Team:EPF Lausanne/Labbook protocols

From 2010.igem.org

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= Test =
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= Lab Protocols =
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On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.
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*[https://static.igem.org/mediawiki/2010/archive/f/f9/20100727133405%21Prot_adapt_pH.pdf Adapt pH]
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*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel]
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*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production]
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*[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave]
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*[https://static.igem.org/mediawiki/2010/6/6b/Prot_asaia_competence.pdf Competent Asaia]
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*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis]
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*[https://static.igem.org/mediawiki/2010/8/82/Prot_growing_asaia.pdf Growing Asaia ]
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*[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation]
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*[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
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* Miniprep --> use Miniprep Kit (Protocol included in kit)
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*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)]
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*[https://static.igem.org/mediawiki/2010/5/54/Protocol_pcr_roche.pdf PCR (using the Roche Expand High Fidelity PCR System dNTPack ]
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*[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR (using iProof BIORAD)]
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* Purification --> use Purification Kit (Protocol included in kit)
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*[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells]
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*[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging]
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*Using the plate reader (Solange or Stefano)
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*[https://static.igem.org/mediawiki/2010/a/a2/Prot_digestion.pdf Digestion]
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= Media Recipes =
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*[https://static.igem.org/mediawiki/2010/archive/e/ed/20100715125103!Prot_LB.pdf LB medium]
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*[https://static.igem.org/mediawiki/2010/5/52/Prot_LB_agar.pdf LB agar medium]
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*[https://static.igem.org/mediawiki/2010/1/16/Prot_gly.pdf Gly medium]
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*[https://static.igem.org/mediawiki/2010/0/0e/Prot_gly_agar.pdf Gly agar medium]
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*[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium]
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= Syntax =
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Convention if you want to upload a new protocol. When uploading a file it please rename it in the following way:
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<i> EPFL_prot_yourProcedureName.yourExtension</i>
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Latest revision as of 19:36, 8 August 2010


Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

Media Recipes


Syntax

Convention if you want to upload a new protocol. When uploading a file it please rename it in the following way: 

 EPFL_prot_yourProcedureName.yourExtension


Retrieved from "http://2010.igem.org/Team:EPF_Lausanne/Labbook_protocols"