed/10036167 Intensified light emission in presence of low ATP concentration by PPDK]
[http://www.ncbi.nlm.nih.gov/pubmed/1332531 Enhanced luciferase activity through cytodine nucleotides]
[http://www.ncbi.nlm.nih.gov/pubmed/12044905 Gene chimerisation to improve practical usefulness of firefly luciferase]
[http://www.ncbi.nlm.nih.gov/pubmed/17540326 Genetically modified firefly luciferase - the EPIC luciferase]
[http://www.springerlink.com/content/76q433v347503k17/ Bioluminescence Spectra of Native and Mutant Firefly Luciferases as a Function of pH]

Relevant Physics

The [http://en.wikipedia.org/wiki/Candela Candela] measures how much light perceivable to the human eye is being emitted by a source of radiation, the Luminous Intensity (or luminous flux. It is related to [http://en.wikipedia.org/wiki/Radient_intensity Radiant intensity] (or radiant flux) (which is measured in watts per steradian) but has been weighted by a [http://en.wikipedia.org/wiki/Luminosity_function Luminosity function]. This alters the power output so that it is proportional to the sensitivity of the human eye: [http://en.wikipedia.or/wiki/Candela#Explanation Definition] of the candela with respect to Radiant intensity. Therefore, an object which is black may be emitting a lot of radiation (measured in watts per steradian) but since it is not perceivable to the human eye it is emitting 0cd of radiation.
The [http://en.wikipedia.org/wiki/Lumen_%28unit%29 lumen] is simply cd*sr. It is thus the analogue to the Watt

The [http://en.wikipedia.org/wiki/Lux Lux] measures how much light is incident on a suface, the Illuminance. It is related to irradiance (measured in Watts per m^2) but has been weighted again by the luminosity function. 1lux= 1lm/m^2 = 1cd*1sr/m^2
The luminance of an object is how much light is emitted from it. This is also measured in Lux, but it is different contextually.

A table of ambient illuminances. For our glowing bacteria to be seen from a given distance, they must be at least 1/20th of this value.

Illuminance	Example
10⁻⁵ lux	Light from Sirius, the brightest star in the night sky
10⁻⁴ lux	Total starlight, overcast sky
0.002 lux	Moonless clear night sky with airglow
0.01 lux	Quarter moon
0.27 lux	Full moon on a clear night
1 lux	Full moon overhead at tropical latitude
3.4 lux	Dark limit of civil twilight under a clear sky
50 lux	Family living room
80 lux	Hallway/toilet
100 lux	Very dark overcast day
320–500 lux	Office lighting
400 lux	Sunrise or sunset on a clear day.
1,000 lux	Overcast day
10,000–25,000 lux	Full daylight (not direct sun)
32,000–130,000 lux	Direct sunlight

A brief [http://en.wikipedia.org/wiki/Candela#SI_photometric_light_units summary] of the different luminous measures

A [http://cvision.ucsd.edu/lumindex.htm site] containing luminosity functions

To summarise, the luminosity function allows one to convert between radiant energy and luminous (i.e., visible) energy.

Difficulties

Inclusion Bodies

[http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TCW-4D5X6SM-4N-1&_cdi=5181&_user=1495569&_pii=0167779988900650&_orig=search&_coverDate=05%2F31%2F1988&_sk=999939994&view=c&wchp=dGLbVlb-zSkWb&md5=7b765deb78f66afdf695ef56af3219d2&ie=/sdarticle.pdf Inclusion Bodies in E. Coli]

Human Vision

Spectrum interpolation

[http://iopscience.iop.org/0957-0233/17/5/S17/pdf/0957-0233_17_5_S17.pdf Radial-basis function network for the approximation of FBG sensor spectra with distorted peaks]
[http://www.cs.clemson.edu/~dhouse/courses/405/notes/splines.pdf Splines]

Source Code

Outputting lux

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-{{:Team:Cambridge/Templates/header}}
+{{:Team:Cambridge/Templates/headerMinimalprototype}}
-{{:Team:Cambridge/LumNavTemplate}}
+{{:Team:Cambridge/Templates/RefBar}}
+{{:Team:Cambridge/Templates/headerbar|colour=#96d446|title=Bioluminescence: Light Output}}
-=Light Output=
 ==Increasing Light Emission==
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 *The [http://en.wikipedia.org/wiki/Lumen_%28unit%29 lumen] is simply cd*sr. It is thus the analogue to the Watt
-*The [http://en.wikipedia.org/wiki/Lux Lux] measures how much light is incident on a suface, the Illuminance. It is related to irradiance (measured in Watts per m^2) but has been weighted again by the luminosity function. 1lux=1cd/m^2
+*The [http://en.wikipedia.org/wiki/Lux Lux] measures how much light is incident on a suface, the Illuminance. It is related to irradiance (measured in Watts per m^2) but has been weighted again by the luminosity function. 1lux= 1lm/m^2 = 1cd*1sr/m^2
 *The luminance of an object is how much light is emitted from it. This is also measured in Lux, but it is different contextually.
@@ Line 62: / Line 61: @@
 ==Difficulties==
-===Inclusion Bodies===
+==Inclusion Bodies==
 * [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TCW-4D5X6SM-4N-1&_cdi=5181&_user=1495569&_pii=0167779988900650&_orig=search&_coverDate=05%2F31%2F1988&_sk=999939994&view=c&wchp=dGLbVlb-zSkWb&md5=7b765deb78f66afdf695ef56af3219d2&ie=/sdarticle.pdf Inclusion Bodies in E. Coli]
@@ Line 73: / Line 72: @@
 * [http://www.cs.clemson.edu/~dhouse/courses/405/notes/splines.pdf Splines]
+==Source Code==
-{{:Team:Cambridge/Templates/footer}}
+*[[Team:Cambridge/ProjectBioluminescence/LightLevel/SourceCode|Outputting lux]]
+<html>
+</div>
+</html>
+{{:Team:Cambridge/Templates/footerMinimal}}