Team:Stockholm/26 July 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Mimmi == === LMWP C and N === Plasmid prep and gycerol stock The cells were cultured to 0,6 OD, 1,6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid ''...) |
m |
||
(6 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
+ | {{Stockholm/Top2}} | ||
+ | |||
== Mimmi == | == Mimmi == | ||
=== LMWP C and N === | === LMWP C and N === | ||
+ | ::'''Plasmid prep and glycerol stock''' | ||
- | |||
- | |||
+ | The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid | ||
- | '''DNA concentrations''' | + | ::'''DNA concentrations''' |
- | {|border="1" cellspacing="0 | + | ::{|border="1" cellspacing="0" |
- | + | ! Sample | |
- | + | ! DNA (ng/μl) | |
|- | |- | ||
|LMWP N | |LMWP N | ||
Line 20: | Line 22: | ||
|} | |} | ||
- | === over-expression in pEX - remaking the comassie-gel == | + | |
- | + | ||
- | + | === over-expression in pEX === | |
- | + | ::'''- remaking the comassie-gel''' | |
- | + | ||
- | Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON | + | |
+ | {|border="1" cellspacing="0" | ||
+ | ! Mix | ||
+ | | | ||
+ | |- | ||
+ | | LB | ||
+ | | 4ml | ||
+ | |- | ||
+ | | glycose | ||
+ | | 40µl (1%) | ||
+ | |- | ||
+ | | Amp | ||
+ | | 8µl (0.5%) | ||
+ | |} | ||
+ | |||
+ | |||
+ | *Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON | ||
+ | |||
+ | ---- | ||
+ | |||
+ | ==Nina== | ||
+ | |||
+ | ===Sequencing CPP transportan 10 version C=== | ||
+ | |||
+ | I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing. | ||
+ | |||
+ | * 15 ul vector | ||
+ | |||
+ | * 1.5 ul 10uM VR2 primer | ||
+ | |||
+ | 9: ASB003N X99 | ||
+ | |||
+ | 40: ASB003N X98 | ||
+ | |||
+ | ---- | ||
+ | |||
+ | ===Inoculation of IgG protease=== | ||
+ | |||
+ | I placed the inoculation samples in the refrigerator for mini prep the following day when I know which sample is suitable for miniprep. | ||
+ | |||
+ | {{Stockholm/Footer}} |
Latest revision as of 10:55, 26 October 2010
Contents |
Mimmi
LMWP C and N
- Plasmid prep and glycerol stock
The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid
- DNA concentrations
Sample DNA (ng/μl) LMWP N 60 LMWP C 80
over-expression in pEX
- - remaking the comassie-gel
Mix | |
---|---|
LB | 4ml |
glycose | 40µl (1%) |
Amp | 8µl (0.5%) |
- Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON
Nina
Sequencing CPP transportan 10 version C
I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing.
- 15 ul vector
- 1.5 ul 10uM VR2 primer
9: ASB003N X99
40: ASB003N X98
Inoculation of IgG protease
I placed the inoculation samples in the refrigerator for mini prep the following day when I know which sample is suitable for miniprep.