Team:Cambridge/References/ProjectBioluminescence/Experiments

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{{:Team:Cambridge/Templates/headerbar|colour=#96d446|title=Bioluminescence: Experiments}}
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=Experiments=
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==Firefly==
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[[Image:LRE_cycle_annotated.png |650px]]
[[Image:LRE_cycle_annotated.png |650px]]
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1. Test this part of the pathway with the Luciferase from the registry  
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==1. Test luminescence with the Luciferase from the registry==
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* Transform WT E.coli with the luciferase from the registry.
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* Feed with D-luciferin, develop a method for measuring light output
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* Variables - concentration of inducer (if luciferase promoter inducible), concentration of D-luciferin
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* Controls - no luciferin, no inducer
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==2. Test use of CHBT as a substrate==
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2. Test use of CHBT as a substrate
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With sucessfully transformed cells from 1 compare emission when adding:
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* D-luciferin
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* CHBT
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* CHBT + D-Cysteine
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* CHBT + L-Cysteine
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* (CHBT + L-Luciferin? if necessary)
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a) D-cysteine
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==3. Luciferin Regenerating Enzyme==
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b) L-cysteine via L-luciferin
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Latest revision as of 12:18, 7 October 2010

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