NanoDrop Spectrophotometer

From 2010.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 1: Line 1:
 +
[[Image:Newcastle_nanodrop_2.jpeg|thumb|300px]]
 +
[[Image:Newcastle_nanodrop_1.jpeg|thumb|300px]]
 +
[[Image:Newcastle_nanodrop_3.jpeg|thumb|300px]]
Nanodrop can be used to measure the DNA, RNA and protein
Nanodrop can be used to measure the DNA, RNA and protein
Line 6: Line 9:
== Method: ==
== Method: ==
-
 
-
#Log onto computer, open program ND 1000
 
-
#Wipe pedestal and top. Apply 3 µl of water to nib of pedestal and initiliase program
 
-
#Wipe, apply elution buffer (EB) or appropriate blank control (3 µl) to nib, blank the system, set to DNA-50 for DNA.
 
-
#Wipe, apply sample to nib, measure
 
-
#Reblank about every 10 samples
 
-
#Blank with water at end, wipe and log off
 
-
 
-
 
-
 
# Log onto computer and select Nanodrop program from the desktop (ND 1000)
# Log onto computer and select Nanodrop program from the desktop (ND 1000)
-
# To clean Nanodrop wipe pedestal and top and add 3 µl of water to nib of pedestal on the spectrometer and press blank.
+
# To clean Nanodrop machine wipe pedestal and top and add 3 µl of water to nib of pedestal. Press blank.
# Wipe the water off, to initialise/equalizen the equipment add 3 μl of the elution buffer [EB] used in the sample and press blank. Set to DNA-50 for DNA.  
# Wipe the water off, to initialise/equalizen the equipment add 3 μl of the elution buffer [EB] used in the sample and press blank. Set to DNA-50 for DNA.  
# Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.  
# Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.  
# If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
# If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
-
# After measurement, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.  
+
# After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.
-
 
+
-
 
+
-
{|
+
-
|-
+
-
|[[Image:Newcastle_nanodrop_1.jpeg|thumb]]
+
-
|[[Image:Newcastle_nanodrop_2.jpeg|thumb]]
+
-
|[[Image:Newcastle_nanodrop_3.jpeg|thumb]]
+
-
|-
+
-
|}
+

Latest revision as of 09:20, 22 July 2010

Newcastle nanodrop 2.jpeg
Newcastle nanodrop 1.jpeg
Newcastle nanodrop 3.jpeg

Nanodrop can be used to measure the DNA, RNA and protein

Measure the concentration and purity of extracted DNA using absorbance (using the automated nanodrop machine!)

The ideal concentration of DNA is 150 ng/ml!

Method:

  1. Log onto computer and select Nanodrop program from the desktop (ND 1000)
  2. To clean Nanodrop machine wipe pedestal and top and add 3 µl of water to nib of pedestal. Press blank.
  3. Wipe the water off, to initialise/equalizen the equipment add 3 μl of the elution buffer [EB] used in the sample and press blank. Set to DNA-50 for DNA.
  4. Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
  5. If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
  6. After measurements, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.
Retrieved from "http://2010.igem.org/NanoDrop_Spectrophotometer"