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Team:EPF Lausanne/Calendar/0727
From 2010.igem.org
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* Colony PCR of E.Coli DB3.1 containing BaseVector + Ori + Kan --> running gel (twice, the first time failed) | * Colony PCR of E.Coli DB3.1 containing BaseVector + Ori + Kan --> running gel (twice, the first time failed) | ||
* Preculture of (maybe!!) WT Asaia for competence protocol | * Preculture of (maybe!!) WT Asaia for competence protocol | ||
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| + | * Sorting of male/female flies | ||
| + | * Culture of 400 ml of different species of bacteria. | ||
</div> | </div> | ||
Latest revision as of 15:42, 28 July 2010
27 July 2010
- Ligation C3/A3 + Ori + resistance (Kan, Amp, Tet, Cm)
- transformation of the ligation products into E.Coli DH5a
- Plating of transformed cells with selection of the vector and the insert
- Colony PCR of E.Coli DB3.1 containing BaseVector + Ori + Kan --> running gel (twice, the first time failed)
- Preculture of (maybe!!) WT Asaia for competence protocol
- Sorting of male/female flies
- Culture of 400 ml of different species of bacteria.
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