Team:Cambridge/References/ProjectBioluminescence/LightLevel
From 2010.igem.org
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- | =Light Output | + | {{:Team:Cambridge/Templates/headerMinimalprototype}} |
+ | {{:Team:Cambridge/Templates/RefBar}} | ||
+ | {{:Team:Cambridge/Templates/headerbar|colour=#96d446|title=Bioluminescence: Light Output}} | ||
==Increasing Light Emission== | ==Increasing Light Emission== | ||
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*[http://www.ncbi.nlm.nih.gov/pubmed/12044905 Gene chimerisation to improve practical usefulness of firefly luciferase] | *[http://www.ncbi.nlm.nih.gov/pubmed/12044905 Gene chimerisation to improve practical usefulness of firefly luciferase] | ||
*[http://www.ncbi.nlm.nih.gov/pubmed/17540326 Genetically modified firefly luciferase - the EPIC luciferase] | *[http://www.ncbi.nlm.nih.gov/pubmed/17540326 Genetically modified firefly luciferase - the EPIC luciferase] | ||
+ | *[http://www.springerlink.com/content/76q433v347503k17/ Bioluminescence Spectra of Native and Mutant Firefly Luciferases as a Function of pH] | ||
==Relevant Physics== | ==Relevant Physics== | ||
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*The [http://en.wikipedia.org/wiki/Lumen_%28unit%29 lumen] is simply cd*sr. It is thus the analogue to the Watt | *The [http://en.wikipedia.org/wiki/Lumen_%28unit%29 lumen] is simply cd*sr. It is thus the analogue to the Watt | ||
- | *The [http://en.wikipedia.org/wiki/Lux Lux] measures how much light is incident on a suface, the Illuminance. It is related to irradiance (measured in Watts per m^2) but has been weighted again by the luminosity function. 1lux=1cd/m^2 | + | *The [http://en.wikipedia.org/wiki/Lux Lux] measures how much light is incident on a suface, the Illuminance. It is related to irradiance (measured in Watts per m^2) but has been weighted again by the luminosity function. 1lux= 1lm/m^2 = 1cd*1sr/m^2 |
- | *The luminance of an object is how much light is emitted from it. This is also measured in Lux, but it is different contextually. | + | *The luminance of an object is how much light is emitted from it. This is also measured in Lux, but it is different contextually. |
- | + | ||
+ | * A table of ambient illuminances. For our glowing bacteria to be seen from a given distance, they must be at least 1/20th of this value. | ||
{| class="wikitable" | {| class="wikitable" | ||
!Illuminance!!Example | !Illuminance!!Example | ||
|- | |- | ||
- | |10<sup>−5</sup> lux ||Light from | + | |10<sup>−5</sup> lux ||Light from Sirius, the brightest star in the night sky |
|- | |- | ||
- | |10<sup>−4</sup> lux||Total | + | |10<sup>−4</sup> lux||Total starlight, overcast sky |
|- | |- | ||
- | |0.002 lux||Moonless clear night sky with | + | |0.002 lux||Moonless clear night sky with airglow |
|- | |- | ||
|0.01 lux||Quarter moon | |0.01 lux||Quarter moon | ||
Line 29: | Line 33: | ||
|0.27 lux||Full moon on a clear night | |0.27 lux||Full moon on a clear night | ||
|- | |- | ||
- | |1 lux||Full moon overhead at tropical | + | |1 lux||Full moon overhead at tropical latitude |
|- | |- | ||
- | |3.4 lux|| Dark limit of civil | + | |3.4 lux|| Dark limit of civil twilight under a clear sky |
|- | |- | ||
|50 lux||Family living room | |50 lux||Family living room | ||
Line 41: | Line 45: | ||
|320–500 lux || Office lighting | |320–500 lux || Office lighting | ||
|- | |- | ||
- | |400 lux || | + | |400 lux ||Sunrise or sunset on a clear day. |
|- | |- | ||
|1,000 lux||Overcast day | |1,000 lux||Overcast day | ||
- | |||
- | |||
|- | |- | ||
- | |32,000–130,000 lux||Direct | + | |10,000–25,000 lux ||Full daylight (not direct sun) |
+ | |- | ||
+ | |32,000–130,000 lux||Direct sunlight | ||
|} | |} | ||
Line 54: | Line 58: | ||
*A [http://cvision.ucsd.edu/lumindex.htm site] containing luminosity functions | *A [http://cvision.ucsd.edu/lumindex.htm site] containing luminosity functions | ||
- | *To summarise, the luminosity function allows one to convert between | + | *To summarise, the luminosity function allows one to convert between radiant energy and luminous (i.e., visible) energy. |
+ | |||
+ | ==Difficulties== | ||
+ | ==Inclusion Bodies== | ||
+ | * [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TCW-4D5X6SM-4N-1&_cdi=5181&_user=1495569&_pii=0167779988900650&_orig=search&_coverDate=05%2F31%2F1988&_sk=999939994&view=c&wchp=dGLbVlb-zSkWb&md5=7b765deb78f66afdf695ef56af3219d2&ie=/sdarticle.pdf Inclusion Bodies in E. Coli] | ||
==Human Vision== | ==Human Vision== | ||
+ | |||
+ | |||
+ | ==Spectrum interpolation== | ||
+ | |||
+ | * [http://iopscience.iop.org/0957-0233/17/5/S17/pdf/0957-0233_17_5_S17.pdf Radial-basis function network for the approximation of FBG sensor spectra with distorted peaks] | ||
+ | * [http://www.cs.clemson.edu/~dhouse/courses/405/notes/splines.pdf Splines] | ||
+ | |||
+ | ==Source Code== | ||
+ | *[[Team:Cambridge/ProjectBioluminescence/LightLevel/SourceCode|Outputting lux]] | ||
+ | <html> | ||
+ | </div> | ||
+ | </html> | ||
+ | {{:Team:Cambridge/Templates/footerMinimal}} |
Latest revision as of 12:18, 7 October 2010
Link dumps: Bioluminescence |
Firefly luciferases|
Lucferin recovery|
Light output|
Experiments|
Modelling
Bioluminescence: Light Output
Increasing Light Emission
- [http://www.ncbi.nlm.nih.gov/pubmed/12234677 Paper on isolation of G-LRE and H-LRE, which lists previous attempts to improve light production]
- [http://www.ncbi.nlm.nih.gov/pubmed/10036167 Intensified light emission in presence of low ATP concentration by PPDK]
- [http://www.ncbi.nlm.nih.gov/pubmed/1332531 Enhanced luciferase activity through cytodine nucleotides]
- [http://www.ncbi.nlm.nih.gov/pubmed/12044905 Gene chimerisation to improve practical usefulness of firefly luciferase]
- [http://www.ncbi.nlm.nih.gov/pubmed/17540326 Genetically modified firefly luciferase - the EPIC luciferase]
- [http://www.springerlink.com/content/76q433v347503k17/ Bioluminescence Spectra of Native and Mutant Firefly Luciferases as a Function of pH]
Relevant Physics
- The [http://en.wikipedia.org/wiki/Candela Candela] measures how much light perceivable to the human eye is being emitted by a source of radiation, the Luminous Intensity (or luminous flux. It is related to [http://en.wikipedia.org/wiki/Radient_intensity Radiant intensity] (or radiant flux) (which is measured in watts per steradian) but has been weighted by a [http://en.wikipedia.org/wiki/Luminosity_function Luminosity function]. This alters the power output so that it is proportional to the sensitivity of the human eye: [http://en.wikipedia.or/wiki/Candela#Explanation Definition] of the candela with respect to Radiant intensity. Therefore, an object which is black may be emitting a lot of radiation (measured in watts per steradian) but since it is not perceivable to the human eye it is emitting 0cd of radiation.
- The [http://en.wikipedia.org/wiki/Lumen_%28unit%29 lumen] is simply cd*sr. It is thus the analogue to the Watt
- The [http://en.wikipedia.org/wiki/Lux Lux] measures how much light is incident on a suface, the Illuminance. It is related to irradiance (measured in Watts per m^2) but has been weighted again by the luminosity function. 1lux= 1lm/m^2 = 1cd*1sr/m^2
- The luminance of an object is how much light is emitted from it. This is also measured in Lux, but it is different contextually.
- A table of ambient illuminances. For our glowing bacteria to be seen from a given distance, they must be at least 1/20th of this value.
Illuminance | Example |
---|---|
10−5 lux | Light from Sirius, the brightest star in the night sky |
10−4 lux | Total starlight, overcast sky |
0.002 lux | Moonless clear night sky with airglow |
0.01 lux | Quarter moon |
0.27 lux | Full moon on a clear night |
1 lux | Full moon overhead at tropical latitude |
3.4 lux | Dark limit of civil twilight under a clear sky |
50 lux | Family living room |
80 lux | Hallway/toilet |
100 lux | Very dark overcast day |
320–500 lux | Office lighting |
400 lux | Sunrise or sunset on a clear day. |
1,000 lux | Overcast day |
10,000–25,000 lux | Full daylight (not direct sun) |
32,000–130,000 lux | Direct sunlight |
- A brief [http://en.wikipedia.org/wiki/Candela#SI_photometric_light_units summary] of the different luminous measures
- A [http://cvision.ucsd.edu/lumindex.htm site] containing luminosity functions
- To summarise, the luminosity function allows one to convert between radiant energy and luminous (i.e., visible) energy.
Difficulties
Inclusion Bodies
- [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TCW-4D5X6SM-4N-1&_cdi=5181&_user=1495569&_pii=0167779988900650&_orig=search&_coverDate=05%2F31%2F1988&_sk=999939994&view=c&wchp=dGLbVlb-zSkWb&md5=7b765deb78f66afdf695ef56af3219d2&ie=/sdarticle.pdf Inclusion Bodies in E. Coli]
Human Vision
Spectrum interpolation
- [http://iopscience.iop.org/0957-0233/17/5/S17/pdf/0957-0233_17_5_S17.pdf Radial-basis function network for the approximation of FBG sensor spectra with distorted peaks]
- [http://www.cs.clemson.edu/~dhouse/courses/405/notes/splines.pdf Splines]
Source Code