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| - | <!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** --> | + | {{Template:HokkaidoU_Japan}} |
| | + | =Project Abstract= |
| | + | <p> |
| | + | Our project is on Type lll Secretion Apparatus which is one of the most amazing biological devices. It can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. This apparatus which looks like a syringe is an organelle of pathogenic gram-negative |
| | + | bacterium such as Salmonella and Yersinia. We are aiming at making this device available for E. coli. Because it will not involve usage of pathogenic strains, it will be safer to use. <!--To transfer T3SS functionally from Salmonella to E.coli it is essential to integrate at least 40kb of DNA fragment coding more than 20 proteins. So we will make suggestions about how to optimize E.coli transformation method for large size DNA fragments. -->Also we will show how to construct proteins for secretion and how to measure if it is really secreted using GFP. |
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| - | <div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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| - | <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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| - | This is a template page. READ THESE INSTRUCTIONS.
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| - | <div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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| - | <div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
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| - | </div>
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| - | <!-- *** End of the alert box *** -->
| + | ==The Experiments== |
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| - | |You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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| - | |[[Image:HokkaidoU_Japan_logo.png|200px|right|frame]]
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| - | ''Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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| - | |[[Image:HokkaidoU_Japan_team.png|right|frame|Your team picture]]
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| - | |align="center"|[[Team:HokkaidoU_Japan | Team Example]]
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| - | |}
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| - | <!--- The Mission, Experiments --->
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| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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| - | !align="center"|[[Team:HokkaidoU_Japan|Home]]
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| - | !align="center"|[[Team:HokkaidoU_Japan/Team|Team]]
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| - | !align="center"|[https://igem.org/Team.cgi?year=2010&team_name=HokkaidoU_Japan Official Team Profile]
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| - | !align="center"|[[Team:HokkaidoU_Japan/Project|Project]]
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| - | !align="center"|[[Team:HokkaidoU_Japan/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:HokkaidoU_Japan/Modeling|Modeling]]
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| - | !align="center"|[[Team:HokkaidoU_Japan/Notebook|Notebook]]
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| - | !align="center"|[[Team:HokkaidoU_Japan/Safety|Safety]]
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| - | |}
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| - | | + | ==Results== |
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| - | == '''Overall project''' ==
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| - | Your abstract
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| - | == Project Details==
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| - | === Part 2 ===
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| - | === The Experiments ===
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| - | === Part 3 ===
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| - | == Results == | + | |
Project Abstract
Our project is on Type lll Secretion Apparatus which is one of the most amazing biological devices. It can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. This apparatus which looks like a syringe is an organelle of pathogenic gram-negative
bacterium such as Salmonella and Yersinia. We are aiming at making this device available for E. coli. Because it will not involve usage of pathogenic strains, it will be safer to use. Also we will show how to construct proteins for secretion and how to measure if it is really secreted using GFP.
The Experiments
==Results==
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