"
Team:Cambridge/Quiescence/Sequences
From 2010.igem.org
Peteremmrich (Talk | contribs) |
Peteremmrich (Talk | contribs) (→Biobricked construct for assembly from oligos) |
||
| (12 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | {{:Team:Cambridge/Templates/ | + | {{:Team:Cambridge/Templates/headerMinimalprototype}} |
| + | {{:Team:Cambridge/Templates/headerbar|colour=#fb5c2b|title=Quiescence: Sequences}} | ||
| + | |||
| + | |||
Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95: | Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95: | ||
| Line 8: | Line 11: | ||
This sequence does not include an aptamer. | This sequence does not include an aptamer. | ||
| - | {{:Team:Cambridge/Templates/ | + | |
| + | ==Including aptamer== | ||
| + | |||
| + | AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA | ||
| + | uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa | ||
| + | ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU | ||
| + | UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG | ||
| + | GUGCCUACAAU | ||
| + | |||
| + | ==Rcd sequences== | ||
| + | Rcd 70 | ||
| + | |||
| + | AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | ||
| + | |||
| + | Rcd 95 | ||
| + | |||
| + | AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | ||
| + | AACGCGUUUUAGCGGUGCCUACAAU | ||
| + | |||
| + | ==Constructs== | ||
| + | Rcd70_aptazyme_theo+_30_3 | ||
| + | gAGCGCcugCACGGCAGCgcuCAGGUAAAAccGGCAACuugaCAgguccAAggcaGCCuuuAUaaugcCU | ||
| + | CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA|GGUU | ||
| + | GAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | ||
| + | |||
| + | Rcd70_aptazyme_theo+_28_9 | ||
| + | CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc|CGACCC | ||
| + | UGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGUUGAGGCGC | ||
| + | GAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | ||
| + | |||
| + | Rcd70_aptazyme_theo+_30_8 | ||
| + | CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc|CGACCCU | ||
| + | GAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU | ||
| + | UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG | ||
| + | |||
| + | ==Biobricked construct for assembly from oligos== | ||
| + | GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAAATGGCAACAAACCACCCGA | ||
| + | AAAACTGCCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAGCCAAGCG | ||
| + | AAACGGTGAAAGCCGTAGGTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTG | ||
| + | TCTGCTGCCGTGATCGCGCTGTACTAGTAGCGGCCGCTGCAG | ||
| + | |||
| + | ===PCR Oligo Maker output=== | ||
| + | GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAA | ||
| + | GCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCG | ||
| + | CCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAG | ||
| + | GCGATCGCGCCTCAACCTACGGCTTTCACCGTTTCGCTTGGCTGCCAAGGGCCTTTCG | ||
| + | GTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC | ||
| + | CTGCAGCGGCCGCTACTAGTACAGCGCGATCACGGCAGCAGACAGGTAAAAATGG | ||
| + | |||
| + | <html> | ||
| + | </div> | ||
| + | </html> | ||
| + | {{:Team:Cambridge/Templates/footerMinimal}} | ||
Latest revision as of 11:09, 13 August 2010
Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95:
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUcuuggagcugaugagccguucgcggcgaaaacu aAGCUUAUUGCUugguuacuccaagAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCA UUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCGGUGCCUACAAU
This sequence does not include an aptamer.
Including aptamer
AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG GUGCCUACAAU
Rcd sequences
Rcd 70
AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Rcd 95
AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG AACGCGUUUUAGCGGUGCCUACAAU
Constructs
Rcd70_aptazyme_theo+_30_3 gAGCGCcugCACGGCAGCgcuCAGGUAAAAccGGCAACuugaCAgguccAAggcaGCCuuuAUaaugcCU CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA|GGUU GAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Rcd70_aptazyme_theo+_28_9 CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc|CGACCC UGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGUUGAGGCGC GAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Rcd70_aptazyme_theo+_30_8 CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc|CGACCCU GAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
Biobricked construct for assembly from oligos
GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAAATGGCAACAAACCACCCGA AAAACTGCCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAGCCAAGCG AAACGGTGAAAGCCGTAGGTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTG TCTGCTGCCGTGATCGCGCTGTACTAGTAGCGGCCGCTGCAG
PCR Oligo Maker output
GAATTCGCGGCCGCTTCTAGAGCAGCGCGATCACGGCAGCAGACAGGTAAAA GCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCG CCGCGATCGCGCCTCGACCCTGATGAGTTTGAATACCAGCCGAAAGGCCCTTGGCAG GCGATCGCGCCTCAACCTACGGCTTTCACCGTTTCGCTTGGCTGCCAAGGGCCTTTCG GTTGAGGCGCGATCGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC CTGCAGCGGCCGCTACTAGTACAGCGCGATCACGGCAGCAGACAGGTAAAAATGG
